内蒙古绒山羊KAP9.2基因遗传多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.11.017

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the relationship between insertion/deletion (InDels) mutation of KAP gene family keratin-associated protein 9.2 (KAP9.2) gene and growth traits of Inner Mongolia cashmere goat, in order to provide a theoretical basis for molecular breeding of goats.【Method】 A total of 606 female Inner Mongolia cashmere goats were selected as the research objects, the ear tissue was collected to extract DNA, and their body weight and body size, such as body height, length and chest circumference were measured.The InDel mutation of KAP9.2 gene was detected by agarose gel electrophoresis and direct sequencing technology, and genetic diversity indicators were detected.The association between mutation locus and growth traits was analyzed by SPSS 23.0 software.【Result】 There was a 30 bp InDelmutation in the exon region of the KAP9.2 gene in Inner Mongolia cashmere goats, which produced three genotypes of II, ID and DD.The polymorphism information content (PIC) showed that the locus was moderately polymorphic (0.25<PIC<0.50) in the breeding goat population, low polymorphism (PIC<0.25) in the adult goat population, and both were in Hardy-Weinberg equilibrium (P>0.05).In the whole population, the locus was low polymorphism (PIC<0.25), but deviated from Hardy-Weinberg equilibrium (P<0.05).The results of association analysis showed that in the breeding goat population, the 30 bp InDel mutation had significant effect on body length and height at hip cross (P<0.05), and had extremely significant effect on body height, chest depth and chest width (P<0.01).In the adult goat population, this mutation had extremely significant effect on cannon circumference, chest depth and chest width (P<0.01).Further analysis found that this mutation had significant effect on body length and height at hip cross (P<0.05), and had extremely significant effect on body height, chest depth and chest width (P<0.01) in the whole population.【Conclusion】 The InDel mutation of KAP9.2 gene had significant or extremely significant effect on many growth traits of Alashan-type Inner Mongolia cashmere goat, and could be used as a molecular marker for the breeding of excellent traits of Inner Mongolia cashmere goat.

Key words: KAP9.2 gene; Inner Mongolia cashmere goats; insertion/deletion (InDel); growth traits

CLC Number:

S823.8

WANG Congliang, FU Qi, JU Minying, BAI Jingjing, LI Xinshu, SONG Xiaoyue, LI Longping, LAN Xianyong, QU Lei, ZHANG Lei, ZHU Haijing. The Genetic Polymorphisms of KAP9.2 Gene and Its Correlation Analysis with Growth Traits of Inner Mongolia Cashmere Goats[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4270-4278.

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The Genetic Polymorphisms of KAP9.2 Gene and Its Correlation Analysis with Growth Traits of Inner Mongolia Cashmere Goats

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