内蒙古地区犊牛腹泻大肠杆菌耐药性分析及毒力基因检测

doi:10.16431/j.cnki.1671-7236.2023.09.037

Abstract

Abstract: 【Objective】 The purpose of the experiment was to understand the drug resistance and virulence gene carrying of Escherichia coli causing calf diarrhea in Inner Mongolia,screen sensitive antibacterial drugs,and guide the rational use of drugs in clinical treatment of calf diarrhea.【Method】 Isolation,purification,biochemical identification and molecular biological identification of Escherichia coli were carried out on the collected calf diarrhea materials.Drug sensitivity test was used to determine the drug resistance phenotype and multiple drug resistance of the isolated strains,and drug resistance genes and virulence genes were detected.【Result】 The isolated strain exhibited a purple black colony with a green metallic luster on Eosin-Methylene blue medium.They reacted positively with indole,methyl red and maltose,but negatively with citrate and H₂S.A 262 bp target band of Escherichia coli specific gene uida was amplified by PCR.A total of 32 strains of Escherichia coli were isolated from 44 diseased materials,with a separation rate of 72.7%.The results of drug sensitivity test showed that Escherichia coli causing calf diarrhea showed multiple drug resistance,and 84.4% of them were resistant to three or more antimicrobial agents,it was significantly resistant to ampicillin,cefotaxime,ciprofloxacin,kanamycin and compound sulfamethoxazole,and relatively sensitive to cefepime,cefoxitin,doxycycline,minocycline and gatifloxacin,especially sensitive to meropenem and polymyxin B.Among the resistance genes β-lactam genes bla_TEM,bla_CTX-M,quinolone resistance genes qnrA,qnrS,tetracycline resistance genes tetA,tetB,tetD and aminoglycoside resistance genes aadA,aadB,aacC,aac(3')Ⅱa were detected,of which aadA and bla_CTX-M genes had the highest detection rates of 100% and 87.5%,respectively.Among the 10 virulence genes tested,only 5 virulence genes Irp2,FyuA, astA,LT-Ⅱ and HlyA were detected,while Sta,Stb,HlyE,Stx1 and Stx2e virulence genes were not detected.【Conclusion】 The phenomenon of multiple drug resistance of Escherichia coli causing calf diarrhea in Inner Mongolia was relatively serious,carrying multiple drug resistance genes such as bla_CTX-Mand aadA,and carrying virulence genes such as Irp2 and FyuA.It was suggested that antibiotics should be selected scientifically and reasonably according to the drug sensitivity test results in clinical practice to effectively prevent and control epidemic diseases.

Key words: calf diarrhea; Escherichia coli; isolation and identification; drug resistance; virulence gene

CLC Number:

S852.61^＋2

SUN Yue, WANG Qi, MAO Wei, WANG Bo, DONG Haiyan, FAN Hongliang, HAO Puguo, ZHAO Hongxia. Drug Resistance Analysis and Virulence Gene Detection of Escherichia coli Causing Calf Diarrhea in Inner Mongolia[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3811-3822.

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Drug Resistance Analysis and Virulence Gene Detection of Escherichia coli Causing Calf Diarrhea in Inner Mongolia

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