藏羊源链球菌的分离鉴定及生物学特性研究

doi:10.16431/j.cnki.1671-7236.2023.09.038

Abstract

Abstract: 【Objective】 In December 2022,unexplained death cases of Tibetan sheep were successively observed in a county of Lhasa,Tibet.This study was aimed to identify the pathogen causing the death of Tibetan sheep in Lhasa,Tibet,and study its biological characteristics,so as to provide scientific basis for the prevention and control of the disease in the future.【Method】 The pathogenic bacteria were isolated and purified from the diseased material in dead Tibetan sheep,and the isolates were identified by morphological characteristics,biochemical properties,16S rRNA PCR amplification and genetic evolutionary analysis.The pathogenicity and drug resistance of the isolates were studied by animal pathogenicity and drug susceptibility tests.【Result】 Eight Gram-positive cocci,named Tibet-1,Tibet-2,Tibet-3,Tibet-4,Tibet-5,Tibet-6,Tibet-7 and Tibet-8,were isolated from the diseased tissues in dead Tibetan sheep.Biochemical identification results showed that all the isolates could ferment maltose,lactose,glucose,sorbitol,aescin,salicylin and decomposed mannitol,and couldn’t decompose urease,starch and xylose.V-P test was negative,and the results conformed to the biochemical characteristics of Streptococcus.The results of 16S rRNA gene sequencing showed that the eight isolates were 95.8%-97.4% similarity to the standard strain of Streptococcus ovis subspecies CCUG 39485T (Y17358).The evolutionary tree showed that the isolates were in the same family as Streptococcus ovis subspecies and in a different branch from other Streptococcus species.Animal pathogenicity tests showed that the isolates were pathogenic to mice of different intensities,with significant lesions in liver,lung and other internal organs of dead mice.Drug susceptibility tests showed that the isolates were sensitive to chloramphenicol,ofloxacin and ciprofloxacin,and were resistant to 13 antimicrobials including cefradin,vancomycin and clindamycin to different degrees,and 7 of the isolates were multi-drug resistant.【Conclusion】 In this study,8 strains of Streptococcus ovis of Tibetan sheep origin were successfully isolated,and the drug resistance of different isolates differed to some extent,providing a reference for clinical medication and prevention and control.

Key words: Tibetan sheep; Streptococcus; isolation and identification; biological characteristics

CLC Number:

S852.61⁺1

WANG Dongjing, SU Zhonghua, YUAN Zhenjie, MA Hongcai, ZENG Jiangyong. Isolation,Identification and Biological Characteristics of Streptococcus Isolated from Tibetan Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3823-3832.

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Isolation,Identification and Biological Characteristics of Streptococcus Isolated from Tibetan Sheep

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