4株广东地区2型猪链球菌耐药性及致病性研究

doi:10.16431/j.cnki.1671-7236.2023.09.030

Abstract

Abstract: 【Objective】 The purpose of this study was to understand the pathogenic serotype,virulence gene characteristics,drug resistance and pathogenicity of suspected Streptococcus suis in pig farms in some areas of Guangdong.【Method】 Bacterial isolation and culture were carried out of samples with suspected Streptococcus suis epidemics in four pig farms,and the isolates were identified by morphological observation,Gram staining microscopy,16S rRNA sequencing,and biochemical testing.Serotype identification and virulence gene detection of the isolated strains were performed by PCR assay.Drug sensitivity tests were conducted on the isolated strains using drug sensitive paper diffusion method,and growth curves were drawn using live bacterial count method.The pathogenicity of each isolate was evaluated by artificial infection of Kunming mice.【Result】 The isolated strain grew small colonies on TSA medium containing 10% adult bovine serum,which were round,gray white,semi transparent,with smooth surfaces and neat edges.Gram positive cocci were detected by microscopy,arranged in a short chain,with biochemical characteristics consistent with Streptococcus suis.After sequencing analysis,it was determined to be Streptococcus suis.The serotype identification results showed that all four isolated strains were Streptococcus suis type 2,with three strains having phenotypes of mrp⁺/epf⁺/orf2⁺/gapdh⁺/sly⁺/fbps⁺/gdh⁺ and one strain having phenotypes of mrp^-/epf^-/orf2^-/gapdh⁺/sly⁺/fbps⁺/gdh⁺,totaling two virulence gene phenotypes.The drug sensitivity results showed that the four isolates were resistant to erythromycin,clindamycin,tetracycline and cotrimoxazole,and sensitive to penicillin and ampicillin.The isolates reached the logarithmic growth phase during 4-6 h,and reached the peak viable bacteria at 6 h of incubation.The mice in the test group showed symptoms such as depression,huddling,shortness of breath and blue tail after infection with Streptococcus suis,followed by death and pathological changes of different degrees on autopsy.The LD₅₀of the four strains were 4.47×10⁷,3.57×10⁷,5.77×10⁷and 1.22×10⁸CFU,respectively.【Conclusion】 This study successfully isolated and identified 4 strains of Streptococcus suis type 2,which had multiple drug resistance and strong pathogenicity.The results of this study could provide theoretical reference for the prevention and control of Streptococcus suis in Guangdong and the screening of vaccine candidate strains.

Key words: Streptococcus suis type 2; isolation and identification; virulence gene phenotype; drug sensitivity test; pathogenicity

CLC Number:

S852.61⁺1

ZI Jiaxin, LIAN Yingjie, CHEN Minyi, LI Qiuxu, ZHANG Weize, LU Mengke, ZHANG Yangyang, QU Haoxuan, MA Jun, BAI Aiquan. Drug Resistance and Pathogenicity of 4 Strains of Streptococcus suis Type 2 in Guangdong[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3740-3751.

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Drug Resistance and Pathogenicity of 4 Strains of Streptococcus suis Type 2 in Guangdong

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