蜡样芽孢杆菌检测方法研究进展

doi:10.16431/j.cnki.1671-7236.2018.03.010

Abstract

Abstract:

Bacillus cereus is a common bacterium causing food poisoning,gram-positive opportunistic pathogen,and widely distributing in soil,water and all kinds of food.In recent years,Bacillus cereus also has been found in a variety of animals including people as a zoonotic bacterium.It gives rise to gastrointestinal disease,which leading to diarrhoeal or emetic type of food poisoning.Thus,a rapid and accurate detection is critical for controlling the pollution or the treatment.This study conducted a comprehensive and detailed summary for the detection methods of Bacillus cereus, including conventional detection,conventional PCR,multiplex PCR,Real-time PCR,propidium monoazide-quantitative PCR,droplet digital PCR,ring-mediated isothermal amplification technology and enzyme-linked immunosorbent assay (ELISA).From the traditional method to the emerging technology,involving the traditional detection technology,molecular biology detection and immunological detection technology,the authors described the principle,range and the advantages and disadvantages of each method.Because of the difference in sensitivity,accuracy,sample requirements and so on,the detection methods could be selected according to the requirement and conditions.In the combination of molecular biology methods and immunological methods,the samples are detected from multiple angles and multilevels,and the results can be presented in a comprehensive and convincing manner.Moreover, Bacillus cereus can produce a variety of toxins which determine its pathogenicity.Thus,the detection of toxin contributes to determining the pathogen and its pathogenicity.Overall,as a threat to the health of mammals and humans,rapid and accurate detection of Bacillus cereus can effectively assist treatment and advance prevention.The detection methods were summarized here to contribute a comprehensive and accurate assessment of the risk of Bacillus cereus, and it was respected to provide a scientific evidence to active monitoring and early warning.

Key words: Bacillus cereus; detection; food poisoning

CLC Number:

S852.61⁺6

HUANG Jingjing, LUO Jing, HE Hongxuan. Research Progress on Detection Methods for Bacillus cereus[J]. , 2018, 45(3): 635-642.

