N-氨甲酰谷氨酸对奶牛超排效果及血液指标的影响

doi:10.16431/j.cnki.1671-7236.2021.10.021

Abstract

Abstract: The purpose of this study was to explore the effects of N-carbamylglutamate (NCG) feeding for different periods on superovulation and blood biochemical indicators in dairy cows. A total of 16 Holstein heifers were selected and randomly divided into two groups, each with 8 heifers. The control group was fed with a basal diet, and the test group was fed 20 g/(d·head) of NCG on the basis of the basal diet. The effect of feeding period of NCG on the superovulation effect of donor cattle was measured by the FSH method of decreasing injection for 4 consecutive days. Tail root vein blood were collected on the 0, 5, and 9 d of the 3 superovulation treatments to determine hormone indicators and serum biochemical indicators. The results showed that:①NCG was fed for 20 d (the frist superovulation), there were no significant differences in the number of recovered embryos, available embryos, degenerated embryos and unfertilized oocytes between test and control groups (P>0.05). When NCG was fed for 50 d(the second superovulation), the number of embryos recovered in test group was significantly higher than that in control group (P<0.05). When NCG was fed for 80 d (the third superovulation), the number of available embryos in test group was significantly higher than that in control group (P<0.05). In 3 superovulation tests, the number of embryos recovered in the test group was significantly higher than that in control group (P<0.05). ②The feeding period of NCG had no significant effect on the concentrations of FSH, LH, P₄ and E₂ in the blood of donor cattle (P>0.05). It showed that NCG feeding period had no effect or was a secondary influencing factor on the secretion of reproductive hormones, and there was no significant correlation between the change of superovulation effect and the change of reproductive hormones. ③The feeding period of NCG had no significant effect on the concentration of AST in the blood of the donor cattle (P>0.05). The concentration of GLU in the calf serum of the donors in test group was significantly higher than that in control group when NCG was fed for 13 d (P<0.05). The concentration of BUN in the calf serum of the donors in test group was significantly lower than that in control group when NCG was fed for 34 d (P<0.05). The concentration of NO in the calf serum of the donors in test group was significantly higher than that in control group when NCG was fed for 43 and 69 d (P<0.05). In conclusion, each donor heifer was fed with 20 g NCG per day for 3 consecutive superovulation, the number of recovered embryos and the number of available embryos could be increased by 4.98 and 1.8, respectively, and then reduce the production cost of embryos.

Key words: N-carbamylglutamate (NCG); embryo; superovulation; serum hormone level; serum biochemical indicator

CLC Number:

S814.6

LI Shujing, LI Xin, FENG Chuntao, GAO Weijing, SUN Guilai, GU Sukun, YU Wenli. Effect of N-carbamylglutamate on Superovulation and Blood Indexes in Dairy Cows[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3699-3707.

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Effect of N-carbamylglutamate on Superovulation and Blood Indexes in Dairy Cows

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