绵羊MSTN基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.07.017

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the polymorphism of myostatin (MSTN) gene and its effect on meat quality traits in sheep, in order to provide effective genetic molecular marker for the selection and breeding of meat performance in sheep.【Method】 The variation sites of MSTN gene in 91 sheep including Dupo sheep (D), Tan sheep (T) and Small-tailed Han sheep (XH) were obtained using liquid phase capture sequencing technology, and the single nucleotide polymorphism (SNP) with significant interspecific differences were screened.A total of 30 sheep (D, T, XH and Dupo×Tan×Small-tailed Han sheep (DTH)) were genotyped for the selected site using in-flight mass spectrometry to analyze its association with meat quality traits in sheep.【Result】 Three genotypes were detected for rs129059715 of MSTN gene in 4 sheep populations, including 15 CC genotypes, 9 CA genotypes and 6 AA genotypes, with C being the dominant allele (except for Dupo×Tan×Small-tailed Han sheep). rs129059715 was in Hardy-Weinberg equilibrium in Dupo, Tan and Dupo×Tan×Small-tailed Han sheep populations (P>0.05), and was in Hardy-Weinberg disequilibrium in Small-tailed Han sheep population (P<0.05).rs129059715 was low polymorphic in Dupo and Small-tailed Han sheep populations (PIC<0.25), and was moderately polymorphic in Tan and Dupo×Tan×Small-tailed Han sheep populations (0.25<PIC<0.50).Association analysis revealed that the backfat thickness and trans-linoleic acid content of CA genotype at rs129059715 were extremely significantly higher than that of CC genotype (P<0.01), the net meat percentage, kidney weight, the contents of fat, undecanoic acid, decanoic acid and decanoic acid of CA genotype were significantly higher than that of CC genotype (P<0.05).The muscle fibre diameter of CC genotype was significantly higher than that of AA genotype (P<0.05), pH of CC genotype was significantly higher than that of CA genotype (P<0.05).There were no significant differences with other traits among different genotypes (P>0.05).【Conclusion】 Through liquid phase capture sequencing technology, rs129059715 of MSTN gene with significant differences was obtained, which had significant effects on the slaughter performance, meat quality and fatty acid content of sheep.rs129059715 could be used as a potential candidate marker for meat production performance and meat quality.

Key words: sheep; MSTN gene; polymorphism; slaughter performance; meat quality

CLC Number:

S813.3

TAO Maohai, MENG Ke, RONG Xuan, QIANG Hao, NIE Wei, GUO Chenhao, FENG Dengzhen. Polymorphism of MSTN Gene and Its Association Analysis with Meat Quality Traits in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2766-2776.

