黄芪多糖对巨噬细胞天然免疫调节的研究进展

doi:10.16431/j.cnki.1671-7236.2022.05.016

Abstract

Abstract: As the first immune defense barrier of body,innate immune system plays a key role in the process of resisting pathogen infection.Maximize inducing the body’s protective mechanism to reduce the inflammatory damage caused by infection through stimulating the natural immune defense function of livestock and poultry by drugs or feed additives,has become one of the sustainable solutions to cope with the rampant pathogenic microorganisms and anti-microbial resistance in the current breeding environment.Being a class of biological macromolecules,Astragalus polysaccharides (APS) is one of the most immunologically active substance,and capable of various biological effects such as immune regulation,anti-virus,anti-stress,anti-oxidation,anti-tumor,etc.In clinical application,it can effectively improve the natural immune defense function of young animals and enhance the protective efficacy and growth performance of various vaccines.Therefore,the study of its regulation effect on the natural immune system of the body and its mechanism has become a research hotspot in recent years.However,due to the influence of origin,extraction and purification methods and complex chemical structure,the further research and development of APS activity are limited to some extent.The author reviewed the progress on the extraction technology and monosaccharide components of APS,and focused on the research progress of its two-way regulation for macrophages which is the main effect or cell of innate immunity,including polarization,function,inflammatory factors expression and the research progress on the intervention of macrophage-related Toll-like receptors (TLRs) signaling pathways,and pointed out the deficiencies in current research,aiming to provide references for the in-depth study of APS immunomodulatory mechanisms and the scientific basis on the promotion and application of APS in livestock and poultry breeding.

Key words: Astragalus polysaccharide (APS); macrophages; extraction; polarization; phagocytosis; TLRs signaling pathways

CLC Number:

R392.12

LIU Qian, GUO Zhiting, ZHANG Kang, WANG Xuezhi, ZHANG Jingyan, LI Jianxi. Research Progress on Astragalus Polysaccharide on Innate Immunalregulation of Macrophage[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1746-1756.

References

[1] 石丽霞,李科,秦雪梅.黄芪多糖双向抗肿瘤作用机制的研究进展[J].山西中医药大学学报,2021,22(2):145-149. SHI L X,LI K,QIN X M.Research progress on bidirectional antitumor mechanism of Astragalus polysaccharide[J].Journal of Shanxi University of Traditional Chinese Medicine,2021,22(2):145-149.(in Chinese)
[2] LI W F.Cytotoxicity and growth-inhibiting activity of Astragalus polysaccharides against breast cancer via the regulation of EGFR and ANXA1[J].Journal of Natural Medicines,2021,75(4):854-870.
[3] 李冬方,何小丽,都基莎,等.黄芪多糖心血管药理作用研究概况[J].辽宁中医杂志,2013,40(3):586-587. LI D F,HE X L,DU J S,et al.Study survey of Astragalus polysaccharides in angiomyocardiac pharmacologic action[J].Liaoning Journal of Traditional Chinese Medicine,2013,40(3):586-587.(in Chinese)
