小花棘豆中毒对和田羊母羊繁殖器官的影响

doi:10.16431/j.cnki.1671-7236.2017.10.025

Abstract

Abstract:

The study was aimed to research the effects of Oxytropis glabra DC (O.glabra DC) poisoning on reproductive organs coefficent, reproductive performance and related gene expression in Hetian sheep ewes. The sera, hypothalamus, pituitary and ovary of Hetian sheep ewes cases poisoning of O. glabra DC were collected, the organ exponent and contents of GnRH, FSH, LH, E₂ and P₄ in serum were measured, then the mRNA expression of reproductive genes were measured. The result demonstrated that the index of hypothalamus, pituitary and ovary were extremely significantly higher than normal sheep (P < 0.01),the follicle number on the surface of the ovaries in poisoning groups were significantly lower than normal sheep (P < 0.05). The result of hematoxylin-eosin staining showed that the nuclear of neuronal cells in hypothalamus were pyknosis and hyperchromatic; The nuclear of cells in pituitary deformation and hyperchromatic, cytoplasm decreased; The primary oocyte in ovary dissolved, disappeared, interstitial blood vessel expanded. The content of GnRH, FSH, LH, E₂ and P₄ in serum in poisoned Hetian sheep ewes were extremely significantly lower than normal sheep (P < 0.01). The mRNA expression levels of Kiss-1, GPR54, ERα in hypothalamus, GnRHR in pituitary and FSHR and LHR in ovary were extremely significantly lower than normal sheep (P < 0.01). The result showed that O. glabra DC poisoning could affect Hetian sheep ewes reproductive system by affecting microstructure and ultrastructure of hypothalamus-pituitary-ovarian axis.

Key words: Oxytropis glabra DC; poisoning; Hetian sheep; reproductive organs

CLC Number:

S858.26

YAO Na, JIA Qi-zhen, CHEN Gen-yuan, MAIMAITI·Yasen, WANG Shuai. Effects of Oxytropis glabra DC Poisoning on the Reproductive Organs in Hetian Sheep Ewes[J]. , 2017, 44(10): 3009-3015.

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Effects of Oxytropis glabra DC Poisoning on the Reproductive Organs in Hetian Sheep Ewes

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