紫锥菊多糖与硫酸化紫锥菊多糖颉颃环磷酰胺对雏鸡免疫抑制作用的研究

doi:10.16431/j.cnki.1671-7236.2019.10.031

Abstract

Abstract: The aim of this study was to investigate the effects of Echinacea purpurea polysaccharide (EPP) and sulfated Echinacea purpurea polysaccharide (SEPP) on immunosuppressive status in chickens.320 14-day-old healthy Lingnan Yellow cocks were randomly divided into blank group (NC),cyclophosphamide group (CY),CY+EPP high,medium and low dose group (EPPH,EPPM and EPPL groups) and CY+SEPP high,medium and low dose group (SEPPH,SEPPM and SEPPL groups).Except NC group,the other 7 groups were injected 80 mg/(kg·d) CY intramuscularly for 3 days.EPPH,EPPM and EPPL groups were administered 10,5,2.5 mg/(kg·d) EPP for 7 consecutive days.SEPPH,SEPPM and SEPL groups were administered 10,5,2.5 mg/(kg·d) SEPP for 7 consecutive days.The body weight,organ indexes of liver,spleen and kidney,transformation of peripheral blood lymphocyte,blood biochemical index,cytokine and antioxidant factor were measured on the 7th,14th,21st and 28th day after the first administration.The results showed that SEPP and EPP could significantly increase the body weight,liver index,spleen index and kidney index of immunosuppressive chickens induced by CY (P<0.05),as well as the transformation of peripheral blood lymphocyte,decrease the concentration of glucose (GLU),urea (UREA) and alkaline phosphatase (ALP),and increase the activity of aspartate aminotransferase (AST).Concentrations of albumin (ALB) and interleukin-2 (IL-2),IL-6 were significantly increased (P<0.05).SEPP was better than EPP.The results showed that oral dose of EPP and SEPP could antagonize the cellular and humoral immunosuppression induced by CY in chickens.SEPP had better effect on lymphocyte transformation in peripheral blood of chickens at different sampling time points than EPP,and SEPP had better effect on IL-2 secretion in peripheral blood of chickens at 21 and 28 days than EPP.

Key words: Echinacea purpurea polysaccharide (EPP); sulfated Echinacea purpurea polysaccharide (SEPP); cyclophosphamide (CY); immunosuppression; transformation of peripheral blood lymphocyte; blood biochemical indicators; cytokines

CLC Number:

R285.5

YAO Lili, BAI Lin, TAN Ying, SUN Jiaqi, SHI Dayou, GUO Shining, LIU Cui. Study on Antagonistic Effects of Echinacea purpurea Polysaccharide and Sulfated Echinacea purpurea Polysaccharide on Immunosuppression of Cyclophosphamide in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3084-3094.

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Study on Antagonistic Effects of Echinacea purpurea Polysaccharide and Sulfated Echinacea purpurea Polysaccharide on Immunosuppression of Cyclophosphamide in Chickens

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