[4] 张熙,郑俊威,潘雪莲,等.黄芪多糖通过抑制炎性因子改善糖尿病大鼠肾损伤[J].湖北医药学院学报,2020,39(5):438-442. ZHANG X,ZHENG J W,PAN X L,et al.Astragalus polysaccharide improves kidney injury in diabetic rats by inhibiting inflammatory factors[J].Journal of Hubei Medical College,2020,39(5):438-442.(in Chinese)
[5] 禹博威,潘晓琼,陈君第霞,等.黄芪多糖对糖尿病动脉粥样硬化大鼠糖脂代谢的影响及血管内皮保护机制[J].浙江中医药大学学报,2021,45(5):447-453. YU B W,PAN X Q,CHEN J D X,et al.Effect of Astragalus polysaccharides on glucose and lipid metabolism in diabetic atherosclerosis rats and vascular endothelial protection mechanism[J].Journal of Zhejiang University of Traditional Chinese Medicine,2021,45(5):447-453.(in Chinese)
[6] 杜雪洋,梁建庆,何建成,等.黄芪多糖的药理作用研究[J].西部中医药,2019,32(6):152-155. DU X Y,LIANG J Q,HE J C,et al.Research progress of pharmacological action of Astragalus polysaccharides[J].Western Journal of Traditional Chinese Medicine,,2019,32(6):152-155.(in Chinese)
[7] 王佳敏,王延东,梁锐,等.日粮中添加黄芪复方颗粒对肉鸡生长、免疫及抗氧化功能的影响[J].中国畜牧杂志,2020,56(8):199-203. WANG J M,WANG Y D,LIANG R,et al.Effects of Astragalus compound granules on growth,immunity and antioxidant function of broilers[J].China Journal of Animal Husbandry,2020,56(8):199-203.(in Chinese)
[8] 向双云,周珍辉,曹金元,等.黄芪多糖对雏鸡生产性能及新城疫免疫性能的影响[J].饲料研究,2018,2:71-74. XIANG S Y,ZHOU Z H,CAO J Y,et al.Effects of Astragalus polysaccharides on production performance and Newcastle disease immunity of chicks[J].Feed Research,2018,2:71-74.(in Chinese)
[9] 魏炳栋,于维,陶浩,等.黄芪多糖对1~14日龄肉仔鸡生长性能、脏器指数及抗氧化能力的影响[J].动物营养学报,2011,23(3):486-491. WEI B D,YU W,TAO H,et al.Effects of Astragalus polysaccharides on growth performance,organ index and antioxidant capacity of 1-14 day old broilers[J].Chinese Journal of Animal Nutrition,2011,23(3):486-491.(in Chinese)
[10] KALLON S,LI X R,JI J,et al.Astragalus polysaccharide enhances immunity and inhibits H9N2 Avian influenza virus in vitro and in vivo[J].Journal of Animal Science and Biotechnology,2013,4(4):325-335.
[11] 牟鲁霞,祝路,赵爱华,等.黄芪多糖的提取及精制工艺研究[J].中药材,2009,32(11):1741-1745. MOU L X,ZHU L,ZHAO A H,et al.Study on extraction and purification from Astragalus polysaccharide[J].Journal of Chinese Medicinal Materials,,2009,32(11):1741-1745.(in Chinese)
[12] 权彦,何建军,刘靖丽,等.水提醇沉法提取黄芪中黄芪多糖的工艺优化及含量测定[J].当代化工,2018,47(7):1374-1376. QUAN Y,HE J J,LIU J L,et al.Optimization of extraction process of Astragalus polysaccharides from Astragalus membranaceus by water extraction and ethanol precipitation and determination of Astragalus polysaccharides content[J].Contemporary Chemical Industry,2018,47(7):1374-1376.(in Chinese)
[13] 余萍,赵宝娥,李欠.黄芪多糖不同提取优化工艺比较[J].世界中医药,2021,3(6):1-9. YU P,ZHAO B E,LI Q.Comparison of different extraction optimization processes of Astragalus polysaccharides[J].World Chinese Medicine,2021,3(6):1-9.(in Chinese)
[14] 韩真贤,张宇,张云杰,等.黄芪多糖三种提取工艺比较[J].黑龙江医药科学,2009,32(4):72-73. HAN Z X,ZHANG Y,ZHANG Y J,et al.Comparison of three extraction processes for Astragalus polysaccharide[J].Heilongjiang Medicine and Pharmacy,2009,32(4):72-73.(in Chinese)
[15] 刘永录,张国祖,樊克锋,等.黄芪多糖的提取和纯化方法研究[J].河南农业科学,2010,4(6):141-143. LIU Y L,ZHANG G Z,FAN K F,et al.Study on the technologies for extraction and purification of Astragalus polysaccharides[J].Henan Agricultural Science,2010,4(6):141-143.(in Chinese)
