Abstract: This experiment was conducted to explore the association between perilipin (PLIN) gene and carcass and meat quality traits in Yanbian Yellow cattle.In this study,70 30-month-old healthy Yanbian Yellow cattle were used as experimental materials,the blood of jugular vein and its 12-13 intercostal longissimus muscle dorsi were collected to extract DNA samples of Yanbian Yellow cattle,and determine the carcass and meat quality characteristics.The SNP of PLIN gene was detected by direct sequencing,and the correlation analysis was conducted based on the carcass and meat quality data of Yanbian Yellow cattle and the genetic polymorphism of PLIN gene.The results showed that there were 2 SNPs of PLIN gene exon 3 in Yanbian Yellow cattle:g.103 C→T and g.156 T→C,both of them were missense mutations.g.103 C→T contained two genotypes:CC and CT,CC genotype was the dominant genotype,the genotype frequency was 0.81,C was the dominant allele,the gene frequency was 0.90.The intramuscular fat,pre slaughter weight,carcass weight and back fat thickness of CT genotype was significantly higher than those of CC genotype(P<0.05).g.156 T→C contained two genotypes:TT and TC,TT genotype was the dominant genotype,the genotype frequency was 0.83,T was the dominant allele,the gene frequency was 0.92.The pre slaughter weight,carcass weight and back fat thickness of TC genotype were significantly higher than those of TT genotype (P<0.05).Chi square test showed that the two SNPs of PLIN gene in Yanbian Yellow cattle reached Hardy-Weinberg equilibrium (P>0.05),and belonged to low polymorphism (PIC<0.25).In conclusion,g.103 C→T and g.156 T→C of PLIN gene had potential effect on carcass and meat quality traits in Yanbian Yellow cattle,and could be used as a candidate gene and potential molecular marker of Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; PLIN gene; polymorphism; carcass traits; meat quality traits