基于SRY基因分析梅花鹿的遗传多样性及父系类型

doi:10.16431/j.cnki.1671-7236.2019.02.019

Abstract

Abstract:

This study was aimed to analyze the genetic diversity and paternal typec of sika deer based on SRY gene.144 individuals from five subspecies,such as Cervus nippon hortulorum,Cervus nippon yesoensis,Cervus nippon centralis,Cervus nippon nippon and Cervus nippon yakushimae,were selected for DNA extraction using kit and traditional phenol/imitation (1:1) methods.PCR amplification and PCR products sequence were used to analyze the nucleotide diversity (Pi),haplotype diversity (Hd),genetic distance and phylogenetic relationship of sika deer.The results showed that 6 SNPs polymorphisms were detected at positions 47,62,602,1 148,1 569 and 1 604 bp in the 1 613 bp sequences,which accounted for 0.3% of the total number of nucleotides.The base substitution was dominated by base conversion.6 haplotypes of Hap-1,Hap-2,Hap-3,Hap-4,Hap-5 and Hap-6 were identified by 6 SNPs polymorphisms,Hap-4,Hap-5 and Hap-6 were the new haplotypes.The sequence of nucleotide diversity from high to low were:Cervus nippon nippon, Cervus nippon yakushimae,Cervus nippon hortulorum,Cervus nippon centralis,Cervus nippon yesoensis.The smallest genetic distance among subspecies was 0.000056 between Cervus nippon yesoensis and Cervus nippon centralis,and the largest distance was 0.001733 between Cervus nippon hortulorum and Cervus nippon nippon.Phylogenetic trees were constructed based on haplotypes and all individuals using the adjacency method.The phylogenetic tree showed that compared with the Japanese sika deer,Cervus nipppon hortulorum was closer to Cervus elaphus,Cervus nippon hortulorum had two branches,and Japanese sika deer had no obvious branches,and the other haplotypes were evolved by Hap-3.The haplotype minimum span network map was consistent with the evolutionary tree.This results indicated that there were two patrilineal types in Cervus nippon hortulorum,there was a paternal type in Japanese sika deer,Hap-3 was the original haplotype of Japanese sika deer.

Key words: sika deer; SRY gene; genetic diversity; paternal type

CLC Number:

S825

DONG Yimeng, LIU Huamiao, Hidetoshi B. Tamate, LIU Huitao, JU Yan, XING Xiumei, HE Jinming, JU Guichun. Analysis of Genetic Diversity and Paternal Type of Sika Deer Based on SRY Gene[J]. , 2019, 46(2): 489-496.

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Analysis of Genetic Diversity and Paternal Type of Sika Deer Based on SRY Gene

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