2株重组禽传染性支气管炎病毒的分离鉴定及基因组序列分析

doi:10.16431/j.cnki.1671-7236.2024.09.024

Abstract

Abstract: 【Objective】 The experiment was aimed to understand the genetic variation of avian Infectious bronchitis virus (IBV). 【Method】 Samples suspected to be infected with IBV was collected from partial farms in Shandong, inoculated with SPF chicken embryos for virus isolation and identification.Whole genome sequencing was performed using second-generation sequencing technology, and genetic evolution and key amino acid sites were analyzed based on the whole genome sequence. 【Result】 Autopsy of infected chickens showed hemorrhages in the tracheal rings and yellow or black-yellow embolism in the lumen.Two strains of IBV were isolated from the samples, and named SDJN and SDLY, respectively.Similarity analysis showed that isolates SDJN and SDLY had high similarity with GⅠ-19 genotype reference strains, with nucleotide similarity of 96.0%-97.3% and amino acid similarity of 94.9%-100%.Amino acid sequence analysis showed that the two IBV isolates had multiple amino acid variation sites compared with other strains.Phylogenetic tree results showed that isolates SDJN and SDLY were located in the same branch as the reference strains of the IBV GⅠ-19 genotype.Recombinant analysis showed that isolates SDJN and SDLY were recombinant strains of multiple genotypic strains, both with the ck/CH/LDL/091022 strain as the main parental strain. 【Conclusion】 In this study, two IBV isolates were isolated from Shandong province.The two isolates were closely related to GⅠ-19 genotype, and were a new recombinant IBV strain.The results laid a foundation for further study on the prevalence and evolution of IBV and the prevention and control of avian infectious bronchitis.

Key words: avain Infectious bronchitis virus; isolation; identification; whole genome; genetic evolutionary analysis

CLC Number:

S855.3

LI Feng, XIE Bin, ZHANG Shuai. Isolation，Identification and Genome Sequence Analysis of Two Recombinant Strains of Avian Infectious Bronchitis Virus[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(9): 3958-3969.

