绒山羊皮肤毛囊兴盛前期组织形态、chi-miR-107-3p与RHBDF2基因表达分析

doi:10.16431/j.cnki.1671-7236.2021.06.004

Abstract

Abstract: The aim of this study was to explore the effect of different expressions of chi-miR-107-3p and rhomboid family member 2(RHBDF2) genes on the villus growth of cashmere goats in different breeds (strains) and light controlled cashmere increasing in the skin hair follicle anagen (May to July).In this experiment,the hair follicle tissues of Inner Mongolia Alashan cashmere goats,Mingai cashmere goats and Albas cashmere goats which were used the increasing cashmere yield technology by shortened photoperiod were randomly collected in July,and HE staining was used to observe the morphology differences.Real-time quantitative PCR was used to detect the ralative expression of chi-miR-107-3p and RHBDF2 genes.The results showed that the secondary hair follicles of Alashan cashmere goats were in the stage of growth and development,and the reconstruction of secondary hair follicles of Mingai cashmere goats and Albas cashmere goats (light controlled cashmere increasing) had been basically completed.The expression of chi-miR-107-3p in the skin tissue of Alashan cashmere goats was extremely significantly higher than that of Mingai cashmere goats and Albas cashmere goats (P<0.01),the expression level of RHBDF2 gene was extremely significantly lower than that of the other two breeds (P<0.01),but the expression level of RHBDF2 gene in skin tissue of Mingai cashmere goats was significantly lower than that of Albas cashmere goats (P<0.05).In conclusion,the microstructure of hair follicle in different breeds (strains) light controled cashmere goats at anagen prosperity was consistent with the results of the differential analysis of chi-miR-107-3p and RHBDF2 genes in skin tissues.The expression level of chi-miR-107-3p was high,while the expression level of RHBDF2 gene was extremely low.With the completion of secondary hair follicle reconstruction,the expression of chi-miR-107-3p decreased significantly,the expression of RHBDF2 gene increased gradually,and the expression mechanism of Inner Mongolia cashmere in different breeds (strains) was basically the same.chi-miR-107-3p and RHBDF2 genes were important regulators for the growth and development of skin hair follicles in cashmere goats.

Key words: Inner Mongolia cashmere goats; light controlled cashmere increasing; histomorphology; chi-miR-107-3p; RHBDF2 gene

CLC Number:

Q786

WANG Lizhong, WU Tiecheng, MA Yuejun, LI Yurong, WU Yahan, HE Tuoya, ZHAO Shengguo, LIU Bin. Expression Analysis of Histomorphology,chi-miR-107-3p and RHBDF 2 Genes of Hair Follicle in Cashmere Goats at Anagen Prosperity[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1918-1926.

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Expression Analysis of Histomorphology,chi-miR-107-3p and RHBDF 2 Genes of Hair Follicle in Cashmere Goats at Anagen Prosperity

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