[16] 田洛,宣依昉,范荣军,等.醇碱提取法提取黄芪多糖[J].吉林大学学报(理学版),2006,4(4):652-657. TIAN L,XUAN Y F,FAN R J,et al.Ethanol-alkali extraction method of Astragalus polysaccharides[J].Journal of Jilin University (Science Edition),2006,4(4):652-657.(in Chinese)
[17] 张曼,韩飞,王晶钰.黄芪多糖微波提取工艺研究进展[J].陕西农业科学,2015,61(3):85-88. ZHANG M,HAN F,WANG J Y.Research progress on microwave extraction of Astragalus polysaccharides[J].Shaanxi Journal of Agricultural Sciences,2015,61(3):85-88.(in Chinese)
[18] 梁子敬,张景艳,冯海鹏,等.响应面优化黄芪多糖的提取工艺[J].食品研究与开发,2018,39(21):72-76. LIANG Z J,ZHANG J Y,FENG H P,et al.Optimization of extraction technology for polysaccharides of Astragalus with response surface methodology[J].Food Research and Development,2018,39(21):72-76.(in Chinese)
[19] 秦哲.黄芪发酵后主要有效成分变化分析及多糖对大鼠实验性肝纤维化影响[D].兰州:甘肃农业大学,2012. QIN Z.Changes of main active components after Astragalus membranaceus fermentation and the effect of polysaccharide on experimental liver fibrosis in rats[D].Lanzhou:Gansu Agricultural University,2012.(in Chinese)
[20] 胡媛媛,吕瑞红,纪永升,等.黄芪多糖的脱蛋白工艺考察[J].中国实验方剂学杂志,2017,23(20):25-29. HU Y Y,LYU R H,JI Y S,et al.Investigation of deproteinization of Astragalus polysaccharide[J].Chinese Journal of Experimental Traditional Medical Formulae,2017,23(20):25-29.(in Chinese)
[21] 芮雯,李婵艺,陈宏远.黄芪多糖的结构表征与生物活性研究进展[J].中药新药与临床药理,2019,30(2):264-270. RUI W,LI C Y,CHEN H Y.Progress of structure characterization and biological activities of Astragalus polysaccharides[J].Traditional Chinese Drug Research & Clinical Pharmacology,2019,30(2):264-270.(in Chinese)
[22] 刘卫东,王兰英,任建国,等.黄芪多糖的化学组成和提取方法研究进展[J].西北药学杂志,2010,25(2):151-153. LIU W D,WANG L Y,REN J G,et al.Research progress on chemical composition and extraction methods of Astragalus polysaccharides[J].Northwest Pharmaceutical Journal,2010,25(2):151-153.(in Chinese)
[23] 陈艳蕊,毛欣月,金文闻,等.黄芪多糖结构及其单糖组成的气相色谱-质谱研究[J].现代生物医学进展,2011,11(1):4632-4635. CEHN Y R,MAO X Y,JIN W W,et al.Study on Astragalus polysaccharide structure and monosaccharide constituents by gas chromatography-mass spectro-metry[J].Progress in Modern Biomedicine,2011,11(1):4632-4635.(in Chinese)
[24] 唐雨薇,张宇,王宇亮,等.黄芪多糖分离与结构特征分析[J].时珍国医国药,2014,25(5):1097-1100. TANG Y W,ZHANG Y,WANG Y L,et al.Isolation and structural feature analysis of Astragalus polysaccharides[J].Lishizhen Medicine and Materia Medica Research,2014,25(5):1097-1100.(in Chinese)
[25] JIANG Y P,QI X H,GAO K,et al.Relationship between molecular weight,monosaccharide composition and immunobiological activity of Astragalus polysaccharides[J].Glycocon Jugate Journal,2016,33(5):755-761.
[26] 周宪宾,姚成芳.巨噬细胞M1/M2极化分型的研究进展[J].中国免疫学杂志,2012,28(10):957-960. ZHOU X B,YAO C F.Progress in M1/M2 polarization typing of macrophages[J].Chinese Journal of Immunology,2012,28(10):957-960.(in Chinese)
[27] 吴秀华,郑文洁.巨噬细胞极化[J].中华临床免疫和变态反应杂志,2017,11(2):161-165. WU X H,ZHENG W J.Research progress of macrophage polarization[J].Chinese Journal of Allergy & Clinical Immunology,2017,11(2):161-165.(in Chinese)
[28] DEREK R,MANISHA T,MANUEL P J.Biological effects of nanoparticles on macrophage polarization in the tumor microenvironment[J].Nanotheranostics,2019,3(1):66-88.