References

[1] BI Y, FENG B, WANG Z, et al.Myostatin (MSTN) gene indel variation and its associations with body traits in Shaanbei White cashmere goat[J].Animals, 2020, 10(1):168.
[2] LI W, LI R, WEI Y, et al.Effect of MSTN mutation on growth and carcass performance in Duroc×Meishan hybrid population[J].Animals, 2020, 10(6):932.
[3] SMOłUCHA G, KOZUBSKA-SOBOCINSKA A, KOSENIUK A, et al.Polymorphism of the myostatin (MSTN) gene in Landes and Kielecka geese breeds[J].Animals, 2019, 10(1):10.
[4] KHASANAH H, GUNAWAN A, PRIYANTO R, et al.Polymorphism of myostatin (MSTN) promoter gene and its association with growth and muscling traits in Bali cattle[J].Media Peternakan, 2016, 39(2):95-103.
[5] WANG J, ZHOU H, HU J, et al.Two single nucleotide polymorphisms in the promoter of the ovine myostatin gene (MSTN) and their effect on growth and carcass muscle traits in New Zealand Romney sheep[J].Journal of Animal Breeding and Genetics, 2016, 133(3):219-226.
[6] 郭亚男, 杨茹艳, 张芷毓, 等.MSTN基因干扰通过上调基因Cpt1b促进肌间脂肪的β氧化[J].生物工程学报, 2022, 38(8):3076-3089. GUO Y N, YANG R Y, ZHANG Z Y, et al.MSTN knockdown promotes intramuscular fatty acid metabolism by β oxidation via the up-regulation of Cpt1b[J].Journal of Biological Engineering, 2022, 38(8):3076-3089.(in Chinese)
[7] STERNSTEIN I, REISSMANN M, MAJ D, et al.A new single nucleotide polymorphism in the rabbit (Oryctolagus cuniculus) myostatin (MSTN) gene is associated with carcass composition traits[J].Animal Genetics, 2014, 45(4):596-599.
[8] GAN S Q, DU Z, LIU S R, et al.Association of SNP haplotypes at the myostatin gene with muscular hypertrophy in sheep[J].Asian Australasian Journal of Animal Sciences, 2008, 21(7):928-935.
[9] KIJAS J W, MCCULLOCH R, EDWARDS J E H, et al.Evidence for multiple alleles effecting muscling and fatness at the ovine GDF8 locus[J].BMC Genetics, 2007, 8(1):80.
[10] CRISPO M, MULET A P, TESSON L, et al.Efficient generation of myostatin knock-out sheep using CRISPR/Cas9 technology and microinjection into zygotes[J].PLoS One, 2015, 10(8):e0136690.
[11] GROCHOWSKA E, BORYS B, MROCZKOWSKI S.Effects of intronic SNPs in the myostatin gene on growth and carcass traits in colored Polish Merino sheep[J].Genes (Basel), 2019, 11(1):2.
[12] 张军霞, 雷秀存, 贺娜, 等.藏羊MSTN基因表达及其与产肉性状的关联性分析[J].农业生物技术学报, 2022, 30(5):926-934. ZHNG J X, LEI X C, HE N, et al.Expression of MSTN gene and its association analysis with meat quality in Tibetan sheep (Ovis aries)[J].Journal of Agricultural Biotechnology, 2022, 30(5):926-934.(in Chinese)
[13] DU W, ZHANG Y, YANG J Z, et al.Effect of MSTN propeptide protein on the growth and development of Altay lamb muscle[J].Genetics and Molecular Research, 2016, 15(2):15027778.
[14] 朱菲菲, 张俊星, 张林林, 等.干扰MSTN对牛骨骼肌卫星细胞增殖分化的影响[J].中国畜牧兽医, 2020, 47(2):479-487. ZHU F F, ZHANG J X, ZHANG L L, et al.Effect of interfering with MSTN on the proliferation and differentiation of bovine skeletal muscle satellite cells[J]. China Animal Husbandry & Veterinary Medicine, 2020, 47(2):479-487.(in Chinese)
[15] 赵正伟, 赵涛, 殷骥, 等.中草药添加剂对滩羊生长、免疫性能及肉品质的影响[J].中国畜牧杂志, 2020, 56(11):150-153. ZHAO Z W, ZHAO T, YIN J, et al.Effects of Chinese herbal medicine additives on growth, immune performance, and meat quality of Tan sheep[J].Chinese Journal of Animal Science, 2020, 56(11):150-153.(in Chinese)
