大白猪PTGS2基因外显子2多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2018.01.018

Abstract

Abstract:

This study was aimed to investigate the association between single nucleotide polymorphisms (SNPs) of prostaglandin-endoperoxide synthase 2 (PTGS2) gene exon 2 and the reproductive traits in Large White pigs, which provided a method for the marker-assisted selection. DNA of American and France Large White sows were used to detect the single nucleotide mutation of PTGS2 gene by sequencing alignment, the genotype of polymorphic loci was identified by PCR-RFLP method, the association with reproductive traits in Large White pigs was analyzed. The results showed that a mutation site (g.86A>G) was found of PTGS2 gene exon 2, which changed MspⅠ restriction enzyme cutting site. Three genotypes of AA, AG and GG were detected in Large White pigs. The polymorphism distribution of PTGS2 gene g.86A>G fitted for the Hardy-Weinberg equilibrium in France Large White pigs (P>0.05). In American Large White pigs group, the number of weak piglets in primiparous sow with AA genotype was significantly lower than that of AG genotype (P<0.05), while the litter weight of multiparous sows with AA genotype was significantly higher than that of AG genotype (P<0.05). In France Large White pigs group, the total number born, number of strong piglets and litter weight of GG genotype were higher than those of AA and AG genotypes (P>0.05). The mutation site g.86A>G of PTGS2 gene exon 2 was significant association with the litter weight of multiparous sows and the number of weak piglets,so the polymorphic loci might be one of the candiate molecular markers used in pig breeding.

Key words: PTGS2 gene; Large White pigs; single nucleotide polymorphism (SNP); reproductive traits

CLC Number:

S813.3

NIE Guangwei, LI Mingyang, MAO Cui, MAO Huimin, WANG Guoshui, ZUO Bo. Polymorphism of PTGS2 Gene Exon 2 and Its Association Analysis with Reproductive Traits in Large White Pigs[J]. , 2018, 45(1): 140-146.