[29] WEI W,LI Z P,BIAN Z X,et al.Astragalus polysaccharide RAP induces macrophage phenotype polarization to M1 via the Notch signaling pathway[J].Molecules (Basel,Switzerland),2019,24(10):2016.
[30] BAMODU O A,KUO K T,WANG C H,et al.Astragalus polysaccharides (PG₂) enhances the M1 polarization of macrophages,functional maturation of dendritic cells,and T cell-mediated anticancer immune responses in patients with lung cancer[J].Nutrients,2019,11(10):2264.
[31] 柴长斌,马茜,周丽,等.黄芪多糖可提升小鼠巨噬细胞和自然杀伤细胞的抗肿瘤作用[J].中华微生物学和免疫学杂志,2019,4(4):292-297. CHAI C B,MA X,ZHOU L,et al.Astragalus polysaccharide improves anti-tumor immunity mediated by macrophages and natural killer cells in mice[J].Chinese Journal of Microbiology and Immunology,2019,4(4):292-297.(in Chinese)
[32] 刘建春,张红珍,李俊莲,等.黄芪多糖对实验性自身免疫性脑脊髓炎小鼠脾细胞的免疫调节作用研究[J].中国免疫学杂志,2021,37(14):1701-1705. LIU J C,ZHANG H Z,LI J L,et al.Immunoregulatory effect of Astragalus polysaccharide on spleen cells of experimental autoimmune encephalomyelitis mice[J].Chinese Journal of Immunology,2021,37(14):1701-1705.(in Chinese)
[33] 郑燕,王娜,刘静乔,等.黄芪多糖对LPS诱导流产大鼠子宫巨噬细胞的影响及机制研究[J].中国免疫学杂志,2018,34(8):1174-1177. ZHENG Y,WANG N,LIU J Q,et al.Effect of Astragalus polysaccharides on uterine macrophages of LPS induced abortion rats and its mechanism[J].Chinese Journal of Immunology,2018,34(8):1174-1177.(in Chinese)
[34] 宁康健,阮祥春,吕锦芳,等.黄芪对小鼠腹腔巨噬细胞吞噬能力的影响[J].中国中药杂志,2005,30(21):1670-1672. NING K J,RUAN X C,LYU J F,et al.Effects of Huangqi on phagocytic activity of peritoneal macrophage of mice[J].China Journal of Chinese Materia Medica,2005,30(21):1670-1672.(in Chinese)
[35] 刘印华,赵志强,李树义,等.黄芪多糖对免疫功能影响的体内实验研究[J].河北医药,2015,37(4):485-487. LIU Y H,ZHAO Z Q,LI S Y,et al.Effects of Astragalin polysaccharides on immune function in vivo[J].Hebei Medical Journal,2015,37(4):485-487.(in Chinese)
[36] 史晶晶,时博,苗明三.黄芪多糖对环磷酰胺致免疫抑制小鼠免疫功能的影响[J].中医学报,2016,31(2):243-246. SHI J J,SHI B,MIAO M S.Effects of Astragalus polysaccharides on immunological function of the immunosuppressional model mice caused by cyclophosphamide[J].ACTA Chinese Medicine,2016,31(2):243-246.(in Chinese)
[37] QIU H,CHENG G,XU J,et al.Effects of Astragalus polysaccharides on associated immune cells and cytokines in immunosuppressive dogs[J].Procidia in Vaccinology,2010,2(1):26-33
[38] YANG B,XIAO B,SUN T Y.Antitumor and immunomodulatory activity of Astragalus membranaceus polysaccharides in H22 tumor-bearing mice[J].International Journal of Biological Macromolecules,2013,62(8):287-290.
[39] 张雷芳.黄芪多糖对布鲁氏菌感染巨噬细胞的调节作用[D].保定:河北大学,2013. ZHANG L F.Regulation of Astragalus polysaccharides on macrophages infected by Brucella[D].Baoding:Hebei University,2013.(in Chinese)
[40] ZHANG N,LI J,HU Y,et al.Effects of Astragalus polysaccharide on the immune response to foot-and-mouth disease vaccine in mice[J].Carbohydrate Polymers,2010,82(3):680-686.
