摩拉水牛PRKAA2基因多态性与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2020.03.017

Abstract

Abstract: This study was aimed to investigate the genetic polymorphisms of PRKAA2 gene in Murrah buffalo and its associations with growth traits,which could help to mine significant genetic markers for molecular marker assisted selection of Murrah buffalo.SNPs located in exon 4 and intron 3 were detected in PRKAA2 gene of Murrah buffalo by random DNA sequencing method.Genetic variation of SNPs and the association of different genotypes with growth traits of Murrah buffalo were analyzed by bioinformatics method and statistical software.The results showed that 4 SNPs were detected in PRKAA2 gene,namely c.462 G>A,IVS3.557 T>C,IVS3.560 C>T and IVS3.565 G>A,respectively,there were 3 genotypes.The fitness test showed that PRKAA2 gene on polymorphic loci in Hardy-Weinberg equilibrium.All of 4 SNPs were moderate polymorphism,and could be constructed 3 haplotypes,T-C-G-G type was the dominant haplotype in Murrah buffalo.There was a complete linkage disequilibrium between IVS3.560 C>T and c.462 G>A.Association analysis results indicated that H2 haplotype was significantly correlated with body length (BL)and hip width (HW) in Murrah buffaloes (P<0.05).c.462 G>A was significantly correlated with the withers height (WH),height at hip cross (HHC),rump length (RL),pin bone width (PBW) and HW of Murrah buffalo,WH and HHC of GG and AA genotypes were significantly higher than that of GA genotype,RL of GG genotype were significantly higher than that of GA and AA genotypes,and PBW and HW of AA genotype were significantly higher than that of GA genotypes (P<0.05).There was no significant correlation between IVS3.557 T>C and body size traits of Murrah buffalo (P>0.05).IVS3.560 C>T was significantly correlated with WH,HHC and RL of Murrah buffalo,WH and HHC of CC and TT genotypes were significantly higher than that of CT genotype (P<0.05),and RL of CC genotype was significantly higher than that of CT genotype (P<0.05).IVS3.565 G>A was significantly correlated with BL of Murrah buffalo,BL of GG and GA genotypes were significantly higher than that of AA genotype (P<0.05).In conclusion,3 SNPs (c.462 G>A,IVS3.560 C>T and IVS3.565 G>A) of PRKAA2 gene had potential effects on growth traits of Murrah buffalo, and could be used for candidate gene and marker-assisted selection in selection of new varieties of Murrah buffalo.

Key words: Murrah buffalo; PRKAA2 gene; SNPs; growth traits

CLC Number:

S813.3

ZHENG Haiying, YANG Chunyan, ZHENG Wei, YU Nongqi, WEN Chongli, WEI Kelong, LI Lingyu, HUANG Chunli, HUANG Jiaxiang, SHANG Jianghua. Association Analysis Between PRKAA2 Gene Polymorphisms and Growth Traits in Murrah Buffalo[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 789-798.

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Association Analysis Between PRKAA2 Gene Polymorphisms and Growth Traits in Murrah Buffalo

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