延边黄牛UCP2、UCP3基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.10.020

Abstract

Abstract: The purpose of this study was to investigate the polymorphism of uncoupling protein 2 (UCP2) and uncoupling protein 3 (UCP3) genes and its association with growth traits in Yanbian Yellow cattle. 120 Yanbian Yellow cattle aged 24 months were used as experimental subjects, construction of mixed DNA pool after extracting blood genomic DNA. UCP2 and UCP3 genes were sequenced and analyzed by DNA sequencing method, the genotyping of UCP2 and UCP3 genes were detected by high resolution melting curve, and the correlation analysis were conducted with growth traits. The results showed that UCP2 gene at 53 412 568 bp had C>G mutation (g. 53412568 C>G) and two alleles of C and G, which formed three genotypes of CG(0.358), GG(0.125) and CC(0.516). UCP3 gene at 53 443 536 bp had C>T mutation (g. 53443536 C>T) and two alleles of C and T, which formed three genotypes of CT(0.383), CC(0.450) and TT(0.167). Correlation analysis showed that the body weight and hip width of CC genotype of UCP2 gene g. 53412568 C>G were significantly higher than those of GG genotype, and the back height of CG genotype was significantly higher than that of GG genotype (P<0.05). The chest circumference of CC genotype individuals of UCP3 gene g. 53443536 C>T was significantly higher than that of TT genotype (P<0.05). In conclusion, UCP2 and UCP3 genes played a certain role in the growth and development of Yanbian Yellow cattle, which could be used as a reference for the research of modern molecular breeding of Yanbian Yellow cattle in the future.

Key words: Yanbian Yellow cattle; UCP2 gene; UCP3 gene; SNP; growth traits

CLC Number:

S813.3

ZHAO Yi, WANG Sihan, LIU Lei, TIAN Quan, LIU Haixing, LI Zhongshu, ZHANG Junzheng, JI Shuang. Polymorphism of UCP2 and UCP3 Genes and Its Association with Growth Traits in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3691-3698.

