China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (1): 28-36.doi: 10.16431/j.cnki.1671-7236.2020.01.004

• Biotechnology • Previous Articles     Next Articles

Cloning and Bioinformatics Analysis of S100A16 Gene in Sika Deer

CHEN Jiqian, XIA Yanling, GAO Yueyu, LIU Weishi   

  1. College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China
  • Received:2019-07-19 Online:2020-01-20 Published:2020-01-17

Abstract: In order to explore the S100A16 gene sequence and biological characteristics of Sika deer (Cervus nippon),primers were designed according to the S100A16 gene sequence of cattle and sheep in GenBank database,and the cDNA of S100A16 gene in Sika deer was successfully obtained by RT-PCR and molecular cloning technology with the top tissue cDNA of sika deer antler as template.Bioinformatics analysis showed that the total length of CDS region of S100A16 gene in Sika deer was 312 bp,encoding 103 amino acids;the protein contained 11 phosphorylation sites,transmembrane domain,and no signal peptide,which was the stable protein that played a role in the cell;The protein only contained the EF-hand of S100 protein family in the C-terminal,which was composed of 12 amino acids,and the EF-hand at the N-terminal was composed of 15 amino acids.The second-order structure of S100A16 protein in Sika deer was mainly composed of alpha-helix and ranchorn coil;the third-order structure showed that the protein had two Ca2+ binding sites;the amino acid sequence of S100A16 protein in Sika deer had the highest homology with Eastern European red deer,which was 100%,and the phylogenetic tree was constructed with the amino acid sequence of S100A16 protein in some other species.S100A16 gene was relatively conservative in evolution and conformed to the characteristics of functional genes.The results provided the basis for further revealing the function and expression mechanism of S100A16 gene in Sika deer.

Key words: Sika deer; S100A16 gene; molecular cloning; bioinformatics analysis

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