[41] WEI W,XIAO H T,BAO W R,et al.TLR-4 may mediate signaling pathways of Astragalus polysaccharide (RAP) induced cytokine expression of RAW264.7 cells[J].Journal of Ethnopharmacology,2016,179:243-252.
[42] WANG Z,LIU Z,ZHOU L,et al.Immunomodulatory effect of APS and PSP is mediated by Ca²⁺-cAMP and TLR4/NF-κB signaling pathway in macrophages[J].International Journal of Biological Macromolecules,2017,94(Pt A):283-289.
[43] FENG S B.Astragalus polysaccharide enhances the immune function of RAW264.7 macrophages via the NF-κB p65/MAPK signaling pathway[J].Experimental and Therapeutic Medicine,2021,21(1):20.
[44] ZHOU L J,LIU Z J,WANG Z X,et al.Astragalus polysaccharides exerts immunomodulatory effects via TLR4-mediated MyD88-dependent signaling pathway in vitro and in vivo[J].Scientific Reports,2017,7:44822.
[45] 孟婷婷.黄芪三种成分对小鼠巨噬细胞RAW264.7的免疫增强作用及其机制研究[D].合肥:安徽农业大学,2016. MENG T T.Immune enhancement effect and its mechanism of three components of Mongolian milkvetch root in macrophage RAW264.7 of mice[D].Hefei:Anhui Agricultural University,2016.(in Chinese)
[46] 曹丽萍,丁炜东,张柳,等.香菇多糖和黄芪多糖对鲤免疫细胞的活性和IL-1β体外诱生表达的影响[J].水产学报,2008,4(4):628-635. CAO L P,DING W D,ZHANG L,et al.Effects of the activation of immunological cells and the expression of interleukin-1β in the cyprinuscarpio after stimulation by Lentinan and Astragalus polysaccharides[J].Journal of Fisheries of China,2008,4(4):628-635.(in Chinese)
[47] 孙林浩,程安怡,黄小红,等.黄芪多糖对大黄鱼头肾巨噬细胞的免疫调节作用[J].应用海洋学学报,2020,39(1):27-34. SUN L H,CHENG A Y,HUANG X H,et al.Immunoregulation of Astragalus polysaccharide on macrophages from head kidney of large Yellow croaker[J].Journal of Applied Oceanography,2020,39(1):27-34.(in Chinese)
[48] 周丽菁,汪志学,龙婷婷,等.黄芪多糖在体外巨噬细胞-乳腺癌细胞共培养体系中的作用[J].免疫学杂志,2017,33(6):469-476. ZHOU L J,WANG Z X,LONG T T,et al.Roles of Astragalus polysaccharides in coculture system of breast cancer cells and macrophages in vitro[J].Immunology Journal,2017,33(6):469-476.(in Chinese)
[49] XU H D,YOU C G,ZHANG R L,et al.Effects of Astragalus polysaccharides and astragalosides on the phagocytosis of mycobacterium tuberculos is by macrophages[J].Journal of International Medical Research,2007,35(1):84-90.