References

[1] 李敬, 李娜, 孙宝忠, 等.中国五大良种黄牛品质特性的研究进展[J]. 肉类研究, 2015, 29(9):34-37. LI J, LI N, SUN B Z, et al.Recent progress in the study of meat quality characteristics of five dominant cattle breeds[J]. Meat Research, 2015, 29(9):34-37.(in Chinese)
[2] 郜攀.UCP4和BMCP-1在大鼠缺氧时脑组织线粒体能量合成与呼吸氧耗改变中的作用[D].重庆:第三军医大学, 2006. GAO P.The role of UCP4 and BMCP-1 in the changes of brain mitochondrial energy synthesis and respiratory oxygen consumption during hypoxia in rats[D].Chongqing:Third Military Medical University, 2006.(in Chinese)
[3] 李海.线粒体解耦联蛋白2与能量代谢[J]. 内江师范学院学报, 2010, 25(8):52-55. LI H.UCP2 and energy metabolism[J]. Journal of Neijiang Teachers College, 2010, 25(8):52-55.(in Chinese)
[4] WANG X X, SUN B F, JING Q, et al.mRNA m6A plays opposite role in regulating UCP2 and PNPLA2 protein expression in adipocytes[J]. International Journal of Obesity, 2018, 42(11):1912-1924.
[5] LOMAX T M, ASHRAF S, YILMAZ G, et al.Loss of uncoupling protein 3 attenuates Western diet-induced obesity, systemic inflammation, and insulin resistance in rats[J]. Obesity, 2020, 28(9):1687-1697.
[6] PASCUALGAMARRA J M, SALAZARTORTOSA D F, LABAYEN I, et al.Association of UCP1, UCP2 and UCP3 gene polymorphisms with cardiovascular disease risk factors in European adolescents:The HELENA study[J]. Pediatric Research, 2020, 88(2):265-270.
[7] MAYARA S, JAKELINE R, MILENE M, et al.UCP2, IL18, and miR-133a-3p are dysregulated in subcutaneous adipose tissue of patients with obesity[J]. Molecular and Cellular Endocrinology, 2020, 509:110805.
[8] LUO L, HUANG P, WANG T T, et al.Associations of maternal diabetes mellitus and adiponectin gene polymorphisms with congenital heart disease in offspring:A case-control study[J]. Medicine, 2021, 100(9):e24672.
[9] 周晶, 郑银, 段英连, 等.解耦联蛋白3基因-55 C/T多态性与2型糖尿病血糖控制的相关性[J]. 山西医科大学学报, 2019, 50(5):652-657. ZHOU J, ZHENG Y, DUAN Y L, et al.Effect of -55 C/T polymorphism of UCP3 on glycemic control in type 2 diabetes mellitus[J]. Journal of Shanxi Medical University, 2019, 50(5):652-657.(in Chinese)
[10] 张继, 吴雪, 邱淦远, 等.江口萝卜猪UCP2基因多态性与基因表达量及肉品质的相关性研究[J]. 中国畜牧杂志, 2019, 55(10):71-77. ZHANG J, WU X, QIU G Y, et al.Correlation between UCP2 gene polymorphism and gene expression and meat quality in Jiangkou Luobo pig[J]. Chinese Journal of Animal Husbandry, 2019, 55(10):71-77.(in Chinese)
[11] 张继, 吴雪, 邱淦远, 等.江口萝卜猪UCP3基因变异及其表达量与肉质性状关联性分析[J]. 农业生物技术学报, 2020, 28(3):490-500. ZHANG J, WU X, QIU G Y, et al.et al.Correlation analysis of UCP3 gene variation, expression and meat quality traits in Jiangkou Luobo pig(Sus scrofa)[J]. Journal of Agricultural Biotechnology, 2020, 28(3):490-500.(in Chinese)
[12] 李威, 贺江燕, 翟刚, 等.低温处理对异育银鲫腹部脂肪组织能量代谢相关基因表达的影响[J]. 生物资源, 2020, 42(6):698-703. LI W, HE J Y, ZHAI G, et al.Effect of cold treatment on the expression of genes related to energy metabolism in abdominal adipose tissue of gible carp[J]. Biological Resources, 2020, 42(6):698-703.(in Chinese)
[13] 蒋秋斐, 罗晓瑜, 洪龙, 等.宁夏西门塔尔杂种牛UCP2基因多态性及其与生长性状的关联性分析[J]. 中国畜牧兽医, 2016, 43(7):1843-1849. JIANG Q F, LUO X Y, HONG L, et al.Correlation analysis between UCP2 gene and growth traits in Ningxia hibrid Simmental cattle[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(7):1843-1849.(in Chinese)
[14] 蒋秋斐, 洪龙, 封元, 等.西门塔尔杂种牛UCP3基因多态性与生长性状的关联性分析[J]. 农业生物技术学报, 2018, 26(4):642-651. JIANG Q F, HONG L, FENG Y, et al.Polymorphism in UCP3 gene and its association with growth traits in hybrid Simmental cattle (Bos taurus)[J]. Journal of Agricultural Biotechnology, 2018, 26(4):642-651.(in Chinese)
[15] XU X W, ZHENG J, ZOU Q Q, et al.Rapid screening of UPB1 gene variations by high resolution melting curve analysis.[J]. Experimental and Therapeutic Medicine, 2021, 21(4):403-404.
[16] 谭昌渊, 罗九甫, 任兆瑞.高分辨率熔解曲线分析——分子诊断的新技术[J]. 生命科学研究, 2009, 13(3):268-271. TAN C Y, LUO J F, REN Z R.High resolution melting——New method for molecular diagnosis[J]. Life Science Research, 2009, 13(3):268-271.(in Chinese)
[17] 王根生.养牛学[M].北京:中国农业出版社, 2000. WANG G S.Cattle Science[M].Beijing:China Agriculture Press, 2000.(in Chinese)
[18] 王思寒, 田佺, 柳海星, 等.延边黄牛FGF14基因多态性及其对生长性状的影响[J]. 中国畜牧兽医, 2020, 47(12):3993-3999. WANG S H, TIAN Q, LIU H X, et al.Polymorphism of FGF14 gene and its effect on growth traits in Yanbian Yellow cattle[J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(12):3993-3999.(in Chinese)
[19] SHI L J, WU X, YANG Y Z, et al.A post-GWAS confirming the genetic effects and functional polymorphisms of AGPAT3 gene on milk fatty acids in dairy cattle[J]. Journal of Animal Science and Biotechnology, 2021, 12(1):122-140.
[20] SHERMAN E L, NKRUMAH J D, MURDOCH B M, et al.Polymorphisms and haplotypes in the bovine neuropeptide Y, growth hormone receptor, ghrelin, insulin-like growth factor 2, and uncoupling proteins 2 and 3 genes and their associations with measures of growth, performance, feed efficiency, and carcass merit in beef cattle[J]. Journal of Animal Science, 2008, 86(1):1-16.
[21] 艾锦新, 龙安炬, 罗卫星, 等.黔北麻羊UCP2基因组织表达及其多态性与生长性状的关联分析[J]. 中国畜牧兽医, 2020, 47(7):2093-2103. AI J X, LONG A J, LUO W X, et al.Tissue expression of UCP2 gene and its association analysis between polymorphism and growth traits in Qianbei Ma goats[J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(7):2093-2103.(in Chinese)
[22] JIN S H, YANG L, HE T T, et al.Polymorphisms in the uncoupling protein 3 gene and their associations with feed efficiency in chickens[J]. Asian-Australasian Journal of Animal Sciences, 2018, 31(9):1401-1406.
[23] 谢光杰, 王永, 许晴, 等.简州大耳羊肌内脂肪细胞成脂分化差异表达基因的筛选与鉴定[J]. 畜牧兽医学报, 2020, 51(7):1525-1536. XIE G J, WANG Y, XU Q, et al.Selection and validation of the differentially expressed genes during the adipogenic differentiation of Jianzhou Da'er goat intramuscular adipocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7):1525-1536.(in Chinese)
[24] WANG Y N, YANG W C, GUI L S, et al.Association and expression analyses of the UCP2 and UCP3 gene polymorphisms with body measurement and meat quality traits in Qinchuan cattle[J]. Journal of Genetics, 2016, 95(4):939-946.
[25] 国鹏, 封元, 顾亚玲, 等.黑安格斯牛UCP基因多态性位点与生长性状的关联分析[J]. 黑龙江畜牧兽医, 2019, 15:6-9. GUO P, FENG Y, GU Y L, et al.Association analysis of UCP gene polymorphisms and growth traits in Black Angus cattle[J]. Heilongjiang Animal Husbandry and Veterinary Medicine, 2019, 15:6-9.(in Chinese)
[26] 郝军, 柴志欣, 王吉坤, 等.麦洼牦牛UCP基因多态性及其与生长性状的遗传效应分析[J]. 黑龙江畜牧兽医, 2019, 21:149-153. HAO J, CHAI Z X, WANG J K, et al.Analysis of UCP gene polymorphism and its genetic effects associated with growth traits in Maiwa yak[J]. Heilongjiang Animal Husbandry and Veterinary Medicine, 2019, 21:149-153.(in Chinese)

