基于LC-MS代谢组学的植物乳杆菌发酵黄芪的代谢产物分析

doi:10.16431/j.cnki.1671-7236.2021.09.019

Abstract

Abstract: This study was aimed to analyze the metabolites of fermented Astragalus by Lactobacillus plantarum using liquid chromatograph-mass spectrometer (LC-MS) metabonomics technology, and explore the mechanism of its interaction fermentation. The fermented (FT group) and unfermented (CT group) Astragalus solid powders were collected, respectively, and find out different metabolites and metabolic pathways through the sample pretreatment, LC-MS analysis and bioinformatics analysis. The result showed that the peak map of the total ion current map had good reproducibility. The main metabolites of fermented Astragalus had identified 183 metabolic components. The PCA map of the positive ion and negative ion mode samples could be distinguished well. The volcanic map analysis showed the metabolite changes were different between FT and CT groups, there were 1 416 metabolites up-regulated in positive ion model were enriched to 83 metabolic pathways, 935 metabolites down-regulated were enriched to 83 metabolic pathways;And 1 040 metabolites up-regulated in negative ion model were enriched to 52 metabolic pathways, 809 metabolites down-regulated were enriched to 45 metabolic pathways. The differential metabolites of fermented Astragalus acid, lipids, ketones and other amino acids were significantly increased, and olefins and other amino acids were significantly down-regulated, among them, 15 were up-regulated metabolites, and 2 were down-regulated metabolites. The key metabolites were mainly α-diethyl sulfate, 2-methyl citric acid, 3-isopropenyl-6-oxoheptanoic acid, etc., involving pyruvate metabolism, propionic acid metabolism, galactose metabolism and other pathways. The results provided a theoretical basis for the metabolites, fermentation interaction mechanism and clinical application of fermented Astragalus.

Key words: Lactobacillus plantarum; Astragalus; fermentation; LC-MS; metabolomics

CLC Number:

S816.6

QIAO Hongxing, ZHANG Liheng, ZHANG Xiaojing, SONG Yuzhen, BIAN Chuanzhou. Metabonomics Analysis of Fermented Astragalus by Lactobacillus plantarum Based on LC-MS Metabonomics[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3283-3292.

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Metabonomics Analysis of Fermented Astragalus by Lactobacillus plantarum Based on LC-MS Metabonomics

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