牛源多杀性巴氏杆菌Oma87基因的分子特征、抗原性及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2020.07.025

Abstract

Abstract: In order to reveal the molecular characteristics and phylogenetic analysis of Oma87 gene of Pasteurella multocida (Pm),the Oma87 gene in Pm8 (capsular serotype A) was amplified and cloned,the sequence and antigenicity was analyzed by bioinformatics software and conducted to construct phylogenetic tree in this study.The results showed that the length of the Oma87 gene was 2 376 bp,which encoded 791 amino acids.Amino acid sequence analysis found that the Oma87 protein was an acidic protein with hydrophilicity,which had high stability.It had a signal peptide but without transmembrane domain.The secondary and tertiary structure analysis found that the Oma87 protein was "N" type three-dimensional structure formed by random coils connected multiple extended strands.The nucleotide homology analysis result showed that the Oma87 gene of Xinjiang isolated strain Pm8 was highly homologous with bovine Pm (capsular serogroup A) isolated and part of swine Pm (capsular serogroup A) from other different geographic regions,but not highly homologous to other capsular serotype group.VaxiJen classified Oma87 protein as probable antigen because it had a value of 0.5478 which was higher than the normal threshold value of 0.4,proved that Oma87 protein could act as the immunogenic protein,which laid a foundation for further study on Oma87 protein as Pm vaccine candidate antigen.

Key words: Pasteurella multocida; Oma87 gene; molecular characteristics; antigenicity; phylogenetic

CLC Number:

Q786

ZHANG Xingxing, HUANG Xin, HE Yanhua, HE Lixiong, HAN Mengli, ZHANG Qian, WU Tongzhong, ZHONG Fagang. Molecular Characteristics,Antigenicity and Phylogenetic Analysis of Oma87 Gene of Pasteurella multocida in Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2200-2206.

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Molecular Characteristics,Antigenicity and Phylogenetic Analysis of Oma87 Gene of Pasteurella multocida in Cattle

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