DHA对高脂饲粮诱导肝脏脂肪积累的预防作用

doi:10.16431/j.cnki.1671-7236.2022.01.015

Abstract

Abstract: [Objective] This study was to explore the preventive mechanism of docosahexaenoic acid (DHA) on liver fat accumulation induced by high fat diet (HFD). [Method] 32 male mice were divided into 4 groups. Control group (Con) mice were fed with control diet, model group (Model) mice were feed with high fat diet, DHA groups were supplemented with 0.2 g DHA (DHAL) and 1.0 g DHA (DHAH) in high fat diet, respectively. The feeding cycle was 20 weeks. The body weight and diet weight were weighed during feeding, and the food intake was calculated. At the end of feeding, liver and blood were collected, and the contents of liver adiponectin and serum triglyceride (TG) were detected by ELISA. The expression of key enzymes of newborn lipogenesis (SREBP-1c, FAS), key genes of fatty acid oxidation (PPARα, PPARγ, CPT-1A and ACOX), mitochondrial gene (PGC-1α) and brown fatty genes (PRDM16, UCP1) were detected by Real-time quantitative PCR. The expression of the hepatic phosphorylated ACC, AMPK and AKT proteins were determined by the Western blotting. [Result] Compared with Con group, the final body weight, body fat weight and TG content were significantly increased (P<0.05), and hepatic adiponectin level was significantly decreased in Model group (P<0.05). When compared with Model group, the final body weight, body fat weight and TG content of DHAL and DHAH groups were significantly decreased (P<0.05), and liver adiponectin level was significantly increased(P<0.05). The results of Real-time quantitative PCR showed that compared with Con group, SREBP-1c and FAS mRNA level of Model group were significantly increased (P<0.05), PPARα, CPT-1A, ACOX, PGC-1α and UCP1 mRNA level of Model group were significantly decreased (P<0.05). While compared with Model group, SREBP-1c and FAS mRNA levels of both DHAH and DHAL groups were significantly decreased (P<0.05), and the level of DHAH group was lower than that of DHAL group (P<0.05), PPARα, CPT-1A, ACOX, PGC-1α, Prdm16 and UCP1 mRNA expression level of DHAL and DHAH groups were significantly increased (P<0.05), CPT-1A and ACOX mRNA level of DHAH group was higher than that of DAHL group (P<0.05), and there was no significant difference in PPARγ mRNA expression level among the four groups(P>0.05). The results of Western blotting showed that p-ACC in Model group was significantly higher than that in Con and DHAH groups, but significantly lower than that in DHAL group, p-AMPK/AMPK and p-AKT/AKT of Model group were smaller than those of Con group (P<0.05), p-AMPK/AMPK and p-AKT/AKT of DHAH group were bigger than those of Model and DHAL groups (P<0.05). [Conclusion] DHA could reduce the increase of final body weight, body fat and TG content in C57BL/6 mice caused by high fat diet, promote fatty acid oxidation and browning of white adipocytes, so as to prevent fat accumulation in liver.

Key words: high fat food; mice; docosahexaenoic acid (DHA); liver; lipid accumulation

CLC Number:

Q411

AN Xiaoyu, HAO Wei, SUN Liting, YANG Danni, CHANG Yue, ZHAO Ting, LI Kang, LI Yutong, ZHANG Zhijing, YANG Huidi. Preventive Effect of Docosahexaenoic Acid on Liver Lipid Accumulation Induced by High Fat Diet in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 140-149.

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Preventive Effect of Docosahexaenoic Acid on Liver Lipid Accumulation Induced by High Fat Diet in Mice

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