References

[1] LINVILLE R C, POMP D, JOHNSON R K, et al. Candidate gene analysis for loci affecting litter size and ovulation rate in swine[J].Journal of Animal Science, 2001,79(1):60-67.
[2] 王训翠,储明星,陈宏权. 前列腺素内过氧化物合酶2基因的研究进展[J]. 生命科学, 2004, 1:31-34. WANG X C, CHU M X, CHEN H Q. Advances on prostaglandin-endoperoxide synthase 2 gene[J]. Chinese Bulletin of Life Science, 2004, 1:31-34.(in Chinese)
[3] 宋桃伟, 罗卫星, 蔡惠芬, 等. 山羊前列腺素内过氧化物合酶2基因的多态性检测及生物信息学分析[J]. 中国畜牧兽医,2013,40(2):121-125. SONG T W, LUO W X, CAI H F, et al. Polymorphism dection and bioinformatics analysis of prostaglandin-endoperoxide synthase 2 gene in goat[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(2):121-125.(in Chinese)
[4] FRUNGIERI M B, WEIDINGER S, MEINEKE V, et al. Proliferative action of mast-cell tryptase is mediated by PAR2, COX2, prostaglandins, and PPARγ:Possible relevance to human fibrotic disorders[J]. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(23):15072-15077.
[5] HASE T, YOSHIMURA R, MATSUYAMA M, et al. Cyclooxygenase-1 and-2 in human testicular tumours[J].European Journal of Cancer, 2003, 39(14):2043-2049.
[6] GAMBERA L, SERAFINI F, MORGANTE G, et al. Sperm quality and pregnancy rate after COX-2 inhibitor therapy of infertile males with abacterial leukocytospermia[J]. Human Reproduction, 2007, 22(4):1047-1051.
[7] CÍRDOBA S, BALCELLS I, CASTELLÍ A, et al. Endometrial gene expression profile of pregnant sows with extreme phenotypes for reproductive efficiency[J].Scientific Reports, 2015, 5:14416-14431.
[8] LIM H, PARIA B C, DAS S K, et al. Multiple female reproductive failures in cyclooxygenase 2-deficient mice[J].Cell, 1997, 91(2):197-208.
[9] LINVILLE R C, POMP D, JOHNSON R K, et al. Candidate gene analysis for loci affecting litter size and ovulation rate in swine[J].Journal of Animal Science, 2001, 79(1):60-67.
[10] JOHNSON R K, NIELSEN M K, CASEY D S. Responses in ovulation rate, embryonal survival, and litter traits in swine to 14 generations of selection to increase litter size[J].Journal of Animal Science, 1999, 77(3):541-557.
[11] 王训翠, 储明星, 陈宏权, 等. 4个绵羊品种PTGS2基因部分片段的多态性分析[J]. 安徽农业大学学报,2008, 1:21-25. WANG X C,CHU M X,CHEN H Q. et al. Polymorphism analysis of partial fragment of PTGS2 gene in four sheep breeds[J]. Journal of Anhui Agricultural University, 2008, 1:21-25.(in Chinese)
[12] 雷彬. 大白猪和二花脸猪排卵前卵泡差异表达miRNAs及其靶基因鉴定[D]. 武汉:华中农业大学,2013. LEI B. Identification of differentially expressed miRNAs and their targets in the pre-ovulatory follicles of Large White and Erhualian sows[D].Wuhan:Huazhong Agricultural University,2013.(in Chinese)
[13] DING N S, REN D R, GUO Y M, et al. Genetic variation of porcine prostaglandin-endoperoxide synthase 2(PTGS2) gene and its association with reproductive traits in an Erhualian×Duroc F2 population[J]. Acta Genetica Sinica, 2006, 33(3):213-219.
[14] KORWIN-KOSSAKOWSKA A, KAPELANSKI W, BOCIAN M, et al. Preliminary study of the RBP4, EGF and PTGS2 genes polymorphism in pigs and its association with reproduction traits of sows[R]. Animal Science Papers & Reports, 2005.
[15] LU X, CHEN F, LIU X, et al. Detection and clinical significance of COX-2 gene SNPs in gastric cancer[J].Cell Biochemistry & Biophysics,2015,72(3):1-4.
[16] LEE C H, PAO J B, LU T L, et al. Prognostic value of prostaglandin-endoperoxide synthase 2 polymorphisms in prostate cancer recurrence after radical prostatectomy[J].International Journal of Medical Sciences, 2016, 13(9):696-700.
[17] WU G, CAI H, CAI H, et al. Influence of the cyclooxygenase-2 gene -765G/C and -1195G/A polymorphisms on development of ischemic stroke[J]. Journal of Stroke & Cerebrovascular Diseases, 2016, 25(9):2126-2135.
[18] GLADNEY C D, MARTINEZ V G, BRUMBAUGH K A, et al. Mapping of the prostaglandin-endoperoxide synthase 2(PTGS2) gene to porcine chromosome 9 and bovine chromosome 16 by linkage analysis using novel PCR-RFLP[J].Journal of Animal Science, 1999, 77(3):787-788.
[19] HOSSEIN K D. Identification and SNP detection for preimplantation active genes and their association with embryo development and male fertility in cattle[D]. Germany:Institute of Animal Science Animal Breeding and Husbandry,2007.
[20] KAEWMALA K, UDDIN M J, CINAR M U, et al. Investigation into association and expression of PLCz and COX-2 as candidate genes for boar sperm quality and fertility[J]. Reproduction in Domestic Animals,2012, 47(2):213-223.
[21] BIDANEL J P, ROSENDO A, IANNUCCELLI N, et al. Detection of quantitative trait loci for teat number and female reproductive traits in Meishan×Large White F2 pigs[J].Animal, 2008, 2(6):813-820.
[22] XING Y Y, REN J, REN D R, et al. A whole genome scanning for quantitative trait loci on traits related to sperm quality and ejaculation in pigs[J].Animal Reproduction Science, 2009, 114(1):210-218.
[23] SIRONEN A I, UIMARI P, SERENIUS T, et al. Effect of polymorphisms in candidate genes on reproduction traits in Finnish pig populations[J].Journal of Animal Science, 2010, 88(3):821-827.

Polymorphism of PTGS2 Gene Exon 2 and Its Association Analysis with Reproductive Traits in Large White Pigs

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