基于转录组测序挖掘犊牦牛肾周脂质沉积关键基因

doi:10.16431/j.cnki.1671-7236.2024.07.001

Abstract

Abstract: 【Objective】 This study was aimed to compare the changes in transcriptome expression profiles of kidney-adjacent adipose tissue in yak calves at different ages,and explore the signaling pathways and key genes that regulated kidney-adjacent lipid deposition in yak calves.【Method】 Three healthy male yak calves at 1,7 and 30 day-old were bled to death,and the kidney-adjacent adipose tissue were collected for transcriptome sequencing using Illumina HiSeq platform,after which the transcriptome data were subjected to quality control,comparison,differentially expressed gene (DEGs) screening,and the DEGs were performed to GO function and KEGG pathway enrichment analysis.In addition,seven genes were randomly selected for Real-time quantitative PCR verification.【Result】 Transcriptome results showed that a total of 1 627 significantly DEGs were identified in the comparison group of 1- and 7-day-old,of which 939 were up-regulated and 688 were down-regulated.Also,a total of 3 979 significantly DEGs were identified in the comparison group of 7- and 30-day-old,of which 1 925 were up-regulated and 2 054 were down-regulated.GO function and KEGG pathway enrichment analysis showed that the DEGs were enriched in acyl-CoA dehydrogenase activity to participate in fatty acid biosynthesis,insulin resistance,PPAR signaling pathway, sphingolipid signaling pathway, etc. in the comparison group of 1- and 7-day-old.In addition,the DEGs were enriched in several biological processes,including fatty acid metabolism,lipid metabolism,phosphohydrolase activity and lipase activity to participate in butyrate metabolism,fatty acid metabolism,glycerophospholipid metabolism,phospholipase D signaling pathway,AMPK signaling pathway,etc. in the comparison group of 7- and 30-day-old.The enriched pathways showed that SCD,ADIPOQ,PLIN1,ACADM and LPL were identified as candidate genes associated with kidney-adjacent lipid deposition in yak calves.Real-time quantitative PCR results showed that the expression of seven genes were consistent with RNA-Seq results.【Conclusion】 Five genes related to adipocyte differentiation and fat deposition were screened in this study,including SCD,ADIPOQ,PLIN1,ACADM and LPL.The results provided a theoretical reference for further research on kidney-adjacent lipid deposition in yak calves at different growth stages.

Key words: yak calves; kidney-adjacent adipose tissue; transcriptome; lipid deposition

CLC Number:

ZHAO Yutian, CHEN Fuju, YI Pingchang, MA Min, WANG Shouning, QUAN Qishiliu. Exploring Candidate Genes Related to Kidney-adjacent Lipid Deposition in Yak Calves Based on Transcriptome Sequencing[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2727-2738.

