酵母细胞壁多糖提取纯化鉴定技术及其应用研究进展

doi:10.16431/j.cnki.1671-7236.2019.12.018

Abstract

Abstract: The main active components of yeast cell wall polysaccharide are β-glucan and mannan,which compete with intestinal receptors to adsorb pathogenic bacteria and to provide energy in intestinal degradation to improve the intestinal environment of animals.They can also be used to chelate with Fe²⁺ ions to inhibit the production of hydroxyl radicals and prevent lipid peroxide chain reaction to improve antioxidant capacity.And its unique chemical sites will adsorb mycotoxins by a variety of intermolecular interactions.β-glucan and mannan enhance the specific and nonspecific immunity of the body by acting on macrophages and regulating a variety of cytokines.Currently,it can be used in aquaculture to replace chlortetracycline,mucin sulfate and other antibiotics to control the necrotizing enteritis of chickens,reduce bacterial infection in aquatic animals,and reduce the diarrhea rate of young animals such as pigs and cattle,prevent all kinds of aging diseases and promote the growth and development of animals.The results of the existing extraction methods of yeast cell wall polysaccharide were compared.The ultrasonic assisted enzyme hydrolysis rate was the highest and the effect was the best when β-glucan was extracted from yeast cell wall.The extraction of mannan by low concentration sodium hydroxide (NaOH) solution and the extraction of yeast cell wall polysaccharide by enzyme-alkali method were the best.The separation and purification techniques include deprotein,decolorization and separation of single polysaccharide.The effect of isolation and purification is compared.The best method to remove the protein from yeast cell wall polysaccharide is Sevage method.The best method of pigment removal is granular activated carbon adsorption,and the method of separation and purification of single polysaccharide by DEAE-cellulose A52 anion exchange column and Sephadex G-100 column is the best.Compared with the existing identification techniques of yeast cell wall polysaccharides,the structure identification of yeast cell wall polysaccharides includes that determination of monosaccharide composition of polysaccharides by high performance liquid chromatography,and uniformity and molecular weight of polysaccharides by high performance gel permeation chromatography.The fine structure of polysaccharide was determined by fourier transformation infrared spectrometer (FT-IR),chromatographic mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR).This review commences with the effects and applications extraction,purification and identification techniques of yeast cell wall polysaccharides.It can provide a theoretical basis for the popularization and application of yeast cell wall polysaccharides as an antibiotic substitute in animal husbandry production and offer a new thinking direction for the research and development of antibiotic substitutes.

Key words: yeast cell wall polysaccharides; function and application; extraction; separation and purification; identification

CLC Number:

S816.7

LIU Yana, ZHOU Zheqi, WANG Fang, WU Xiangyun, PAN Yuanhu, HAO Haihong, CHENG Guyue, WANG Yulian. Research Progress on Application,Extraction,Purification and Identification of Yeast Cell Wall Polysaccharide[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3607-3616.

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Research Progress on Application,Extraction,Purification and Identification of Yeast Cell Wall Polysaccharide

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