References

[1] GHETAS A M.Infectious bronchitis virus genotypes in the middle east[J].Avian Diseases,2021,65(4):647-651.
[2] 岳红艳.鸡传染性支气管炎的流行特点、诊断及防控[J].甘肃畜牧兽医,2023,53(6):40-42. YUE H Y.Epidemiological characteristics,diagnosis,prevention and control of infectious bronchitis in chickens[J].Gansu Animal Husbandry and Veterinary Medicine,2023,53(6):40-42.(in Chinese)
[3] 张春阳,王贵升,郑孟加,等.鸡传染性支气管炎病毒遗传演化最新研究进展[J].家禽科学,2023,45(11):61-66. ZHANG C Y,WANG G S,ZHENG M J,et al.Recent advances in the genetic evolution of chicken Infectious bronchitis virus[J].Poultry Science,2023,45(11):61-66.(in Chinese)
[4] 颜瑞娟.鸡传染性支气管炎的临床症状、病理变化及诊断方法[J].中国动物保健,2021,23(10):58-59. YAN R J.Clinical symptoms,pathologic changes and diagnosis of infectious bronchitis in chickens[J].China Animal Health,2021,23(10):58-59.(in Chinese)
[5] 李彬,韩晓青,刘红祥,等.2019—2021年我国29个省级行政区鸡传染性支气管炎流行病学调查[J].中国动物检疫,2022,39(4):19-25. LI B,HAN X Q,LIU H X,et al.Epidemiologic survey of chicken infectious bronchitis in 29 provincial-level administrative regions of China,2019-2021[J].China Animal Health Inspection,2022,39(4):19-25.(in Chinese)
[6] KEEP S,DOWGIER G,LULLA V,et al.Deletion of the s2 mRNA structure in the avian Coronavirus Infectious bronchitis virus and human Astrovirus results in sequence insertions[J].Journal of Virology,2023,97(3):e3823.
[7] QIN Y,TU K,TENG Q,et al.Identification of novel T-cell epitopes on Infectious bronchitis virus N protein and development of a multi-epitope vaccine[J].Journal of Virology,2021,95(17):e66721.
[8] BROWNSWORD M J,DOYLE N,BROCARD M,et al.Infectious bronchitis virus regulates cellular stress granule signaling[J].Viruses,2020,12(5):536.
[9] QUINTEROS J A,NOORMOHAMMADI A H,LEE S W,et al.Genomics and pathogenesis of the avian Coronavirus Infectious bronchitis virus[J].Australian Veterinary Journal,2022,100(10):496-512.
[10] ZHAO J,ZHAO Y,ZHANG G.Key aspects of Coronavirus Avian infectious bronchitis virus[J].Pathogens,2023,12(5):698.
[11] COLLISSON E W,PEI J,DZIELAWA J,et al.Cytotoxic T lymphocytes are critical in the control of Infectious bronchitis virus in poultry[J].Developmental Comparative Immunology,2000,24(2-3):187-200.
[12] ALONSO-HEARN M,BALLESTEROS A,NAVARRO A,et al.Lateral-flow assays for bovine paratuberculosis diagnosis[J].Frontiers in Veterinary Science,2023,10:1257488.
[13] ZEGPI R A,JOINER K S,VAN SANTEN V L,et al.Infectious bronchitis virus population structure defines immune response and protection[J].Avian Diseases,2020,64(1):60-68.
[14] GONZALES-VIERA O,CROSSLEY B,CARVALLO-CHAIGNEAU F R,et al.Infectious bronchitis virus prevalence,characterization,and strain identification in California backyard chickens[J].Avian Diseases,2021,65(1):188-197.
[15] MONDAL S P,LUCIO-MARTINEZ B,NAQI S A.Isolation and characterization of a novel antigenic subtype of Infectious bronchitis virus serotype DE072[J].Avian Diseases,2001,45(4):1054-1059.
[16] HODGSON T,CASAIS R,DOVE B,et al.Recombinant Infectious bronchitis coronavirus Beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity[J].Journal of Virology,2004,78(24):13804-13811.
[17] ABOZEID H H,NAGUIB M M.Infectious bronchitis virus in egypt:Genetic diversity and vaccination strategies[J].Veterinary Sciences,2020,7(4):204.
[18] 陈静.鸡传染性支气管炎的流行现状与防控措施[J].北方牧业,2024，3:23. CHEN J.Epidemiological status and preventive and control measures of infectious bronchitis in chickens[J].Northern Pastoralism,2024,3:23.(in Chinese)
[19] TANG Y,LU H.Genomic characterization of a Broiler reovirus field strain detected in Pennsylvania[J].Infection Genetics Evolution,2015,31:177-182.
[20] TENG L,XIE Z,XIE L,et al.Sequencing and phylogenetic analysis of an Avian reovirus genome[J].Virus Genes,2014,48(2):381-386.
[21] HOU Y,ZHANG L,REN M,et al.A highly pathogenic GⅠ-19 lineage Infectious bronchitis virus originated from multiple recombination events with broad tissue tropism[J].Virus Research,2020,285:198002.
[22] WANG C,HOU B.A pathogenic and recombinant Infectious bronchitis virus variant (CK/CH/GX/202109) with multiorgan tropism[J].Veterinary Research,2023,54(1):54.
[23] XU L,REN M,SHENG J,et al.Genetic and biological characteristics of four novel recombinant avian Infectious bronchitis viruses isolated in China[J].Virus Research,2019,263:87-97.
[24] YUAN W,LV T,JIANG W,et al.Antigenic characterization of Infectious bronchitis virus in the South China during 2021-2022[J].Viruses,2023,15(6):1273.
[25] HUANG M,ZOU C,LIU Y,et al.A novel low virulent respiratory Infectious bronchitis virus originating from the recombination of QX,TW and 4/91 genotype strains in China[J].Veterinary Microbiology,2020,242:108579.
[26] YAN W,YANG Q,HUANG S,et al.Insights on genetic characterization and pathogenesis of a GⅠ-19(QX-like) Infectious bronchitis virus isolated in China[J].Poultry Science,2023,102(7):102719.
[27] QI X,CHEN Y,REN X,et al.A reassortment vaccine candidate as the improved formulation to induce protection against very virulent Infectious bursal disease virus[J].Vaccine,2014,32(13):1436-1443.