References

[1] MILLER R A,BENO S M,KENT D J,et al.Bacillus wiedmannii sp.nov.,a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments[J].International Journal of Systematic and Evolutionary Microbiology,2016,66(11):4744-4753.
[2] DIDIER A,JESSBERGER N,KREY V,et al.The mutation Glu151Asp in the B-component of the Bacillus cereus non-hemolytic enterotoxin (Nhe) leads to a diverging reactivity in antibody-based detection systems[J].Toxins, 2015,7(11):4655-4667.
[3] CIESLIK P,KNAP J,KOLODZIEJ M,et al.Real-time PCR identification of unique Bacillus anthracis sequences[J].Folia Biologica (Praha),2015,61(5):178-183.
[4] LIU Y,LAI Q,GOKER M,et al.Genomic insights into the taxonomic status of the Bacillus cereus group[J].Scientific Reports,2015,5:14082.
[5] CEUPPENS S,BOON N,Uyttendaele M.Diversity of Bacillus cereus group strains is reflected in their broad range of pathogenicity and diverse ecological lifestyles[J].FEMS Microbiology Ecology,2013,84(3):433-450.
[6] 李浇,郑之琬,何娟,等.重庆荣昌某奶牛场子宫内膜炎蜡样芽孢杆菌的分离鉴定及药敏试验[J].中国兽医杂志,2012,48(6):52-54. LI J,ZHENG Z W,LI J,et al.Isolation,identification and drug sensitivity test of Bacillus cereus from endometritis in a dairy farm in Rongchang[J].Chinese Journal of Veterinary Medicine,2012,48(6):52-54.(in Chinese)
[7] 赵振宇,戴荣四,刘东友,等.一株猪源致病性蜡样芽孢杆菌的分离与鉴定[J].湖南农业大学学报(自然科学版),2014,3:311-315. ZHAO Z Y,DAI R S,LIU D Y,et al.Isolation and characterization of a pathogenic Bacillus cereus from a piglet with diarrhea[J].Journal of Hunan Agricultural University (Natural Sciences),2014,3:311-315.(in Chinese)
[8] 骆艺文,郝志凯,王印庚,等.一株引起刺参"腐皮综合征"的蜡样芽孢杆菌[J].水产科技情报,2009,2:60-63. LUO Y W,HAO Z K,WANG Y G,et al.A strain of Bacillus cereus that causes apostichopus japonicus "rot skin syndrome"[J].Fisheries Science & Technology Information,2009,2:60-63.(in Chinese)
[9] HONG M,WANG Q,TANG Z,et al.Association of genotyping of Bacillus cereus with clinical features of post-traumatic endophthalmitis[J]. PLoS One,2016,11(2):e147878.
[10] ABFALTER C M,SCHONAUER E,PONNURAJ K,et al.Cloning,purification and characterization of the collagenase ColA expressed by Bacillus cereus ATCC 14579[J].PLoS One,2016,11(9):e162433.
[11] YEO I C,LEE N K,CHA C J,et al.Interspecies interaction of signal peptide PapR secreted by Bacillus cereus and its effect on production of antimicrobial peptide[J].Applied Biochemistry and Biotechnology,2012,166(3):700-710.
[12] 刘水,杜志刚.VITEK-2全自动微生物鉴定系统在蜡样芽孢杆菌检测中应用[J].中国误诊学杂志,2010,24:5854-5855. LIU S,DU Z G.Application of VITEK-2 automatic microbiological identification system in detection of Bacillus cereus[J].Chinese Journal of Misdiagnostics,2010,24:5854-5855.(in Chinese)
[13] LI G N,XIA X J,ZHAO H H,et al.Identification and characterization of Bacillus cereus SW7-1 in Bombyx mori (Lepidoptera:Bombycidae)[J].Journal of Insect Science,2015,15(1):136.
[14] CAAMANO-ANTELO S,FERNANDEZ-NO I C,BOHME K,et al.Genetic discrimination of foodborne pathogenic and spoilage Bacillus spp.based on three housekeeping genes[J].Food Microbiology,2015,46:288-298.
[15] CARROLL L M,KOVAC J,MILLER R A,et al.Rapid,high-throughput identification of anthrax-causing and emetic Bacillus cereus group genome assemblies using BTyper,a computational tool for virulence-based classification of Bacillus cereus group isolates using nucleotide sequencing data[J].Applied and Environmental Microbiology,2017,83(9):e01096-17.
[16] TANG V H,CHANG B J,SRINIVASAN A,et al.Skin-associated Bacillus,staphylococcal and micrococcal species from the house dust mite,dermatophagoides pteronyssinus and bacteriolytic enzymes[J].Experimental and Applied Acarology,2013,61(4):431-447.
[17] WALKER-YORK-MOORE L,MOORE S C,FOX E M.Characterization of enterotoxigenic Bacillus cereus sensu lato and Staphylococcus aureus isolates and associated enterotoxin production dynamics in milk or meat-based broth[J].Toxins,2017,9(7):225.
[18] KIM M J,HAN J K,PARK J S,et al.Various enterotoxin and other virulence factor genes widespread among Bacillus cereus and Bacillus thuringiensis strains[J].Journal of Microbiology and Biotechnology,2015,25(6):872-879.
[19] REIS A L,MONTANHINI M T,BITTENCOURT J V,et al.Gene detection and toxin production evaluation of hemolysin BL of Bacillus cereus isolated from milk and dairy products marketed in Brazil[J].Brazilian Journal of Microbiology,2013,44(4):1195-1198.
[20] HUILLET E,TEMPELAARS M H,ANDRE-LEROUX G,et al.PlcRa,a new quorum-sensing regulator from Bacillus cereus,plays a role in oxidative stress responses and cysteine metabolism in stationary phase[J].PLoS One,2012,7(12):e51047.
