延边黄牛黑皮质素受体-4基因多态性及其与肉质性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2017.05.022

Abstract

Abstract:

In order to explore the effects of the key gene of regulating cattle fat deposition on meat quality traits, screening candidate gene which affect beef fattening traits. This study employed 157 cattles (24 months old) with similar weight as research object, analyzed the correlation between SNPs of MC4R gene and meat quality traits in Yanbian Yellow cattle by PCR direct sequencing method. The results indicated that the mutation site of C→G had been found in MC4R gene 1 069 bp, which resulted in L (leucine) translated into V (valine). Protein secondary structure lost α-spiral and β-fold at the 166, 243 and 280 amino acid position, which resulted in the changes of spatial position of β-turns in tertiary structure. This site showed different degrees correlation with backfat thickness, lrightness, yellowness of Yanbian Yellow cattle. The backfat thickness and yellowness (3 d) of GG genotype were significantly higher than CC and CG genotypes (P <0.05). The lrightness of CC and CG genotypes were significantly higher than CC genotype (P <0.05). These results declared that the site had remarkably correlatived with Yanbian Yellow cattle relevant economic traits, and the mutation site could be used as a potential molecular marker.

Key words: Yanbian Yellow cattle; MC4R gene; SNPs; meat quality traits

CLC Number:

S813.3

FENG Jian, LI Ying-hua, YAN Chang-guo. The Correlation Analysis Between the SNPs of MC4R Gene and Meat Quality Traits in Yanbian Yellow Cattle[J]. , 2017, 44(5): 1406-1413.

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The Correlation Analysis Between the SNPs of MC4R Gene and Meat Quality Traits in Yanbian Yellow Cattle

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