苏姜猪不同生长阶段背膘组织差异表达基因及其调控通路分析

doi:10.16431/j.cnki.1671-7236.2023.07.001

Abstract

Abstract: 【Objective】 The aim of this study was to compare the difference of gene expression profile during the developmental process of the backfat tissue in Sujiang pigs, and explore the key genes affecting the backfat tissue development.【Method】 The backfat tissue in Sujiang pigs at the age of 1, 3, 6 and 8 months (M1, M3, M6 and M8) was selected for high-throughput transcriptome sequencing, and the differentially expressed genes (DEGs) were screened for functional enrichment and expression trend analysis.【Result】 Transcriptome sequencing results showed that 1 869, 296 and 1 257 DEGs were identified in M1 vs M3, M3 vs M6 and M6 vs M8 groups, respectively.Functional enrichment showed that Wnt signaling pathway and ECM-receptor interaction were activated in M1 vs M3 group, while PI3K-Akt signaling pathway, AMPK signaling pathway, butanoate metabolism and citrate cycle (TCA cycle) were activated in M6 vs M8 group.Gene expression trend analysis showed that secreted protein, acid and rich in cysteine (SPARC), recombinant cathepsin S (CTSS), Kruppel-like factor 7 (KLF7) and other genes related to cell proliferation and development were highly expressed at 3 months old, while insulin-like growth factor 1 receptor (IGF1R), signal transducer and activator of transcription 1 (STAT1), fatty acid synthase (FASN) and other genes related to lipid metabolism were significantly expressed after 6 months old.【Conclusion】 This study obtained 4 gene expression profiles of backfat tissue in Sujiang pigs at different growth stages, the key genes related to cell proliferation and development were obtained, including SPARC, CTSS and KLF7, the key genes related to cellular lipid metabolism, including IGF1R, STAT1 and FASN, which provided basic data for genetic improvement of backfat thickness in Sujiang pigs.

Key words: Sujiang pigs; transcriptome sequencing; backfat tissue; differentially expressed genes

CLC Number:

Q786

NI Ligang, ZHOU Chunbao, XU Pan, ZHANG Yaqin, TAO Yong, ZHANG Junsheng. Analysis of Differentially Expressed Genes and Regulation Pathway of Backfat Tissue in Sujiang Pigs at Different Growth Stages[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2595-2605.

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Analysis of Differentially Expressed Genes and Regulation Pathway of Backfat Tissue in Sujiang Pigs at Different Growth Stages

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