[16] 杨杜录, 杨东贵, 安玉峰, 等.青海高原型藏羊与澳洲白羊、黑头杜泊羊、黑头萨福克羊杂交效果的研究[J].中国草食动物科学, 2019, 39(6):70-72. YANG D L, YANG D G, AN Y F, et al.Study on the crossbreeding effect of Qinghai plateau-type Tibetan sheep with Australian White sheep, Black-headed Dupo sheep and Black-headed Suffolk sheep[J].Chinese Herbivore Science, 2019, 39(6):70-72.(in Chinese)
[17] 牛春波.澳洲白羊与小尾寒羊杂交F1产肉性能测定[J].中国畜禽种业, 2020, 16(8):91. NIU C B.Determination of meat production performance of Australian White sheep crossed with Small-tailed Han sheep in F1[J]. China Livestock and Poultry Breeding Industry, 2020, 16(8):91.(in Chinese)
[18] 孟科, 荣轩, 梁鹏, 等.绵羊BMP2、BMP4和BMP7基因多态性与产羔数的关联分析[J].中国畜牧杂志, 2022, 58(5):113-118. MENG K, RONG X, LIANG P, et al.Polymorphism of sheep BMP2, BMP4 and BMP7 genes and its association with litter size[J].Chinese Journal of Animal Science, 2022, 58(5):113-118.(in Chinese)
[19] 孟科, 张天闻, 梁鹏, 等.绵羊MYF5基因多态性及其与生长性状的关联分析[J].农业生物技术学报, 2022, 30(3):496-505. MENG K, ZHANG T W, LIANG P, et al.Polymorphism of MYF5 gene and its association analysis with growth traits in sheep (Ovis aries)[J].Journal of Agricultural Biotechnology, 2022, 30(3):496-505.(in Chinese)
[20] 曹红鹤, 王雅春, 陈幼春, 等.五种微卫星DNA标记在肉牛群体中的研究[J].中国农业科学, 1999, 1:69-73. CAO H H, WANG Y C, CHEN Y C, et al.Study on five microsatellite markers in beef cattle population[J].Scientia Agricultura Sinica, 1999, 1:69-73.(in Chinese)
[21] 马丽娜, 李颖康, 于洋, 等.滩羊肌肉抑制素(MSTN)基因的多态性研究[J].黑龙江畜牧兽医, 2015, 1:64-66. MA L N, LI Y K, YU Y, et al.Polymorphism study on myostatin (MSTN) gene in Tan sheep[J].Heilongjiang Animal Husbandry and Veterinary Medicine, 2015, 1:64-66.(in Chinese)
[22] 解彪, 张乃锋, 崔凯, 等.不同中性洗涤纤维水平饲粮对早期断奶羔羊生长性能、血清指标、屠宰性能和组织器官发育的影响[J].动物营养学报, 2018, 30(3):963-972. XIE B, ZHANG N F, CUI K, et al.Effects of different levels of dietary neutral detergent fiber on growth performance, serum parameters, slaughter performance and tissue and organ development of early-weaned Hu lambs[J].Chinese Journal of Animal Nutrition, 2018, 30(3):963-972.(in Chinese)
[23] 高林青, 占今舜, 胡耀, 等.不同精粗比全混合日粮对湖羊生长性能、血清激素浓度和屠宰性能的影响[J].动物营养学报, 2019, 31(4):1676-1684. GAO L Q, ZHAN J S, HU Y, et al.Effects of total mixed ration with different concentration roughage ratios on growth performance, serum hormone concentrations and slaughter performance of Hu sheep[J].Chinese Journal of Animal Nutrition, 2019, 31(4):1676-1684.(in Chinese)
[24] 郑莉芳, 陈佩杰, 肖卫华.骨骼肌质量控制信号通路[J].生理学报, 2019, 71(4):671-679. ZHENG L F, CHEN P J, XIAO W H.Signaling pathways controlling skeletal muscle mass[J].Journal of Physiology, 2019, 71(4):671-679.(in Chinese)
[25] GROCHOWSKA E, BORYS B, LISIAK D, et al.Genotypic and allelic effects of the myostatin gene (MSTN) on carcass, meat quality, and biometric traits in Colored Polish Merino sheep[J].Meat Science, 2019, 151:4-17.
[26] 张磊, 李丹妮, 王钰晨, 等.东佛里生羊MSTN基因多态性及其与生长性状的关联分析[J].中国畜牧兽医, 2021, 48(12):4537-4544. ZHANG L, LI D N, WANG Y C, et al.Analysis of MSTN gene polymorphism and its association with growth traits in East Friesian sheep[J].China Animal Husbandry & Veterinary Medicine, 2021, 48(12):4537-4544.(in Chinese)
