绵羊超数排卵卵巢差异表达基因及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2023.07.015

Abstract

Abstract: 【Objective】 The purpose of this study was to screen out the key genes, biological processes and signaling pathways affecting the development of ovarian follicles in Duolang sheep treated with superovulation, so as to provide reference for further understand the mechanism of ovarian growth and development in Duolang sheep under superovulation.【Method】 After superovulation treatment of 15 Duolang sheep aged 2-3 years old and in good physical condition, the ovarian tissues at the early (11 d), middle (13 d) and late (15 d) stages of superovulation were taken out for transcriptome sequencing by high-throughput sequencing technology.The reads sequence obtained by sequencing was aligned with the reference genome to screen out the differentially expressed genes.The protein interaction network of the differentially expressed genes was analyzed by STRING database, and the Cytoscape software was used for visual editing.GO function and KEGG pathway enrichment analysis were performed using eggNOG-mapper software.【Result】 A total of 223 differentially expressed genes were screened between days 11 and 13 of supernumerary ovulation ovaries, and 259 interactions were obtained.The differentially expressed genes were significantly annotated in entries such as cell growth, ribonucleoprotein complex, etc.Among them, the genes with significant differential expression included ITGB3 and SNAI1, but no significant enrichment pathways were found.A total of 694 differentially expressed genes were screened between day 13 and 15 of supernumerary ovulation ovaries, and 785 interactions were obtained.The differentially expressed genes were significantly annotated in initiation of DNA replication, DNA replication and DNA metabolic processes, etc.The genes with significant differential expression mainly included CASP3, NOTCH1, WNT2 and RUNX2.There were 2 enriched pathways of DNA replication and cell cycle signalling pathway.A total of 238 differentially expressed genes were screened between days 11 and 15 of supernumerary ovulation ovaries, and 151 interactions were obtained.The differentially expressed genes were significantly annotated in the entries of protein hydrolysis, intracellular signaling, etc.The genes with significant differential expression mainly included PRKAR2B, AQP5 and HEY2.There were 2 significantly enriched pathways of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) signaling pathways.GO function and KEGG pathway results were used to screen out 10 genes with direct or indirect functions in the development of ovarian follicles in sheep:ITGB3, SNAI1, CASP3, PRKAR2B, AQP5, NOTCH1, RUNX2, WNT2, DPP10 and HEY2.【Conclusion】 In this study, 10 differentially expressed genes were screened from different developmental stages of superovulation ovary, and found that their related functional roles were consistent with the mechanism of ovarian follicular development in different periods, indicating that these genes might be the key genes that directly or indirectly affect ovarian follicular development, in order to provide theoretical reference for exploring the mechanism of ovarian follicular development in superovulation.

Key words: sheep; superovulation; transcriptome sequencing; differentially expressed genes; ovarian follicles

CLC Number:

YAN Hang, HAO Wen, WANG Chengqian, WANG Huie. Differentially Expressed Genes and Bioinformatics Analysis of Superovulation Ovary in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2740-2754.

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Differentially Expressed Genes and Bioinformatics Analysis of Superovulation Ovary in Sheep

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