摩拉水牛二酰甘油酰基转移酶2基因的多态性检测

doi:10.16431/j.cnki.1671-7236.2018.10.015

Abstract

Abstract:

This study was aimed to investigate the single nucleotide polymorphisms(SNP) of DGAT2 gene in buffalo,and explore the population genetic characteristics of the polymorphism in Murrah buffalo.A total of 57 Murrah buffaloes from Guangxi buffalo research institute were selected as materials,the exon 2,introns 2 and 3 sequences of DGAT2 gene were amplified by PCR,SNP was detected using conventional sequencing,gene frequency and genotype frequency,polymorphism information content (PIC),effective number of alleles (Ne) and detection of genetic heterozygosity (He) were analyzed by genetic diversity analysis software (POPGENE) and SPSS softwares.The results showed that 9 SNPs loci were found of DGAT2 gene exon 2,introns 2 and 3 in Murrah buffalo (IVS2.54 G > A,IVS2.158 A > G,EVS2.191 A > G,EVS2.228 A > G,IVS3.311 C > T,IVS3.444 A > G,IVS3.451 A > C,IVS3.466 C > T and IVS3.521 C > T),EVS2.191 A > G caused the amino acid to be changed from isoleucine to valine,and there was a certain degree of linkage inheritance between the mutation sites but close to the chain equilibrium state.There was more difference between two allele frequency of 6 SNPs loci (IVS2.158 A > G,EVS2.191 A > G,IVS3.311 C > T,IVS3.451 A > C,IVS3.466 C > T and IVS3.521 C > T).It was suggested that the higher allele frequency individuals might be more suitable for survival.9 SNPs loci in DGAT2 gene in the buffalo species were high polymorphism,the heterozygosity were 0.1744 to 0.4975,which illustrated the buffalo populations DGAT2 gene genetic polymorphism was rich,and had a great breeding value and strain improvement potential.

Key words: Murrah buffalo; DGAT2 gene; polymorphism

CLC Number:

S813.3

YAN Shengfei, SHANG Jianghua, HUANG Lihua, YANG Chunyan, ZHENG Haiying, LI Mengqi, YU Nongqi, Mahmoud Moussa, QIN Guangsheng, HUANG Jiaxiang, ZHANG Xiufang. SNP Detection of DGAT2 Gene in Murrah Buffalo[J]. , 2018, 45(10): 2787-2796.

