一种外三元纯种猪、二元猪、三元猪和多元回交猪的鉴定方法研究

doi:10.16431/j.cnki.1671-7236.2024.03.028

Abstract

Abstract: 【Objective】 The purpose of this experiment was to obtain the effective genotypes for the identification of foreign ternary pig breeds and to establish a systematic identification scheme for the identification of foreign ternary pig germplasm.【Method】 In this study,homozygous loci of 613 Duroc pigs Illumina 60K chip,503 Landrace pigs Illumina core No.1 50K chip and 10 Yarkshire pigs resequencing data were statistically analyzed,and specific single nucleotide polymorphism (SNP) of Duroc,Landrace and Yarkshire pigs were screened.Ear tissue DNA of 120 pigs with different strains and varieties was used as template for PCR amplification and sequencing to verify the specific SNP,and the effective specific gene loci were screened.The identification schemes of foreign ternary purebred,binary,ternary and multiple backcross pigs were established through loci combination analysis.【Result】 A total of 6 SNPs specific to Landrace pigs,8 SNPs specific to Duroc pigs and 1 SNP specific to Yarkshire pigs were obtained by comparison of chip data.The effective SNPs were SSC13:57293969 A＞G,SSC11:47494227 T＞G,SSC6:110653413 T>C and SSC6:110653219 T＞C,respectively.According to the mutation types and genetic rules of the loci,the germplasm identification scheme of three-loci joint analysis was developed,and several pig breeds such as Landrace pure breed pigs and multi-backcross Landrace pigs were identified.【Conclusion】 In this study, the identification schemes of foreign ternary pure breed,binary,ternary and multiplex backcross pig breeds was developed by pig-specific SNP.The results provided reference and technical support for germplasm identification of foreign ternary pigs.

Key words: foreign ternary pig; chip comparison; single nucleotide polymorphism (SNP); germplasm identification

CLC Number:

S813.3

ZHENG Chunting, HU Yunyang, ZHANG Chunguang, YE Ziyuan, ZHANG Chen, LUO Yunyan, QIN Lei, PENG Zixuan, YU Yifei, XIAN Wangying, CHU Han, HONG Liang, ZHANG Jianbin, PU Lei. Research on Identification Method of Foreign Ternary Purebred, Binary,Ternary and Multiple Backcross Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1160-1170.

