基于转录组测序筛选疆南绒山羊次级毛囊周期发育相关的circRNA

doi:10.16431/j.cnki.1671-7236.2024.05.001

Abstract

Abstract: 【Objective】 This study was aimed to investigate the mechanism of growth and development in skin hair follicles of Jiangnan cashmere goats,enhance comprehension of the genetic regulation and function of candidate genes related to secondary hair follicle development,and provide guidance for future breeding of Jiangnan cashmere goats to improve their fleece production.【Method】 The high-throughput sequencing technology was used to carry out transcriptomic analysis of circular RNA (circRNA) in the skin tissues of secondary hair follicles in Jiangnan cashmere goats during anagen,catagen and telogen phases.The differentially expressed circRNA (DE circRNA) in different comparison groups was selected,and the host genes were analyzed by GO function,KEGG pathway and protein-protein interaction (PPI).The competitive endogenous RNA (ceRNA) network was constructed by combining the preliminary transcriptome data.【Result】 A total of 2 482 circRNAs were identified in 9 skin tissues of Jiangnan cashmere goats,of which 138 were DE circRNAs.In the comparison group of phases,there were 54,74 and 65 DE circRNAs were found in the comparison group of catagen and anagen,catagen and telogen,telogen and anagen phases,respectively.GO function and KEGG pathway analysis revealed that DE circRNA host genes were mainly concentrated in functional pathways such as cell adhesion,integrin-mediated signaling pathway,cell integrin complex,and some signaling pathways related to physiological processes of hair follicles such as MAPK,TGF-beta and Hedgehog.Combining with the PPI network,it was speculated that 14 circRNAs might participate in the regulation of growth and development of secondary hair follicles in Jiangnan cashmere goats.In addition,6 DE circRNAs,20 DE miRNAs,3 DE mRNAs and 2 DE lncRNAs were constructed in the ceRNA network of Jiangnan cashmere goats.【Conclusion】 In this study,transcriptome sequencing was used to screen essential circRNAs related to secondary hair follicle cycle development in Jiangnan cashmere goats,including novel-gene5238-2,novel-gene7855-1,novel-gene4455-1,etc.The results provided theoretical foundation for the biological function and regulatory property of circRNA in the development of secondary hair follicles in cashmere goats,and provided a new prospect for breeding of Jiangnan cashmere goats.

Key words: Jiangnan cashmere goats; transcriptome sequencing; circular RNA (circRNA); secondary hair follicle cycle

CLC Number:

S827
Q78

LU Qingwei, TANG Sen, LI Bin, QIN Chongkai, LIU Wenna, PU Xue, WANG Yaqian, WU Cuiling, FU Xuefeng. Screening of circRNA Related to Secondary Hair Follicle Cycle Development in Jiangnan Cashmere Goats Based on Transcriptome Sequencing[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 1793-1806.

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Screening of circRNA Related to Secondary Hair Follicle Cycle Development in Jiangnan Cashmere Goats Based on Transcriptome Sequencing

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