高坡猪肌肉生长抑制素基因多态性及其与肉质性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2017.04.023

Abstract

Abstract:

To explore the influence of Gaopo pig myostatin (MSTN) gene polymorphism on meat quanlity traits (moisture,crude fat,crude protein,crude ash, loin muscle area, marbling score, pH value, color of meat,tenderness,drip loss, water loss rate), MSTN gene was chosen as a candidate gene for research meat quality traits in this study. 50 Gaopo pigs at 10 months old were selected,the single nucleotide polymorphism (SNP) of three exons of MSTN gene in Gaopo pig were detected by direct sequencing of PCR amplification products, and the correlation between MSTN gene SNP and meat quality traits was analyzed using SPSS 20.0 software. The results showed that a C/T mutation had been found at 63 bp of MSTN gene exon 3,the site was silent mutation, and did not caused changes of encoding amino acid. Genotype analysis showed only three samples had mutations in the site, CC genotype was the dominant type and allele C was the dominant gene, but there was no TT homozygous genotype. The test of Hardy-Weinberg equilibrium showed the research group obeyed the genetic equilibrium (P>0.05). Population genetic parameter analysis showed the heterozygosity (He) of C63T was lower, and had a low variation in Gaopo pig population. Polymorphism information content (PIC) analysis showed that it was a low polymorphic loci (PIC<0.25), and the site could provide a little genetic imformation. There was no significant difference between the different genotypes and meat quality traits (P>0.05).Given the above, the polymorphism of MSTNgene exon was found in Gaopo pig, with less variation and relatively conservative.It's needed to expand the number of sample for further explore whether the site could be considered as genetic markers for meat quality traits of Gaopo pig or not.

Key words: Gaopo pig; MSTN gene; meat quality traits

CLC Number:

S813.3

YI Heng-jie, LI Hui, ZHAO Zhong-hai, YANG Hua-ting, PENG Bang-xing, WANG Yu. The Polymorphisms of MSTN Gene and Its Relationship with Meat Quanlity Traits in Gaopo Pig[J]. , 2017, 44(4): 1102-1107.

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The Polymorphisms of MSTN Gene and Its Relationship with Meat Quanlity Traits in Gaopo Pig

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