产肠毒素大肠杆菌对断奶仔猪肠道形态、紧密连接蛋白及细胞外基质的影响

doi:10.16431/j.cnki.1671-7236.2023.07.011

Abstract

Abstract: 【Objective】 To explore the effects of enterotoxigenic Escherichia coli (ETEC) on intestinal morphology, tight junction protein and extracellular matrix (ECM) expression of weaned piglets.【Method】 Twelve Duroc weaned piglets aged 35 days were randomly divided into 2 groups (6 replicates per group, 1 pig per replicate):Control group (fed with 20 mL normal saline) and enterotoxin-producing Escherichia coli group (K88 group, fed with 20 mL of 1×10⁹ CFU/mL ETEC K88 suspension), and continuous feeding for 3 days. All piglets were free to eat and drink.On day 1 and day 4, each pig was weighed by repetition and the feed consumption was recorded.The average daily gain (ADG), average daily feed intake (ADFI) and feed to gain ratio (F/G) of each group were calculated.On day 4, the piglets were slaughtered and the intestines were collected, and sliced to observe the intestinal tissue morphology of jejunum and ileum and the content of collagen fiber in the intestines.Real-time quantitative PCR was used to analyze the expression of intestinal compact junction protein gene.Real-time quantitative PCR and western blotting were used to determine the expression of collagen molecules and regulatory enzymes in ECM.【Result】 Compared with control group, the average daily gain of K88 group was significantly decreased (P<0.05), and the average daily feed intake was extremely significantly decreased (P<0.01).The villus height in jejunum and the ratio of villus height to crypt depth in K88 group were extremely significantly decreased (P<0.01), and the villus height in ileum was also significantly decreased (P<0.05).The mRNA expression of Occludin was significantly decreased (P<0.05), and the mRNA expression of Claudin-1 was extremely significantly decreased (P<0.01) in jejunum.At the same time, the mRNA expressions of Occludin and ZO-1 were extremely significantly decreased (P<0.01), and the mRNA expression of Claudin-1 was also significantly decreased (P<0.05) in ileum.The content of collagen fiber in both jejunum and ileum was significantly reduced (P<0.05).The mRNA expressions of type Ⅳ collagen α2 chain (COL4A2), COL5A1 and COL6A1 in jejunum were extremely significantly decreased (P<0.01), and the protein expression of COL4A2 and COL6A1 in the jejunum of K88 group were significantly decreased (P<0.05).The relative mRNA expression of MMP9 in jejunum was significantly increased (P<0.05), while the relative mRNA expression of PAs was highly significantly decreased (P<0.01), and the MMP9 protein content was extremely significantly increased in K88 group (P<0.01).【Conclusion】 In conclusion, feeding ETEC K88 reduced average daily gain and average daily feed intake, damaged intestinal morphology and reduced the expression of intestinal tight junction protein in weaned piglets.In addition, ECM changes could be induced by reducing the content of collagen fibers and the expression of collagen molecules and ECM regulatory enzyme system in the intestine.

Key words: enterotoxigenic Escherichia coli; weaned piglets; intestinal health; extracellular matrix

CLC Number:

S852.61

LIU Chunyan, TANG Qingsong, YANG Bijing, WU Qiwen, XIONG Yunxia, HUANG Yanna, YANG Xuefen, WANG Li, JIANG Zongyong, YI Hongbo. Effects of Enterotoxigenic Escherichia coli on Intestinal Morphology, Tight Junction Protein and Extracellular Matrix of Weaned Piglets[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2697-2706.

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Effects of Enterotoxigenic Escherichia coli on Intestinal Morphology, Tight Junction Protein and Extracellular Matrix of Weaned Piglets

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