[50] 韩乾杰.植物多糖对仔猪生长性能、免疫功能和肠道健康的影响研究[D].杭州:浙江农林大学,2019. HAN Q J.Effects of plant polysaccharides on growth performance,immune function and intestinal health in weaned piglets[D].Hangzhou:Zhejiang A&F University,2019.(in Chinese)
[51] 陈广勇,韩乾杰,张玲玲,等.黄芪多糖对脂多糖刺激小鼠巨噬细胞形态及免疫功能的影响[J].动物营养学报,2020,32(9):4358-4365. CHEN G Y,HAN Q J,ZHANG L L,et al.Effects of Astragalus polysaccharides on morphology and immune function of macrophages stimulated by lipopolysaccharide in mice[J].Chinese Journal of Animal Nutrition,2020,32(9):4358-4365.(in Chinese)
[52] 景晓平,裴明,何丽.基于抗体芯片技术的黄芪多糖及板蓝根多糖免疫调节机制研究[J].中华中医药杂志,2013,28(11):3420-3423. JING X P,PEI M,HE L,et al.Research immunomodulatory mechanism of Astragalus polysaccharide and isatis root polysaccharide based on antibody chip technology[J].Chinese Journal of Traditional Chinese Medicine,2013,28(11):3420-3423.(in Chinese)
[53] 李冬方,顾宁,黄伟.黄芪多糖对THP-1巨噬细胞HSP60诱导下IL-6、TNF-α表达的影响[J].时珍国医国药,2015,26(12):2830-2832. LI D F,GU N,HUANG W.Influence of Astragalus polysaccharide on the expression of IL-6 and TNF-α of the HSP60-induced THP-1 macrophages[J].Lishizheng Medicine and Materia Medica Research,2015,26(12):2830-2832.(in Chinese)
[54] 徐荔,何小鹃,柴旺,等.黄芪多糖对内毒素诱导巨噬细胞产生TNF-α、IL-1β及NO的影响[J].中国中医基础医学杂志,2011,17(5):503-504. HE L,HE X J,CHAI W,et al.Effect of Astragalus polysaccharide on TNF production by macrophages induced by endotoxin-α,IL-1β and NO[J].Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2011,17(5):503-504.(in Chinese)
[55] LEE K Y,JEON Y J.Macrophage activation by polysaccharide isolated from Astragalus membranaceus[J].International Immunopharmacology,2005,5(7-8):1225-1233.
[56] 赵鲁杭.黄芪多糖的制备及其对巨噬细胞和树突状细胞免疫功能的影响[D].杭州:浙江大学,2011. ZHAO L H.Preparation of Astragalus polysaccharide and its effect on immune function of macrophages and dendritic cells[D].Hangzhou:Zhejiang University,2011.(in Chinese)
[57] 汪志学.Ca(²⁺)-cAMP信号通路介导的云芝糖肽和黄芪多糖免疫调节机制的研究[D].重庆:重庆医科大学,2016. WANG Z X.Immunomodulatory mechanism of coriolus versicolor glycopeptide and Astragalus polysaccharides mediated by Ca(²⁺)-cAMP signaling pathway[D].Chongqing:Chongqing Medical University,2016.(in Chinese)
[58] QIU Y,HU Y L,CUI B A,et al.Immunopotentiating effects of four Chinese herbal polysaccharides administered at vaccination in chickens[J].Poultry Science,2007,86(12):2530-2535.
[59] 路德成.黄芪多糖对新城疫疫苗免疫效果的影响[J].养殖与饲料,2019,1:39-40. LU D C.Effect of Astragalus polysaccharide on immune effect of Newcastle disease vaccine[J].Breeding and Feed,2019,1:39-40.(in Chinese)
[60] 蒋广志.黄芪多糖对蓝耳弱毒苗免疫调节及抗蓝耳病毒感染的研究[D].合肥:安徽农业大学,2015. JIANG G Z.Effect of Astragalus polysaccharide on immune regulation of blue ear attenuated vaccine and anti blue ear virus infection[D].Hefei:Anhui Agricultural University,2015.(in Chinese)
[61] WU S J.Effect of dietary Astragalus membranaceus polysaccharide on the growth performance and immunity of juvenile broilers[J].Poultry Science,2018,97(10):3489-3493.
[62] CHEN H L,LI D F,CHANG B Y,et al.Effects of Chinese herbal polysaccharides on the immunity and growth performance of young broilers[J].Poultry Science,2003,82(3):364-370.
[63] 徐松琪.黄芪多糖和葛根提取物对哺乳母猪生产性能及血清生化免疫指标的影响[D].哈尔滨:东北农业大学,2018. XU S Q.Effects of Astragalus polysaccharide and pueraria extract on performance and serum biochemical immune indexes of lactating sows[D].Harbin:Northeast Agricultural University,2018.(in Chinese)
[64] BARJESTEH N,BEHBOUDI S,BRISBIN J T,et al.TLR ligands induce antiviral responses in chicken macrophages[J].PLoS One,2017,9(8):e105713.

