绵羊B4GALNT2和ESR1基因多态性及其与产羔数的关联分析

doi:10.16431/j.cnki.1671-7236.2021.09.024

Abstract

Abstract: In order to explore the candidate loci affecting the lambing traits in the breeding of high-quality meat sheep in Ningxia, 5 sheep populations of Dorper sheep, Tan×Han hybrid sheep, hybrid generation 1, hybrid generation 2 and cross generation 1 were studied. Flight mass spectrometry technology was used to detect the polymorphism of beta-1, 4-N-acetyl-galactosaminyl transferase 2 (B4GALNT2) gene g. 36933082 G>A and g. 36946470 G>A and estrogen receptor 1 (ESR1) gene g. 75378892 A>T in 5 sheep populations and analyze their association with litter size in sheep. The secondary and tertiary structures of proteins before and after B4GALNT2 and ESR1 genes mutation were analyzed by bioinformatics online software. The results showed that there were three genotypes (AA, AG and GG) in g. 36933082 G>A and g. 36946470 G>A of B4GALNT2 gene, and GG genotype was the dominant genotype. There were three genotypes (AA, TA and TT) in g. 75378892 A>T of ESR1 gene, and AA genotype was the dominant genotype. g. 36933082 G>A and g. 36946470 G>A showed low polymorphism in 5 sheep populations (PIC<0.25), g. 75378892 A>T showed moderate polymorphism in 5 sheep populations (0.25<PIC<0.5), and the three loci were in Hardy-Weinberg equilibrium in 5 sheep populations. The correlation analysis of litter size results showed that g. 36933082 G>A and g. 36946470 G>A of different genotypes were not significantly different from those in 5 sheep populations (P>0.05), but g. 75378892 A>T in hybrid generation 1 sheep population, the litter size of individuals with TA genotype was significantly higher than that of TT genotype (P<0.05). Bioinformatics results showed that all three sites caused changes in the secondary and tertiary structures of the corresponding protein. In conclusion, g. 36933082 G>A and g. 36946470 G>A of B4GALNT2 gene were not suitable for the breeding of multiple lambing traits in 5 sheep populations, while g. 75378892 A>T of ESR1 gene could be considered as a molecular assistant marker for multiple lamb traits in hybrid generation 1 sheep population.

Key words: sheep; B4GALNT2 gene; ESR1 gene; polymorphism; bioinformatics analysis; litter size

CLC Number:

S813.3

RONG Xuan, SHAO Shuncheng, LIANG Peng, ZHANG Tianwen, ZOU Shifan, MENG Ke, QIANG Hao, FENG Dengzhen. Polymorphism of B4GALNT2 and ESR1 Genes and Its Association with Litter Size in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3332-3342.

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Polymorphism of B4GALNT2 and ESR1 Genes and Its Association with Litter Size in Sheep

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