屎肠球菌FDT2的分离鉴定及抑菌特性研究

doi:10.16431/j.cnki.1671-7236.2024.07.027

Abstract

Abstract: 【Objective】 The purpose of this experiment was to isolate and identify Enterococcus faeciums isolates from yak faeces and conduct in vitro antibacterial studies,in order to screen probiotics with excellent antibacterial properties.【Method】 In this experiment,Enterococcus spp.were isolated from faeces of healthy yaks in Qinghai province utilizing MRS medium,the strains were identified by combining morphology and molecular biology and conduct antibacterial research.The supernatants of the strains were isolated by fermentation filtration,and the corresponding antibacterial effect was observed using punching method.The antibacterial substances were analyzed by changing pH,adding catalase,pepsin and trypsin,and the effects of temperature and pH on the fermentation supernatants were explored.【Result】 The results of morphology and phylogenetic tree analysis showed that the two new isolates (2 and D2) belonged to Enterococcus faecium. The antibacterial experiment results showed that Enterococcus faecium FDT2 had inhibitory effect on all five pathogenic bacteria,and the inhibitory effects on enterotoxigenic Escherichia coli K88 and Escherichia coli ATCC 43888 were more stronger,while D2 had no inhibitory effect on all five pathogenic bacteria.The acid excretion test results proved that the acidic substance in the fermentation supernatant of Enterococcus faecium FDT2 did not have antibacterial effects.The experiment of adding catalase proved that hydrogen peroxide existed in the bacteriostatic substance and played partly bacteriostatic effect.After addition of pepsin and trypsin,it was found that the diameter of inhibition zone was extremely significantly lower than that of control group (P＜0.01),indicating that antibacterial substances contained bacteriocin and played a major role in antibacterial effects.With the gradual increase of temperature,the antibacterial activity of Enterococcus faecium FDT2 decreased,and it remained antibacterial activity at 100 ℃,indicating its strong thermal stability.With the increase of pH,the bacteriostatic activity of Enterococcus faecium FDT2 decreased gradually,which also indicated that bacteriocin had strong activity in acidic environment.【Conclusion】 Two strains of bacteria were isolated in the experiment,among which Enterococcus faecium FDT2 had good bacteriostatic properties and contained bacteriocin and other antibacterial substances,and it was necessary to further study on the bacteriocin of Enterococcus faecium FDT2.

Key words: Enterococcus faecium; identification; morphology; antimicrobial activity

CLC Number:

S852.61⁺1

ZHANG Xiaoyong, LAN Wei, WANG Ya, LAO Jianlong, LI Zhi, JIA Dan, KONG Xiangying, HU Kaiyin, WEN Yongsen, LYU Shuting, CHEN Man, YIN Hong, LI Youquan. Isolation,Identification and Antimicrobial Properties of Enterococcus faecium FDT2[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 3020-3029.

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Isolation,Identification and Antimicrobial Properties of Enterococcus faecium FDT2

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