[21] OLTUSZAK-WALCZAK E,WALCZAK P.PCR detection of cytK gene in Bacillus cereus group strains isolated from food samples[J].Journal of Microbiological Methods,2013,95(2):295-301.
[22] KAMINSKA P S,YERNAZAROVA A,Murawska E,et al.Comparative analysis of quantitative reverse transcription Real-time PCR and commercial enzyme imunoassays for detection of enterotoxigenic Bacillus thuringiensis isolates[J].FEMS Microbiology Letters,2014,357(1):34-39.
[23] NASRABADI Z,RANJBAR R,POORALI F,et al.Detection of eight foodborne bacterial pathogens by oligonucleotide array hybridization[J].Electronic Physician,2017,9(5):4405-4411.
[24] LEE N,KWON K Y,OH S K,et al.A multiplex PCR assay for simultaneous detection of Escherichia coli O157:H7,Bacillus cereus,Vibrio parahaemolyticus,Salmonella spp.,Listeria monocytogenes,and Staphylococcus aureus in Korean ready-to-eat food[J].Foodborne Pathogens and Disease,2014,11(7):574-580.
[25] ARSLAN S,EYI A,KUCUKSARI R.Toxigenic genes,spoilage potential,and antimicrobial resistance of Bacillus cereus group strains from ice cream[J].Anaerobe,2014,25:42-46.
[26] KUMAR T D K,MURALI H S,BATRA H V.Multiplex PCR assay for the detection of enterotoxic Bacillus cereus group strains and its application in food matrices[J].Indian Journal of Microbiology,2010,50(2):165-171.
[27] KIM J H,RHIM S R,KIM K T,et al.Simultaneous detection of listeria monocytogenes,Escherichia coli O157:H7,Bacillus cereus,Salmonella spp.,and Staphylococcus aureus in low-fatted milk by multiplex PCR[J].Korean Journal for Food Science of Animal Resources,2014,34(5):717-723.
[28] CREMONESI P,PISANI L F,LECCHI C,et al.Development of 23 individual TaqMan^® Real-time PCR assays for identifying common foodborne pathogens using a single set of amplification conditions[J]. Food Microbiology,2014,43:35-40.
[29] FORGHANI F,WEI S,OH D H.A rapid multiplex Real-time PCR high-resolution melt curve assay for the simultaneous detection of Bacillus cereus,Listeria monocytogenes,and Staphylococcus aureus in food[J]. Journal of Food Protection,2016,79(5):810-815.
[30] CATTANI F,BARTH V J,NASARIO J S,et al.Detection and quantification of viable Bacillus cereus group species in milk by propidium monoazide quantitative Real-time PCR[J].Journal of Dairy Science,2016,99(4):2617-2624.
[31] PORCELLATO D,NARVHUS J,SKEIE S B.Detection and quantification of Bacillus cereus group in milk by droplet digital PCR[J].Journal of Microbiological Methods,2016,127:1-6.
[32] LI F,YAN W,LONG L,et al.Development and application of loop-mediated isothermal amplification assays for rapid visual detection of cry2Ab and cry3A genes in genetically-modified crops[J].International Journal of Molecular Sciences,2014,15(9):15109-15121.
[33] 周巍,张薇,刘亮,等.环介导等温扩增技术检测酸乳中蜡样芽孢杆菌[J].乳业科学与技术,2013,5:29-31. ZHOU W,ZHANG W,LIU L,et al.Detection of Bacillus cereusin yogurt by loop-mediated isothermal amplification assay[J].Journal of Dairy Science & Technology, 2013,5:29-31.(in Chinese)
[34] ZHU L,HE J,CAO X,et al.Development of a double-antibody sandwich ELISA for rapid detection of Bacillus cereus in food[J].Scientific Reports,2016,6:16092.
[35] CEUPPENS S,RAJKOVIC A,HAMELINK S,et al.Enterotoxin production by Bacillus cereus under gastrointestinal conditions and their immunological detection by commercially available kits[J].Foodborne Pathogens and Disease,2012,9(12):1130-1136.
[36] TALLENT S M,HAIT J M,BENNETT R W.Analysis of Bacillus cereus toxicity using PCR,ELISA and a lateral flow device[J].Journal of Applied Microbiology,2015,118(4):1068-1075.
[37] KRAUSE N,MORAVEK M,DIETRICH R,et al.Performance characteristics of the Duopath^® cereus enterotoxins assay for rapid detection of enterotoxinogenic Bacillus cereus strains[J].International Journal of Food Microbiology,2010,144(2):322-326.
[38] STENFORS ARNESEN L P,FAGERLUND A,GRANUM P E.From soil to gut:Bacillus cereus and its food poisoning toxins[J].FEMS Microbiol Review,2008,32(4):579-606.
[39] SALEH M,AL N M,DOLOY A,et al.Bacillus cereus,an unusual cause of fulminant liver failure:Diagnosis may prevent liver transplantation[J].Journal of Medical Microbiology,2012,61(Pt 5):743-745.
[40] ZHU K,HOLZEL C S,CUI Y,et al.Probiotic Bacillus cereus strains,a potential risk for public health in China[J].Frontiers in Microbiology,2016,7:718.

Research Progress on Detection Methods for Bacillus cereus

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