[27] 郭振刚, 张立春, 曹阳, 等.中国草原红牛肌肉生长抑制素基因3'-UTR多态性及其与屠宰性状的关联性分析[J].中国畜牧兽医, 2013, 40(10):184-188. GUO Z G, ZHANG L C, CAO Y, et al.Analysis of the 3'-UTR polymorphism of muscle growth inhibitor gene and its association with slaughter traits in Chinese grassland Red bulls[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(10):184-188.(in Chinese)
[28] HAN J, FORREST R H, SEDCOLE J R, et al.Myostatin (MSTN) gene haplotypes and their association with growth and carcass traits in New Zealand Romney lambs[J].Small Ruminant Research, 2015, 127:8-19.
[29] 殷雨洋, 蒋永清, 黄杰, 等.青贮饲用油菜对湖羊生长性能、屠宰性能、瘤胃发酵及器官发育的影响[J].动物营养学报, 2021, 33(2):1153-1162. YIN Y Y, JIANG Y Q, HUANG J, et al.Effects of silage feeding rape on growth performance, slaughter performance, rumen fermentation and organ development of Hu sheep[J].Chinese Journal of Animal Nutrition, 2021, 33(2):1153-1162.(in Chinese)
[30] VAN DE PERRE V, PERMENTIER L, DE BIE S, et al.Effect of unloading, lairage, pig handling, stunning and season on pH of pork[J].Meat Science, 2010, 86(4):931-937.
[31] 闫金玲, 李蓓蓓, 李妍, 等.低蛋白质饲粮补充过瘤胃赖氨酸和过瘤胃蛋氨酸对荷斯坦公牛生长性能、屠宰性能、肉品质及氮代谢的影响[J].动物营养学报, 2022, 34(2):1014-1026. YAN J L, LI B B, LI Y, et al.Effects of low protein diet supplemented with rumen-protected lysine and rumen-protected methionine on growth performance, slaughter performance, meat quality and nitrogen metabolism of Holstein bulls[J].Chinese Journal of Animal Nutrition, 2022, 34(2):1014-1026.(in Chinese)
[32] WATANABE A, DALY C C, DEVINE C E.The effects of the ultimate pH of meat on tenderness changes during ageing[J].Meat Science, 1996, 42(1):67-78.
[33] 孙伟.关于绵羊肌肉生长遗传调控机理的研究[D].北京:中国农业科学院, 2012. SUN W.Study on the genetic regulation mechanism of muscle growth in sheep[D].Beijing:Chinese Academy of Agricultural Sciences, 2012.(in Chinese)
[34] GAO K, LUO Z, HAN S, et al.Analysis of meat color, meat tenderness and fatty acid composition of meat in second filial hybrid offspring of MSTN mutant pigs[J].Meat Science, 2022, 193:108929.
[35] KOBOLÁK, GÓCZA E.The role of the myostatin protein in meat quality-A review[J]. Archivfur Tierzucht, 2002, 45(2):159-170.
[36] ARSHAD M S, SOHAIB M, AHMAD R S, et al.Ruminant meat flavor influenced by different factors with special reference to fatty acids[J].Lipids in Health & Disease, 2018, 17(1):223.
[37] SAÑUDO C, ENSER M E, CAMPO M M, et al.Fatty acid composition and sensory characteristics of lamb carcasses from Britain and Spain[J]. Meat Science, 2000, 54(4):339-346.
[38] 双金, 敖力格日玛, 敖长金.苏尼特羊体脂脂肪酸组成的研究[J].畜牧兽医学报, 2015, 46(8):1363-1374. SHUANG J, AOLIGERIMA, AO C J.Study on composition of Sunit sheep body fatty acid[J]. Journal of Animal Husbandry and Veterinary Medicine, 2015, 46(8):1363-1374.(in Chinese)
[39] GARCIA P T, PENSEL N A, SANCHO A M, et al.Beef lipids in relation to animal breed and nutrition in Argentina[J].Meat Science, 2008, 79(3):500-508.
[40] ZHANG Y, ZHANG J, GONG H, et al.Genetic correlation of fatty acid composition with growth, carcass, fat deposition and meat quality traits based on GWAS data in six pig populations[J].Meat Science, 2019, 150:47-55.
[41] IWATA N G, PHAM M, RIZZO N O, et al.Trans fatty acids induce vascular inflammation and reduce vascular nitric oxide production in endothelial cells[J].PLoS One, 2011, 6(12):e29600.

Polymorphism of MSTN Gene and Its Association Analysis with Meat Quality Traits in Sheep

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