References

[1] BUHMAN K K,SMITH S J,STONE S J,et al.DGAT1 is not essential for intestinal triacylglycerol absorption or chylomicron synthesis[J].Journal of Biological Chemistry,2002,277(28):25474-25479.
[2] YU Y H,GINSBERG H N.The role of acyl-CoA:Diacylglycerol acyltransferase (DGAT) in energy metabolism[J].Annals of Biomedical Engineering,2004,36(4):252-261.
[3] HALL A M,KOU K,CHEN Z,et al.Evidence for regulated monoacylglycerol acyltransferase expression and activity in human liver[J].Journal of Lipid Research,2012,53(5):990-999.
[4] YU X X,MURRAY S F,PANDEY S K,et al.Antisense oligonucleotide reduction of DGAT2 expression improves hepatic steatosis and hyperlipidemia in obesemice[J].Hepatology,2005,42(2):362-371.
[5] LIU Y,MILLAR J S,CROMLEY D A,et al.Knockdown of acyl-CoA:Diacylglycerol acyltransferase 2 with antisense oligonucleotide reduces VLDL TG and ApoB secretion in mice[J].Biochimica et Biophysica Acta-biomembranes,2008,1781(3):97-104.
[6] 臧丽.猪DGAT2基因3'-UTR区的多态性鉴定及功能初步分析[D].泰安:山东农业大学,2011.ZANG L.Identification and characterization of polymorphisms in the 3'-UTR of porcine DGAT2 gene[D].Tai'an:Shandong Agricultural University,2011.(in Chinese)
[7] 毛海霞,陈宏,陈付英,等.郏县红牛DGAT2基因多态性与生长性状的相关性分析[J].遗传,2008,30(3):329-332.MAO H X,CHEN H,CHEN F Y,et al.Association of polymorphisms of DGAT2 gene with growth traits in Jiaxian Red cattle[J].Hereditas,2008,30(3):329-332.(in Chinese)
[8] 付金秀,郭红威,李超,等.鸡DGAT2基因第7外显子多态性与生产性能的关联[J].农业生物技术学报,2016,24(5):689-696.FU J X,GUO H W,LI C,et al.Polymorphism of exon 7 of DGAT2 gene and its association with growth traits in chicken[J].Journal of Agricultural Biotechnology,2016,24(5):689-696.(in Chinese)
[9] 张争锋,陈宏,李秋玲,等.南阳牛DGAT2基因PCR-RFLP多态性及其与生长性状相关性研究[J].遗传,2007,29(8):945-950.ZHANG Z F,CHEN H,LI Q L,et al.Polymorphisms of DGAT2 gene and its associations with several growth traits in Nanyang cattle[J].Hereditas,2007,29(8):945-950.(in Chinese)
[10] 鄢胜飞,张秀芳,黄玥萌,等.水牛Smad4基因的克隆及其真核表达载体的构建[J].中国畜牧兽医,2017,44(8):2369-2377.YAN S F,ZHANG X F,HUANG Y M,et al.Cloning and construction of eukaryotic expression vector of buffalo Smad4 gene[J].China Animal Husbandry & Veterinary Medicine,2017,44(8):2369-2377.(in Chinese)
[11] 郭亚芬,范晶,杨炳壮,等.摩拉水牛基因组DNA的RAPD分析[J].黑龙江畜牧兽医,2010,1:31-32.GUO Y F,FANG J,YANG B Z,et al.RAPD analysis of Murrah buffalo genome DNA[J].Heilongjiang Animal Science and Veterinary Medicine,2010,1:31-32.(in Chinese)
[12] STONE S J,MYERS H M,WATKINS S M,et al.Lipopenia and skin barrier abnormalities in DGAT2-deficient mice[J].Journal of Biological Chemistry,2004,279(12):11767-11776.
[13] WAKIMOTO K,CHIBA H,MICHIBATA H,et al.A novel diacylglycerol acyltransferase (DGAT2) is decreased in human psoriatic skin and increased in diabetic mice[J].Biochemical and Biophysical Research Communications,2003,310(2):296-302.
[14] 袁峥嵘,柳小春,马海明,等.二脂酰甘油酰基转移酶2(DGAT2)基因研究进展[J].遗传,2008,30(3):289-294.YUAN Z R,LIU X C,MA H M,et al.Research progress ondiacylgycerol acyltransferase 2(DGAT2) gene[J].Hereditas,2008,30(3):289-294.(in Chinese)
[15] WINTER A,ECKEVELD M,BININDA-EMONDS O R,et al.Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals[J].Cytogenetic and Genome Research,2003,102(1-4):42-47.
[16] JEONG J,KWON E G,IM S K,et al.Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers[J].Journal of Animal Science,2012,90(6):2044-2053.
[17] CONTE G,MELE M,CHESSA S,et al.Diacylglycerol acyltransferase 1,stearoyl-CoA desaturase 1,and sterol regulatory element binding protein 1 gene polymorphisms and milk fatty acid composition in Italian Brown cattle[J].Journal of Dairy Science,2010,93(2):753-763.
[18] YIN Q,YANG H,HAN X,et al.Isolation,mapping,SNP detection and association with backfat traits of the porcine CTNNBL1 and DGAT2 genes[J].Molecular Biology Reports,2012,39(4):4485-4490.
[19] 房兴堂,徐海霞,陈宏,等.徐淮白山羊DGAT2基因内含子3多态性与生长性状关联分析[J].生物技术通讯,2009,20(6):814-817.FANG X T,XU H X,CHEN H,et al.Association analysis on polymorphisms of DGAT2 gene intron 3 with growth traits in Xuhuai White goat[J].Letters in Biotechnology,2009,20(6):814-817.(in Chinese)
[20] 刘新武.中国荷斯坦奶牛DGAT2、Leptin基因多态性与产奶性状关系研究[D].杨凌:西北农林科技大学,2008.LIU X W.Study on relationship between polymorphism of DGAT2,Leptin genes and milk production in Chinese Holstein[D].Yangling:Northwest A & F University,2008.(in Chinese)
[21] 谢建鹏,石斌刚,胡江,等.牦牛DGAT2基因intron 5和intron 6多态性及其对乳品质性状的影响[J].农业生物技术学报,2017,25(5):758-769.XIE J P,SHI B G,HU J,et al.Polymorphism in the intron 5 and intron 6 of DGAT2 gene and its association with milk quality traits in yak (Bos grunniens)[J].Journal of Agricultural Biotechnology,2017,25(5):758-769.(in Chinese)

SNP Detection of DGAT2 Gene in Murrah Buffalo

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[2]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[3]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[4]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[5]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[6]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[7]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.
[8]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[9]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[10]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[11]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[12]	ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.
[13]	LIU Xinyong, TAN Zhengzhun, LI Hui, HUANG Jian, LUO Hua, ZENG Linghu, CHENG Juanru, ZHONG Huapei, YANG Chunyan, WANG Xiaoli, QIN Guangsheng. Effects of Heat Stress on Blood Biochemical Indexes of Murrah and Nili-Ravi Buffaloes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 521-528.
[14]	LI Mingming, WU Qingwei, DENG Yuting, JIN Shengwei, HE Na, ZHANG Junxia. Correlation Analysis of BMPRⅡ Gene Polymorphism and Its Haplotype with Lambing Traits in Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 587-597.
[15]	WANG Yingtong, YANG Suan, TAN Yuancheng, WANG Chunyuan, ZHANG Yiyu. Polymorphism of SLC8A1 Gene and Its Effect on Eggshell Quality in Changshun Blue-eggshell Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4688-4696.