Research Progress on Astragalus Polysaccharide on Innate Immunalregulation of Macrophage

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[3]	PENG Zhiping, ZHANG Jingyan, GUO Zhiting, WANG Lei, ZHANG Kai, ZHANG Kang, WANG Guibo, QIU Zhengying, WANG Xuezhi, LI Jianxi. Extraction and Content Detection of Cordycepin from Cordyceps militaris Residual Medium by Hydrothermal Reflux Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3333-3342.
[4]	LI Yipeng, ZHANG Hu, REN Juan, REN Xin, LIU Juxiang, WANG Gengnan. Study on the Extraction Process of Chicoric Acid from Echinacea Root and Its Protective Effects on Oxidative Stress Damaged Intestinal Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2497-2505.
[5]	LI Yahui, LUO Shengjun, TANG Xinggang, YUAN Minggui, WEI Qilin, LOU Hua, XIANG Rong. Study on Optimization of Extraction Process and Antibacterial Activity to Clostridium Perfringens of Total Flavonoids from Vitex negundo L.var. cannabifolia (Sieb.et Zucc.) Hand.-Mazz. [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2406-2413.
[6]	CHEN Yuxiao, LI Huanjuan, XI Xiaohua, HAO Jie, ZHOU Degang, SI Hongbin. Study on Extraction Process of Jiawei Pingwei Oral Liquid [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2414-2422.
[7]	QIU Shulei, CHEN Xiaolan, YANG Haifeng, CHEN Yuku, HU Yuanliang, GAO Yongxu, WU Caihong. Effects of Selenized Garlic Polysaccharide on the Function of Mouse Peritoneal Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1144-1152.
[8]	YANG Yu, GUAN Wenchao, CUI Xiaozhen, JIA Yongxin, YANG Shiyu. Effects of Licochalcone A on Oxidative Stress Induced by Haemophilus parasuis Infection in Porcine Alveolar Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 480-487.
[9]	XIE Shanshan, YANG Qin, DENG Xiaoyu, YI Jihai, WANG Zhen, CHEN Chuangfu. Effects of Mitochondrial Fusion Protein 2 Gene Interference and Overexpression on Macrophage Apoptosis Induced by Brucella [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 283-293.
[10]	YU Jiamin, ZHAO Qian, ZHANG Zhiyan, XU Haiyan, GU Wei. Separation,Purification and Characteristics Analysis of Antibacterial Peptides from Paenibacillus polymyxa [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2830-2837.
[11]	ZHANG Jing, QIAN Yinghong, ZHANG Kai, WU Jindi, LIU Bo, MAO Wei, CAO Jinshan. Effects of Staphylococcus aureus Lipoprotein on Immunity in M1 Mouse Bone Marrow-Derived Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3079-3086.
[12]	HE Weizhao, ZHANG Huiyan, WANG Hao, ZHAO Qingyu, TANG Chaohua, ZHANG Junmin. Extraction Methods of Yolk Antibody and Its Application in Prevention and Treatment of Bacterial Intestinal Diseases in Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 640-649.
[13]	LIU Haiyao, LI Na, ZHANG Xiaoguang, LI Ziheng, BAO Chuntong, JIANG Hexiang, LIU Baijun, XIAO Jiameng, WANG Jun, LI Fengyang, LEI Liancheng. Role of S100A12 in the Expression of Inflammatory Cytokines and Phagocytosis in Porcine Alveolar Macrophages Induced by Actinobacillus pleuropneumoniae [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4262-4273.
[14]	XIE Zongxiu, LI Shangwei, ZHOU Deshun, SONG Xiangdong, JIA Ning, LIANG Jianping. Study on Essential Oil Composition Analysis and Antibacterial Activity from Dicranostigma leptopodum (Maxim.) Fedde [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1611-1617.
[15]	MAO Xin, ZHANG Guizhen, QU Jinyao, ZHANG Yuhao, LU Guozhu, GAO Yonglin, LI Yanshen. Discovery,Isolation,Identification and Characterization of Novel Non-ribosomal Peptide Antibacterial Active Substances in Bacillus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4093-4102.