References

[1] CANNON B,NEDERGAARD J.Brown adipose tissue:Function and physiological significance[J].Physiological Reviews,2004,84(1):277-359.
[2] COLLIER R J,GEBREMEDHIN K G.Thermal biology of domestic animals[J].Annual Review of Animal Biosciences,2015,3(1):513-532.
[3] CARSTENS G E,MOSTYN P M,LAMMOGLIA M A,et al.Genotypic effects on norepinephrine-induced changes in thermogenesis,metabolic hormones,and metabolites in newborn calves[J]. Journal of Animal Science,1997,75(7):1746-1755.
[4] CASTEILLA L,CHAMPIGNY O,BOUILLAUD F,et al.Sequential changes in the expression of mitochondrial protein mRNA during the development of brown adipose tissue in bovine and ovine species.Sudden occurrence of uncoupling protein mRNA during embryogenesis and its disappearance after birth[J].Biochemical Journal,1989,257(3):665-671.
[5] ADELA U,ISMAEL G G,ÁNXELA S E,et al.BMP8 and activated brown adipose tissue in human newborns[J].Nature Communications,2021,12(1):5274.
[6] SMITH S B,CARSTENS G E,RANDEL R D,et al.Brown adipose tissue development and metabolism in ruminants[J].Journal of Animal Science,2004,82(3):942-954.
[7] VERMOREL M,DARDILLAT C,DEMIGNE C.Energy metabolism and thermoregulation in the newborn calf[J].Annales of Recherches Veterinaires,1983,14(4):382-389.
[8] KENJI I,TETSUYA Y.UCP1 dependent and independent thermogenesis in brown and beige adipocytes[J].Frontiers in Endocrinology,2020,11:498.
[9] MCNAMARA J P,HUBER K.Metabolic and endocrine role of adipose tissue during lactation[J].Annual Review Animal Biosciences,2018,6(1):177-195.
[10] 李唐,郑立,贺花,等.影响棕色脂肪代谢的调控因素及其应用[J].家畜生态学报,2017,38(12):6-10. LI T,ZHENG L,HE H,et al.Regulation factors affecting brown adipose tissue metabolism and their applications[J].Journal of Domestic Animal Ecology,2017,38(12):6-10.(in Chinese)
[11] MONTANARI T,POŠĆIĆ N,COLITTI M.Factors involved in white-to-brown adipose tissue conversion and in thermogenesis:A review[J].Obesity Reviews,2017,18(5):495-513.
[12] KOTZBECK P,GIORDANO A,MONDINI E,et al.Brown adipose tissue whitening leads to brown adipocyte death and adipose tissue inflammation[J].Journal of Lipid Research,2018,59(5):784-794.
[13] WU J,BOSTRÖM P,SPARKS L M,et al.Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human[J].Cell,2012,150(2):366-376.
[14] KLEINI A H,REVICZKY A,CHOU P,et al.Development of brown adipose tissue thermogenesis in the ovine fetus and newborn[J]. Endocrinology,1983,112:1662-1666.
[15] LIU X,ZHU Y,ZHAN S,et al.RNA-Seq reveals miRNA role in thermogenic regulation in brown adipose tissues of goats[J].BMC Genomics,2022,23(1):186.
[16] TRAYHURN P,THOMAS M E,KEITH J S,et al.Postnatal development of uncoupling protein,uncoupling protein mRNA,and GLUT4 in adipose tissues of goats[J]. American Journal of Physiology,1993,265(3 Pt2):R676-R682.
[17] WANG L,YANG X,ZHU Y,et al.Genome-wide identification and characterization of long noncoding RNAs of brown to white adipose tissue transformation in goats[J]. Cells,2019,8(8):904.
[18] WANG Y,CHEN X,FAN W,et al.Integrated application of metabolomics and RNA-Seq reveals thermogenic regulation in goat brown adipose tissues[J]. FASEB Journal,2021,35(9):e21868.
