小分子化合物单克隆抗体的制备及其在动物医学领域的应用

doi:10.16431/j.cnki.1671-7236.2021.03.037

Abstract

Abstract: The trace detection of pesticides,veterinary drugs,heavy metals,biotoxins and other residual substances is of great significance in the fields of medical diagnosis,food safety and environmental monitoring.The immunoassays based on antigen-antibody specific recognition have advantages of strong specificity,high sensitivity and good stability,simple and quick operation,etc.,but how to obtain specific antibodies against small molecular compounds is the key to develop immunoassays.In this paper,the hybridoma technology for the preparation of monoclonal antibody of small molecular compounds was firstly introduced.Important factors affecting the production of monoclonal antibody of small molecular compounds in hybridoma technology was then briefly analyzed.The applications of monoclonal antibody in veterinary medicine,such as veterinary drug residues detection,drug development and environmental protection,were reviewed.Finally,the development trend of the monoclonal antibody production and application was prospected.This review provided guidance for the preparation and application of the monoclonal antibodies of small molecular compound.

Key words: small molecular compounds; trace detection; monoclonal antibody; influence factor; veterinary clinic

CLC Number:

YANG Songxin, FENG Jianyuan, GUO Xuan, WEI Liuyuan, LIANG Yiying, YAN Hao, ZHANG Ziyi, CHEN Hailan. Preparation of Monoclonal Antibodies for Small Molecular Compounds and Their Applications in the Field of Veterinary Medicine[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1121-1131.

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Preparation of Monoclonal Antibodies for Small Molecular Compounds and Their Applications in the Field of Veterinary Medicine

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