Polymorphism of UCP2 and UCP3 Genes and Its Association with Growth Traits in Yanbian Yellow Cattle

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[2]	LI Mingbo, GAO Qingshan, LI Guanhao, ZHANG Kui, CUI Lianhua, HOU Lina. Effects of Chinese Herbal Medicine Feed Additives on Meat Quality and Flavor Substances in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 531-542.
[3]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[4]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[5]	FENG Xiaopin, LI Yinxia, ZHANG Chenjian, ZHANG Jun, MENG Chunhua, QIAN Yong, CAO Shaoxian. Coning and Polymorphism Identification of PLAG1 Gene and Its Association Analysis with Birth Weight and Body Size Traits in Boer Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3465-3474.
[6]	YANG Tao, ZHANG Mingjie, XU Ran, ZHANG Han, ZHANG Mengmeng, WU Keliang. Study on Genetic Evaluation of Reproductive and Growth Traits in Pigs by BLUP Method [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2971-2981.
[7]	ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Analysis of FGFR2 Gene Polymorphism and Their Correlation with Antler Weight Traits in Sika Deer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3006-3014.
[8]	QIANG Hao, LIANG Peng, MENG Ke, RONG Xuan, FENG Dengzhen. Polymorphism of IGF1R Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1765-1785.
[9]	LI Jingyun, WANG Xinle, ZHANG Yunfei, BAI Junyan, LI Gan, LEI Ying, DONG Zhihao, CHEN Yu, FAN Hongdeng, WANG Longwei, CHEN Mengke, WANG Xingke, SIMA Leiyang, ZHANG Shiwen. Polymorphism of MyoG Gene Intron Ⅱ and Its Association with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1364-1373.
[10]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[11]	DENG Yihua, WANG Tianjiao, WANG Hongliang, DONG Yimeng, LIU Xin, XING Xiumei. Screening of Specific SNP Sites in Tahe Red Deer Based on Resequencing Technology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1413-1421.
[12]	JIANG Hongzheng, YANG Dezhi, MA Zhonghua, XUN Wenjuan, SHI Liguang, HOU Guanyu. Genome-wide Association Study of Body Size Traits in Danzhou Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 598-607.
[13]	WANG Congliang, FU Qi, JU Minying, BAI Jingjing, LI Xinshu, SONG Xiaoyue, LI Longping, LAN Xianyong, QU Lei, ZHANG Lei, ZHU Haijing. The Genetic Polymorphisms of KAP9.2 Gene and Its Correlation Analysis with Growth Traits of Inner Mongolia Cashmere Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4270-4278.
[14]	ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Polymorphism of MSRB3 Gene and Its Association Analysis with Antler Weight Traits in Sika Deer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4279-4287.
[15]	FENG Mian, SHANG Mingyu, BI Yazhen, HU Wenping, ZHANG Li. Polymorphisms of FUT8 Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3838-3845.