[19] BASSE A L,DIXEN K,YADAV R,et al.Global gene expression profiling of brown to white adipose tissue transformation in sheep reveals novel transcriptional components linked to adipose remodeling[J].BMC Genomics,2015,16(1):215.
[20] QUESADA-LÓPEZ T,GAVALDÀ-NAVARRO A,MORÓN-ROS SAMANTHA,et al.GPR120 controls neonatal brown adipose tissue thermogenic induction[J].American Journal of Physiology.Endocrinology and Metabolism,2019,317(5):E742-E750.
[21] 陈付菊,赵宇田,马敏.犊牦牛肾周棕色脂肪组织的发育及其产热相关基因的表达分析[J].中国兽医杂志,2024,60(1):44-51. CHEN F J,ZHAO Y T,MA M.Analysis of the development of brown adipose tissue and expression of thermogenic-related genes in perirenal fat of yak calves[J].Chinese Journal of Veterinary Medicine,2024,60(1):44-51.(in Chinese)
[22] SUN Z,GONG J,WU H,et al.Perilipin1 promotes unilocular lipid droplet formation through the activation of Fsp27 in adipocytes[J].Nature Communications,2013,4(1):855-860.
[23] SZTALRYD C,BRASAEMLE D.The perilipin family of lipid droplet proteins:Gatekeepers of intracellular lipolysis[J].Biochim Biophys Acta,2017,1862:1221-1232.
[24] LI B,WENG Q,DONG C,et al.A key gene,PLIN1,can affect porcine intramuscular fat content based on transcriptome analysis[J].Genes,2018,9(4):194-210.
[25] 雷召雄,户春丽,潘翠丽,等.Perilipin在牛脂肪细胞分化过程中的表达模式[J].中国畜牧杂志,2022,58(2):136-143. LEI Z X,HU C L,PAN C L,et al.The expression pattern of Perilipin in the differentiation process of bovine adipocytes[J].Chinese Journal of Animal Science,2022,58(2):136-143.(in Chinese)
[26] SUN Y,ZHAI G,LI R,et al.RXRα positively regulates expression of the chicken PLIN1 gene in a PPARγ-independent manner and promotes adipogenesis[J].Frontiers in Cell and Developmental Biology,2020,14(8):349-361.
[27] 张少华,赵志武,王君实,等.沉默PLIN1基因与异丙肾上腺素对3T3-L1脂肪细胞脂解的机制探究[J].现代预防医学,2018,45(11):2023-2027. ZHANG S H,ZHAO Z W,WANG J S,et al.Combined effect of PLIN1 gene silencing and isoproterenol on lipolysis of 3T3-L1 adipocytes[J]. Modern Preventive Medicine,2018,45(11):2023-2027.(in Chinese)
[28] KIM J J,MIURA R.Acyl-CoA dehydrogenases and acyl-CoA oxidases.Structural basis for mechanistic similarities and differences[J].European Journal of Biochemistry,2004,271(3):483-493.
[29] 唐可人,李盼,段梦琪,等.藏猪和大约克猪ACADM基因表达量与脂肪沉积性状的相关性分析[J].畜牧与兽医,2021,53(2):8-12. TANG K R,LI P,DUAN M Q,et al.Correlation analysis between the ACADM gene expression and fat depositon traits in Tibetan and Yorkshire pigs[J]. Animal Husbandry&Veterinary Medicine,2021,53(2):8-12.(in Chinese)
[30] GRABNER G F,XIE H,SCHWEIGER M,et al.Lipolysis:Cellular mechanisms for lipid mobilization from fat stores[J].Nature Metabolism,2021,3(11):1445-1465.
[31] 蔡刚志,华再东,毕延震,等.SCD基因与猪脂肪酸代谢(综述)[J].江苏农业科学,2019,47(20):24-28. CAI G Z,HUA Z D,BI Y Z,et al.SCD gene and fatty acid metabolism in pigs:A review[J].Jiangsu Agricultural Sciences,2019,47(20):24-28.(in Chinese)
[32] XIN Y,SHENQIANG H,LIANG L,et al.Metabolomic analysis of SCD during goose follicular development:Implications for lipid metabolism[J]. Genes,2020,11(9):1001.
[33] ALJOHANI A M,SYED D N,NTAMBI J M.Insights into stearoyl-CoA desaturase-1 regulation of systemic metabolism[J].Trends in Endocrinology Metabolism,2017,28(12):831-842.
[34] 张海波,官久强,周建旭,等.牦牛不同肌肉组织的肌内脂肪含量与脂肪代谢相关基因表达的相关性分析[J].中国畜牧杂志,2020,56(8):73-77. ZHANG H B,GUAN J Q,ZHOU J X,et at.Correlation analysis of intramuscular fat content and fat metabolism related gene expression in different muscle tissues of yaks[J].Chinese Journal of Animal Science,2020,56(8):73-77.(in Chinese)