References

[1] 杨厚德.猪育种存在的问题及育种方式的选择[J].湖北畜牧兽医, 2020, 41(11):28-29. YANG H D.Problems in pig breeding and selection of breeding methods[J].Hubei Journal of Animal and Veterinary Sciences, 2020, 41(11):28-29.(in Chinese)
[2] 张利,梁文载,梁桂超,等.非洲猪瘟对我国生猪生产和市场行情的影响[J].中国猪业, 2020, 15(3):18-23. ZHANG L, LIANG W Z, LIANG G C, et al.Impact of African swine fever on China's pig production and market conditions[J].China Swine Industry, 2020, 15(3):18-23.(in Chinese)
[3] BERNAERDT E, DEWULF J, VERHULST R, et al.Purchasing policy, quarantine and acclimation practices of breeding gilts in Belgian pig farms[J]. Porcine Health Management, 2021, 7(1):25.
[4] WANG G, WANG J, CHEN S, et al.Vertical integration selection of Chinese pig industry chain under African swine fever-From the perspective of stable pig supply[J].PLoS One, 2023, 18(2):e0280626.
[5] CHENG J, WARD M P.Risk factors for the spread of African swine fever in China:A systematic review of Chinese-language literature[J].Transboundary and Emerging Diseases, 2022, 69(5):e1289.
[6] 孙齐田,刘海霞.非洲猪瘟时期对猪育种工作的影响及发展措施[J].中国动物保健, 2023, 25(3):85-86. SUN Q T, LIU H X.Impact of the African swine fever period on pig breeding and development measures[J].China Animal Health, 2023, 25(3):85-86.(in Chinese)
[7] 唐会平.非洲猪瘟时期对猪育种工作的影响及发展措施[J].畜牧兽医科学(电子版), 2021, 87(3):172-173. TANG H P.Influence of African swine fever period on pig breeding and development measures[J].Graziery Veterinary Sciences (Electronic Version), 2021, 87(3):172-173.(in Chinese)
[8] HUANG Y, LI J, ZHANG J, et al.Dynamical analysis of the spread of African swine fever with the live pig price in China[J]. Mathematical Biosciences and Engineering, 2021, 18(6):8123-8148.
[9] 胡雄贵,陈晨,蔡文杰.三元商品猪留种做母猪的注意事项[J].湖南畜牧兽医, 2019, 214(6):3-5. HU X G, CHEN C, CAI W J.Considerations for saintyear commercial hogs to be kept as sows[J].Hunan Journal of Animal Science&Veterinary Medicine, 2019, 214(6):3-5.(in Chinese)
[10] 牛安然,张兴,杨雨婷,等.基于SNP芯片分析鉴定大白猪、长白猪与杜洛克猪的品种成分[J].中国畜牧杂志, 2022, 58(8):219-223. NIU A R, ZHANG X, YANG Y T, et al.Identification of breed components of Yarkshire, Landrace and Duroc pigs based on SNP microarray analysis[J].Chinese Journal of Animal Science, 2022, 58(8):219-223.(in Chinese)
[11] HUANG M, YANG B, CHEN H, et al.The fine-scale genetic structure and selection signals of Chinese indigenous pigs[J].EvolutionaryApplications, 2019, 13(2):458-475.
[12] 赵亮,陶红军.我国种猪产业发展与市场结构判断[J].养猪, 2021, 179(6):1-8. ZHAO L, TAO H J.Development of China's pig breeding industry and market structure judgment[J].Swine Production, 2021, 179(6):1-8.(in Chinese)
[13] 黄进杰.基于SNP芯片利用不同方法鉴定个体猪品种[D].广州:华南农业大学, 2019. HUANG J J.Identify pig breeds with different methods based on SNP chip[D].Guangzhou:South China Agricultural University, 2019.(in Chinese)
[14] 于辉,李建东,赵海全,等.三品种外种猪SLA-DQA基因的PCR-RFLP分析[J].中国畜牧杂志, 2010, 46(9):14-17. YU H, LI J D, ZHAO H Q, et al.PCR-RFLP analysis of SLA-DQA gene in three outbred pig breeds[J].Chinese Journal of Animal Science, 2010, 46(9):14-17.(in Chinese)
[15] GAO J, SUN L, ZHANG S, et al.Screening discriminating SNPs for Chinese indigenous pig breeds identification using a random forests algorithm[J].Genes&Development, 2022, 13(12):2207.
[16] 王鲁,章瑞,陈立平,等.种用外三元母猪的选留策略[J].浙江畜牧兽医, 2021, 46(5):43-44. WANG L, ZHANG R, CHEN L P, et al.Selection and retention strategies for breeding sows with external ternary sows[J].Zhejiang Journal Animal Science and Veterinary Medicine, 2021, 46(5):43-44.(in Chinese)
[17] CAI Z, SARUP P, OSTERSEN T, et al.Genomic diversity revealed by whole-genome sequencing in three Danish commercial pig breeds[J]. Journal of Animal Science and Biotechnology, 2020, 98(7):skaa229.
[18] 颜国华,杨玉增,张秋良,等.不同品系长白、大白、杜洛克种猪种质特征及生产性能比较分析[J].北方牧业, 2019, 592(24):16-17. YAN G H, YANG Y Z, ZHANG Q L.et al.Comparative analysis of germplasm characteristics and production performance of different lines of Landrace, Yarkshire and Duroc breeding pigs[J].Northern Pastoralism, 2019, 592(24):16-17.(in Chinese)
[19] 杨晴,巩静,赵雪艳,等.芯片和重测序在猪遗传结构研究中的应用比较[J].畜牧兽医学报, 2023, 54(7):2772-2782. YANG Q, GONG J, ZHAO X Y.et al.Comparison of array and resequencing in pig genetic structure studies[J].Chinese Journal of Animal and Veterinary Sciences, 2023, 54(7):2772-2782.(in Chinese)
[20] TINARELLI S, RIBANI A, UTZERI V J, et al.Redefinition of the mora romagnola pig breed herd book standard based on DNA markers useful to authenticate its "Mono-Breed" products:An example of sustainable conservation of a livestock genetic resource[J].Animals, 2021, 11(2):526.
[21] KNOL E F, NIELSEN B, KNAP P W.Genomic selection incommercial pig breeding[J]. Animal Frontiers, 2016, 6(1):15-22.
[22] FAHRENKRUG S C, FREKING B A, SMITH T P, et al.Single nucleotide polymorphism (SNP) discovery in porcine expressed genes[J].Journal of Animal Breeding and Genetics, 2002, 33(3):186-195.
[23] 宋春雷,高灵琦.猪生产性状相关SNP位点研究进展[J].南方农业, 2020, 14(2):167-170. SONG C L, GAO L Q.Progress in the study of SNP loci related to pig production traits[J].Southern Agriculture, 2020, 14(2):167-170.(in Chinese)
[24] 顿桂玲,李祥龙,曹洪战,等.3个猪品种黑素皮质素受体1(MC1R)基因变异研究[J].遗传学报, 2007, 9:777-782. DUN G L, LI X L, CAO H Z, et al.Variations of melanocortin receptor 1(MC1R) gene in three pig breeds[J].Journal of Genetics and Genomics, 2007, 9:777-782.(in Chinese)
[25] WILMOT H, BORMANN J, SOYEURT H, et al.Development of a genomic tool for breed assignment by comparison of different classification models:Application to three local cattle breeds[J].Journal of Animal Breeding and Genetics, 2022, 139:40-61.
[26] XU Z T, DIAO S Q, TENG J Y, et al.Breed identification of meat using machine learning and breed tag SNPs[J].Food Control, 2021, 125:107971.
[27] 岳阳.基于单核苷酸多态性标记鉴别太湖流域地方品种猪及其制品的方法研究[D].上海:上海交通大学, 2019. YUE Y.Study on identification method of pigs in Taihu basin and their products based on single nucleotide polymorphism markers[D].Shanghai:Shanghai Jiao Tong University, 2019.(in Chinese)

Research on Identification Method of Foreign Ternary Purebred, Binary,Ternary and Multiple Backcross Pigs

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