Isolation，Identification and Genome Sequence Analysis of Two Recombinant Strains of Avian Infectious Bronchitis Virus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WU Jingnan, LI Zhanhong, LI Fuxiang, ZHANG Zhenxing, ZHANG Yifang, SONG Jianling. Isolation,Identification and Biological Characterization Analysis of Phage BP32 from Multidrug-Resistant Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3540-3551.
[2]	XU Fei, WEN Guilan, GONG Xinyong, WEN Ming, CHEN Bofan. Isolation and Identification of a Multidrug-resistant Strain of Enterococcus faecalis from Dog and Analysis of Its Pathogenicity [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3615-3624.
[3]	ZHANG Yan, WEI Zhitong, HU Yehao, ZHANG Huaju, ZHANG Man, FU Tong, LIU Xian, LI Zhigang, QI Xinglei, WANG Eryao, ZHANG Zijing, LIANG Dong, GAO Tengyun. Genetic Diversity and Population Structure Analysis of Jiaxian Red Cattle Based on Whole Genome Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2933-2942.
[4]	ZHAO Bilig, WEN Xin, YI Minna, Tseweendolmaa, SHEN Yingchao, SI Qin, Zhaolunqiqig, REN Hong, MANG Lai, Gerelchimeg. Isolation and Culture of Equine Umbilical Vein Endothelial Cells and Their Functional Validation [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2953-2962.
[5]	ZHANG Xiaoyong, LAN Wei, WANG Ya, LAO Jianlong, LI Zhi, JIA Dan, KONG Xiangying, HU Kaiyin, WEN Yongsen, LYU Shuting, CHEN Man, YIN Hong, LI Youquan. Isolation,Identification and Antimicrobial Properties of Enterococcus faecium FDT2 [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3020-3029.
[6]	JIAO Fengchao, LEI Zhen, LI Yingxiao, WU Xian, QU Zhehui. Whole Genome Sequencing and Virulence and Drug Resistance Analysis of a Strain of Proteus mirabilis Derived from Peacock [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3030-3040.
[7]	LIU Wenkai, WANG Ping, JIN Yinghong, XUE Jing, LI Yue, LIANG Qianqian, LI Xiaozhuo, HAN Xiangshu, ZHENG Qiming, JIANG Song, XIA Jun. Isolation,Identification and Genetic Evolution Analysis of a Strain of Bovine Coronavirus [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3100-3108.
[8]	ZHAO Yiduo, ZHANG Kang, WANG Lei, LU Xiaorong, ZHANG Jingyan, CHENG Jia, GUO Zhiting, XU Guowei, HUANG Liping, NIU Yuhui, MA Ting, FENG Xiaowei, EGIDE, LIU Mingchao, LI Jianxi. Isolation,Identification and Pathogen Characteristics Evaluation of a Strain of Pasteurella multocida D:L3:L5 from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3128-3141.
[9]	SU Hui, WANG Yunfei, ZHAO Xinling, LIU Mimi, ZOU Mingxiao, DONG Wenlong, LI Guojiang, YIN Baishuang. Physicochemical Properties Research of Lactiplantibacillus plantarum MU-6 Bacteriocin and Its Coding Gene Mining [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3166-3175.
[10]	CHEN Yixing, ZHANG Meilin, LI Shikai, YANG Zhican, LI Wanjia, CHEN Jidang, ZHU Wanjun, ZHANG Jipei. Isolation,Identification and Drug Resistance Analysis of Riemerella anatipestifer from Some Areas in Guangdong [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3185-3194.
[11]	YUAN Huijun, YU Shiman, XU Yanying, YUAN Yijun, FENG Huan, ZHANG Huanhuan. Identification and Bacteriostatic Activity Analysis of an Antifungal Bacillus velezensis lut-Y1 [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2354-2364.
[12]	WANG Zi, SUN Miao, WANG Yongqiang, MENG Linghao, GENG Chao, SHI Jinchuan, CHEN Wei, QIU Jun, LIU Kai. Detection of Drug Resistance of Escherichia coli from Ducks in Some Areas of Tongliao and Whole Genome Sequencing Analysis of Multi-drug Resistant Strain YA-1 [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2585-2596.
[13]	YU Fuxin, WU Siyu, YU Jieshi, ZHANG Xiaoai, YU Ting, DONG Bo, LI Jianbo, WANG Lei, WEI Wenkang. Isolation,Identification,Whole Genome Sequencing and Genetic Evolution Analysis of Goose Astrovirus in Some Areas of Guangdong Province [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2633-2643.
[14]	WU Yuxing, ZOU Weili, CHENG Zixin, MO Yupeng, ZHANG Lingyuan, JI Wantong, CHEN Siyu, ZHENG Haodong, MA Runwen, LI Xun, LI Maoning, WANG Xiaoye. Isolation, Identification and Pathogenicity Analysis of a Strain of Swine-derived Clostridium perfringens Type A [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2697-2706.
[15]	DU Dongdong, QIAN Jing, CHANG Hengrui, LI Hongmin, WEI Xiangli, LIU Changyong, LUO Ruifeng, WANG Guohong, KANG Lichao. Whole Genome Sequencing Analysis of a ST515 Strain of Multidrug-resistant Listeria monocytogenes [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2707-2716.