[35] 刘自增,阎萍.天祝白牦牛Leptin和SCD1基因单核苷酸多态性与肌肉脂肪酸含量的关联性分析[J].中国畜牧兽医,2011,38(4):154-159. LIU Z Z,YAN P.Association analyses of SNPs in the Lpetin and SCD1 gene on the fatty acid content of intramuscular fat in Tianzhu White yak[J].China Animal Husbandry&Veterinary Medicine,2011,38(4):154-159.(in Chinese)
[36] 罗宗刚,李祥,李明洲,等.脂联素(AdipoQ)及其受体基因(AdipoR1和AdipoR2)在猪背最长肌和腰大肌中的表达差异[J].农业生物技术学报,2011,19(3):507-512. LUO Z G,LI X,LI M Z,et al.Expression changes of adiponection (AdipoQ) and its receptor genes (AdipoR1 and AdipoR2) in porcine longissimus dorsi and psoas major musscles[J].Journal of Agricultural Biotechnology,2011,19(3):507-512.(in Chinese)
[37] 唐妮,王书瑶,齐锦雯,等.脂联素调控脂质代谢的研究进展[J].畜牧兽医学报,2018,49(12):2550-2557. TANG N,WANG S Y,QI J W,et al.Research progress on adiponectin regulating lipid metabolism[J].Acta Veterinaria et Zootechnica Sinica,2018,49(12):2550-2557.(in Chinese)
[38] FU Y,LUO N,KLEIN R L,et al.Adiponectin promotes adipocyte differentiation,insulin sensitivity,and lipid accumulation[J].Journal of Lipid Research,2005,46(7):1369-1379.
[39] 张辉,李鹏,闫峰,等.日粮能量水平对延边黄牛肌内脂肪含量与脂联素表达水平的影响[J].中国饲料,2010,10:21-23. ZHANG H,LI P,YAN F,et al.Effects of dietary energy level on intramuscular fat content and expression level of ADPN in Yanbian cattle[J]. China Feed,2010,10:21-23.(in Chinese)
[40] 张萌萌,魏胜娟,王艺如,等.牛肌内前体脂肪细胞的分离培养及分化相关基因的表达规律研究[J].畜牧与兽医,2018,50(5):1-6. ZHANG M M,WEI S J,WANG Y R,et al.Primary culture and expression of differentiation-related genes in bovine intramuscular preadipocytes[J].Animal Husbandry&Veterinary Medicine,2018,50(5):1-6.(in Chinese)
[41] 单体中,汪以真,李民.猪脂蛋白脂酶基因片段的克隆及不同体重的表达差异[J].农业生物技术学报,2006,9(2):151-155. SHAN T Z,WANG Y Z,LI M.Cloning of lipoprotein lipase (LPL) gene of swine and the difference of LPL gene expression at different avoirdupois stages[J].Journal of Agricultural Biotechnology,2006,9(2):151-155.(in Chinese)
[42] TAN M H.The lipoprotein lipase system:New understandings[J]. Canadian Medical Association Journal,1978,118(6):675-680.
[43] 高思,徐亚欧,毛亮,等.藏绵羊脂蛋白脂酶基因克隆及序列分析[J].安徽农业科学,2011,39(12):7291-7294. GAO S,XU Y O,MAO L,et al.Tibetan sheep LPL gene clone and bioinformatic analysis[J].Journal of Anhui Agricultural Sciences,2011,39(12):7291-7294.(in Chinese)
[44] 黄艳娜,李莉,何少婧,等.陆川猪脂蛋白脂酶基因的克隆与序列分析[J].南方农业学报,2014,45(6):1074-1078. HUANG Y N,LI L,HE S J,et al.Gene cloning and sequence analysis of lipoprotein lipase in Luchuan pig[J].Journal of Southern Agriculture,2014,45(6):1074-1078.(in Chinese)
[45] 王晶,彭广南,蒋钦杨,等.广西三黄鸡脂蛋白脂酶基因的克隆及蛋白构象分析[J].南方农业学报,2013,44(3):501-506. WANG J,PENG G N,JIANG Q Y,et al.Gene cloning and protein confirmation analysis of lipoprotein lipase in Guangxi Sanhuang chicken[J].Journal of Southern Agriculture,2013,44(3):501-506.(in Chinese)
[46] 李柱,谭支良,韩雪峰,等.脂肪酸转运蛋白家族(FATPs)的研究进展[J].生命科学研究,2013,17(4):348-353. LI Z,TAN Z L,HAN X F,et al.Progress on fatty acid transport proteins (FATPs)[J].Life Science Research,2013,17(4):348-353.(in Chinese)
[47] LUIZ H P,RAFAEL T S,DEBORA E F,et al.Explaining meat quality of bulls and steers by differential proteome and phosphoproteome analysis of skeletal muscle[J].Journal of Proteomics,2019,199:51-66.

