中国地方绵羊群体TYRP1基因遗传变异研究

doi:10.16431/j.cnki.1671-7236.2018.10.014

Abstract

Abstract:

This study was aimed to understand the population genetic variation and association relationship between the mutation sites of tyrosinase related protein 1(TYRP1) gene and different coat color phenotype in Chinese indigenous sheep populations.The directly sequencing and PCR-RFLP method were performed to identify single nucleotide polymorphisms (SNP) and genotype of TYRP1 gene,and then constructed haplotype,linkage disequilibrium analysis and genetic variation analysis were performed out by Beagle,PLINK and POPGENE softwares.The results showed that a total of 13 SNPs were identified in TYRP1 gene.10 SNPs of TYRP1 gene exons were chosen and used for genotyping in 10 sheep breeds.The results demonstrated that 10 sheep breeds presented variation at most mutation sites,except for some mutation sites had not polymorphisms in Large-tail Han sheep,China Merino sheep and Minxian Black-fur sheep.These results indicated that Chinese indigenous sheep populations had higher genetic diversity.The haplotypes analysis results indicated that a total of 42 haplotypes were detected in all sheep individuals,0000000000 (245/918) and 0100000001 (91/918) dominant haplotypes were detected in all sheep populations,except 0101100000 (93/918) was not detected in China Merino sheep,0001000001 (69/918) was not detected in China Merino sheep and Minxian Black-fur sheep,which were detected in other sheep populations.Linkage analysis results demonstrated that two block were detected in 10 SNPs of TYRP1 gene from all the sheep individuals.The genetic variation investigation indicated that Chinese sheep breeds had maintained a high level of within-population genetic differentiation,and the distinct genetic differentiation patterns and genetic relationships among the Chinese sheep breeds displayed a high consistency with the traditional classification.This study provided the theoretical basis for the further application of TYRP1 gene in coat color genetic traits of sheep.

Key words: sheep; TYRP1 gene; single nucleotide polymorphisms (SNP); genetic variation; coat color

CLC Number:

S813.3

YANG Guangli, FU Dongli, LANG Xia, WANG Yutao, WANG Qiankun, LUO Yuzhu. Study on Genetic Variation of TYRP1 Gene in Chinese Indigenous Sheep Populations[J]. , 2018, 45(10): 2772-2786.

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Study on Genetic Variation of TYRP1 Gene in Chinese Indigenous Sheep Populations

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