猪IGFBP2基因遗传多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2017.10.026

Abstract

Abstract:

This study was aimed to analyze the genetic variation of insulin-like growth factor binding protein 2 (IGFBP2) gene effect on meat quality traits in pigs. A pair of primer was designed,which based on DNA sequence of IGFBP2 gene (GenBank accession No.:BV727778). The genetic polymorphism of IGFBP2 gene was detected by PCR-RFLP in Sujiang pig, Jiangquhai pig and Landrace pig, and the association between IGFBP2 gene polymorphism and meat quality traits in three pig breeds was analyzed. The results showed that three genotypes (AA, AB and BB) were detected. The Chi-square test results showed that the genotype distribution of three pig breeds was in Hardy-Weinberg equilibrium (P > 0.05). The polymorphism information content (PIC) were moderately polymorphic in three pig breeds. According to the association analysis of different genotypes, significant difference was found in color value, intramuscular fat content and marbling, the color value (except for Jianquhai pig) and intramuscular fat content of AA genotype were significantly higher than AB and BB genotypes (P < 0.05), the marbling of AA and AB genotypes was significantly higher than BB genotype (P < 0.05). In conclusion,the polymorphism of IGFBP2 gene intron 3 had significant effects on meat quality traits, and was candidate gene for the potential impact on meat quality traits of pigs.

Key words: pigs; IGFBP2 gene; PCR-RFLP; meat quality traits

CLC Number:

S813.3

LIU Hai-xia, ZHU Ai-wen. Polymorphism of IGFBP2 Gene and Its Association with Meat Quality Traits in Pigs[J]. , 2017, 44(10): 3016-3022.

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Polymorphism of IGFBP2 Gene and Its Association with Meat Quality Traits in Pigs

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