Exploring Candidate Genes Related to Kidney-adjacent Lipid Deposition in Yak Calves Based on Transcriptome Sequencing

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Shunbo, WANG Hao, LIANG Yunyi, WANG Wenjing, LI Zicong, XU Zheng. Effects of Feeding Risperidone During Late Pregnancy on Mammary Gland Development of Maternal Mice and Growth of Offspring Mice [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2489-2500.
[2]	LIU Lili, BIAN Yuan, WU Shenglong, BAO Wenbin, WU Zhengchang. Effect and Mechanism of Interferon Stimulated Gene ISG15 on Porcine Epidemic Diarrhea Virus Replication [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2545-2555.
[3]	LU Qingwei, TANG Sen, LI Bin, QIN Chongkai, LIU Wenna, PU Xue, WANG Yaqian, WU Cuiling, FU Xuefeng. Screening of circRNA Related to Secondary Hair Follicle Cycle Development in Jiangnan Cashmere Goats Based on Transcriptome Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 1793-1806.
[4]	TIAN Zuoning, XING Kai, ZHANG Yinhua, BAO Shuai, CAO Yongchun. Identification of Differentially Expressed Genes Affecting Meat Quality Traits in Pigs Using Integrated Transcriptome Sequencing Data [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 1947-1957.
[5]	JIANG Liren, FENG Helong, JIANG Xinrui, WANG Zichen, ZHANG Gaofeng, SHANG Yu, ZENG Zhe, YAO Lun, WANG Hongcai, LUO Qingping, TIAN Guangming, WEN Guoyuan. Transcriptomic Analysis of Host’s Bursa of Fabricius After in-ovo Vaccination with Newcastle Disease Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1632-1641.
[6]	SUN Xiangli, SHI Ziyao, LI Keke, LIU Yuxuan, WANG Ruolin, LIU Yang, WANG Yanbin. Effect of Feeding Clostridium butyricum on the Morphology and Transcriptomics of Cecum Mucosa in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3584-3593.
[7]	LIU Ling, WANG Shengnan, WANG Dandan, MA Yuehui, JIANG Lin, CUI Kai. Effect and Molecular Mechanism of Zbed6 Gene Knockout on the Growth and Development of Skeletal Muscle in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3641-3651.
[8]	GUO Panpan, LI Qiang, LI Xiangzi, YAN Changguo, JIN Xin. Effect of Overexpression of DGAT2 Gene on Differentiation of Yanbian Bovine Preadipocytes [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3045-3055.
[9]	NI Ligang, ZHOU Chunbao, XU Pan, ZHANG Yaqin, TAO Yong, ZHANG Junsheng. Analysis of Differentially Expressed Genes and Regulation Pathway of Backfat Tissue in Sujiang Pigs at Different Growth Stages [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2595-2605.
[10]	YU Ziyu, ZHANG Bingqi, CAI Yuanrong, HONG Longsheng, LI Wanyan, CAO Nan, HUANG Yunmao, XU Danning, TIAN Yunbo, CHEN Wenbin, LI Bingxin. Screening of Genes Related to Bursa of Fabricius Injury Induced by LPS in Magang Geese [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2639-2650.
[11]	YAN Hang, HAO Wen, WANG Chengqian, WANG Huie. Differentially Expressed Genes and Bioinformatics Analysis of Superovulation Ovary in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2740-2754.
[12]	CHEN Kaiwen, ZHAO Yunjiao, WANG Lei, ZHANG Jianbo, MA Yuhong, WU Guofang. Transcriptome Analysis of Porcine Leydig Cells Under Different Oxygen Concentrations [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2185-2195.
[13]	SHI Jingu, HUANG Shanshan, HUANG Jiwei, CHEN Kunhai, HU Dasheng, ZHU Zhixiong, HAN Shuyu. Comparative Transcriptome Analysis of Streptococcus agalactiae from Tilapia Under High Temperature Stress [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2207-2216.
[14]	DU Jiawen, REN Wenyi, KANG Yan, XU Xiaofeng, ZHANG Lili. Transcriptome Comparison and Fatty Acid Analysis of Muscle Tissue in Tan Sheep at Different Ages [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 4925-4934.
[15]	WEI Rong, DENG Zhanzhao, CAO Guowei, ZHU Hongfang, YU Baojun, ZHAO Wei, CHEN Yafei, ZHANG Tong, ZHANG Juan. Genome-wide Association Study and Transcriptomics Combined to Analyze the Regulatory Mechanisms of Eggshell Color in Jingyuan Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4403-4413.