油橄榄叶提取物对酒精性脂肪肝大鼠PP1-DNA-PK-USF1信号通路的干预作用

doi:10.16431/j.cnki.1671-7236.2018.08.035

Abstract

Abstract:

To explore the mechanism of olive leaf extract (OLE) on alcohol hepatic injury,fifty rats were randomly divided into five groups,including blank control group,model group and three different doses groups of OLE.Hepatic injury models of rats were established by using edible alcohol with increasing dosage for 24 weeks.In the process of experimental hepatic injury,the rats in OLE groups were given OLE at doses of 1 000,500 or 250 mg/kg by intragastric administration,respectively.The changes of liver histological structure were observed by bio-microscopy.The levels of serum lipids were determined by automatic biochemistry analyzer.Real-time PCR,Western blotting and ELISA were used to detect mRNA and protein content changes of hepatic protein phosphatase 1 (PP1),DNA-dependent protein kinase (DNA-PK) and upstream stimulating factor 1 (USF1).The results showed that compared with blank control group,hepatic injury in the model group exhibited significant changes,the levels of serum triglyceride (TG) and free fatty acid (FFA) were extremely significantly increased (P<0.01),mRNA levels and protein contents of PP1,DNA-PK and USF1 in liver were extremely significantly increased (P<0.01).Compared with the model group,hepatic pathological changes were meliorated.OLE significantly or extremely significantly reduced the levels of serum TG and FFA (P<0.05;P<0.01),and mRNA levels and protein contents of PP1,DNA-PK and USF1 in liver (P<0.05;P<0.01).The results indicated that OLE could improve relieve fatty degeneration,and prevent the occurrence and development of alcohol hepatic injury by regulation of PP1-DNA-PK-USF1 signaling pathway.

Key words: olive leaf extract; alcoholic fatty liver disease (AFLD); signaling pathway; rat

CLC Number:

R285

WANG Yu, HE Jiujun. Intervention Effect of Olive Leaf Extract on PP1-DNA-PK-USF1 Signaling Pathway of Alcoholic Fatty Liver Disease Rats[J]. , 2018, 45(8): 2327-2333.

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Intervention Effect of Olive Leaf Extract on PP1-DNA-PK-USF1 Signaling Pathway of Alcoholic Fatty Liver Disease Rats

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