伊犁马MyoG基因外显子1多态性与肉质性状及肌纤维性状关联分析

doi:10.16431/j.cnki.1671-7236.2021.07.026

Abstract

Abstract: In order to find molecular markers for meat quality of Yili horses,38 Yili horses were used to determine meat quality traits and muscle fiber traits (including water loss rate,cooked meat rate,shear force,muscle fiber cross-sectional area,muscle fiber diameter and muscle fiber density).The polymorphism of myogenin (MyoG) gene exon 1 in Yili horse population was detected by PCR direct sequencing,and the relationship between different genotypes of MyoG gene SNPs and meat quality,muscle fiber traits were analyzed.The results showed that five SNPs were detected in exon 1 of MyoG gene,including SNP1 (g.31187343 A>C),SNP2 (g.31187333 G>A),SNP3 (g.31187132 C>T),SNP4 (g.31187105 C>G) and SNP5 (g.31187099 C>T).The SNP1 was found to be a missense mutation,and the mutation of base A to C occurs,which maked the amino acid mutation from threonine to proline.The other sites were found to be nonsense mutations.SNP3 and SNP4 were found to be moderately polymorphic,while SNP1 and SNP2 were low polymorphic,the four SNPs were in Hardy-Weinberg equilibrium.Association analysis showed that:SNP1 and SNP4 genotypes in exon 1 of MyoG gene were significantly associated with water loss rate,cooked meat rate,muscle fiber cross-sectional area,muscle fiber diameter and muscle fiber density (P<0.05).SNP3 genotypes were significantly associated with cooked meat rate,muscle fiber cross-sectional area and muscle fiber density (P<0.05).There was no significant correlation between SNP2 genotypes and each index (P>0.05).In conclusion,five SNPs were detected in exon 1 of MyoG gene in Yili horse,among which SNP1 (g.31187343 A>C),SNP3 (g.31187132 C>T) and SNP4 (g.31187105 C>G) had significant effects on meat quality and muscle fiber traits.These SNPs might become molecular markers of meat quality in Yili horse.

Key words: Yili horse; MyoG gene; meat quality traits; muscle fiber traits; correlation

CLC Number:

S821.2

LI Yunxia, YAO Xinkui, MENG Jun, ZENG Yaqi, WANG Jianwen, ZHENG Guopeng, ZHANG Yue, YUAN Xinxin, WANG Tongliang, LI Yuntao. Correlation Analysis Between Polymorphism of MyoG Gene Exon 1 With Meat Quality Traits and Muscle Fiber Traits in Yili Horse[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2504-2511.

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Correlation Analysis Between Polymorphism of MyoG Gene Exon 1 With Meat Quality Traits and Muscle Fiber Traits in Yili Horse

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