湘村黑猪sirt2基因多态性及其与肉质性状的关联分析和组织表达研究

doi:10.16431/j.cnki.1671-7236.2019.04.015

Abstract

Abstract:

This study was aimed to investigate the expression of the silent information regulator family (SIRT1-7) sirt2geneof different tissues inXiangcunBlackpig,analyzetheassociation between sirt2 gene polymorphism and meat quality traits,and find the molecular markers associated with meat quality traits in Xiangcun Black pig.The PCR-RFLP method and gene sequencing technology were used to analyze the polymorphism of sirt2 gene in Xiangcun Black pig.Real-time PCR was used to analyze the relative expression of sirt2 gene in eight tissues including heart,liver,spleen,lung,kidney,pancreas,hind leg muscle and longissimus dorsi.At the same time,the different genotypes of sirt2 gene mutation site and muscle color value,pH_{45 min},pH_{24 h},drip loss,water loss rate,intramuscular fat,tenderness and loin eye muscle area were analyzed by SAS 9.4 software.The results showed that a mutation (C to T) was found at 240 bp in the amplified fragment of sirt2 gene exon 8 in Xiangcun Black pig,and the encoded amino acid changed from arginine (Arg) to cysteine (Cys).The mutation type was a missense mutation and formed three genotypes of CC,CT and TT,CC genotype was the dominant genotype,and C was the dominant allele.The χ² test indicated that the mutation site deviated from Hardy-Weinberg equilibrium.The homozygosity of this locus was higher,the number of effective alleles was 1.301,and the polymorphic information content was 0.205,which was low polymorphism (PIC<0.25).Correlation analysis between gene polymorphism and meat quality traits showed that the muscle L^* value and drip loss of TT genotype were significantly lower than that of CC and CT genotypes (P<0.05).The water loss rate of CC genotype was significantly higher than that of CT and TT genotypes (P<0.05).sirt2 gene was expressed in 8 tissues of Xiangcun Black pig,and the relative expression was the highest in longissimus dorsi,which was not significantly different with lung (P>0.05),but was significantly higher than heart,liver,spleen,kidney,pancreas and hind leg muscle (P<0.05),and the relative expressions in heart,liver,spleen,kidney and pancreas were not significant (P>0.05).The results showed that sirt2 gene had a certain effect on the development of meat quality traits in Xiangcun Black pig,which could be used as a candidate gene affecting the meat quality in Xiangcun Black pig.

Key words: Xiangcun Black pig; sirt2 gene; meat quality traits; tissue expression; association analysis

CLC Number:

S813.3

CUI Qingming, ZHANG Xing, PENG Yinglin, GAO Feng, CHEN Chen, DENG Yuan, REN Huibo, HU Xionggui, ZHU Ji, LUO Xuan, LIU Yingying, ZUO Jianbo. Study on Tissue Expression and Association Analysis Between sirt2 Gene Polymorphism and Meat Quality Traits in Xiangcun Black Pig[J]. , 2019, 46(4): 1086-1093.

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Study on Tissue Expression and Association Analysis Between sirt2 Gene Polymorphism and Meat Quality Traits in Xiangcun Black Pig

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