肉鸡弯曲杆菌噬菌体的筛选及应用

doi:10.16431/j.cnki.1671-7236.2021.09.018

Abstract

Abstract: The aim of the experiment was to screen out the lytic bacteriophages of Campylobacter and to explore their rational application in broiler production. Campylobacter bacteriophage screening samples were collected from broiler slaughterhouse, and specific Campylobacter bacteriophages were obtained by co-incubating, proliferating and purifying with local Campylobacter epidemic strains. The biological characteristics (host range, lethal curve, morphological characteristics, etc.) of Campylobacter bacteriophage were identified. As a feed additive, it was added to the diets of 38-day-old male Ross 308 broilers with 5×10⁷, 1×10⁸, 5×10⁸, 1×10⁹ and 5×10⁹ PFU/d 5 different amounts to observe the scavenging effect of Campylobacter in cecal contents of broilers. Finally, by comparing the effects of different phage ratio on the clearance rate of Campylobacter and the growth performance of broilers, the application scheme was established. The results showed that a total of 12 strains of Campylobacter jejuni bacteriophages were isolated from local broiler slaughterhouses using Campylobacter jejuni L26 as host bacteria. The bacteriophages BP11 and BP12 which had wide lytic spectrum and large plaque were chose by co-incubating, purifying and proliferating with 7 strains of Campylobacter jejuni from broiler farms. By morphological identification, the two bacteriophages were in accordance with the characteristics of Myoviridae and had a wide lytic spectrum, but their lytic ability of the same host bacteria was different. The bactericidal effect was significant when the BP11 and BP12 addition amount were 1×10⁹ and 5×10⁸ PFU/d, respectively. Bacteriopages BP11 and BP12 mixed addition group could significantly reduce the carrying rate of Campylobacter in broiler production without affecting the slaughter index. These results provided theoretical foundation for the prevention and control of Campylobacter contamination and the rational utilization of bacteriophages in broilers.

Key words: broiler; Campylobacter; bacteriophage; screening; application

CLC Number:

S858.31

XI Li, QIN Xinxi, SUN Xinfeng, SONG Yumin, LI Zhiqiang. Screening and Application of Campylobacter Bacteriophage in Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3273-3282.

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Screening and Application of Campylobacter Bacteriophage in Broilers

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