玻璃化冻存对驴卵母细胞超微结构的影响

doi:10.16431/j.cnki.1671-7236.2018.01.019

Abstract

Abstract:

This study was aimed to evaluate the effect of vitrification freezing on the development of oocytes in Equus asinus, and seek the best freezing conditions of oocytes in Equus asinus. The oocytes of Equus asinus in different development stages were vitrified and frozen, after the resuscitation, the mature culture and parthenogenetic activation were performed, respectively. The oocytes were stained with microfilaments and mitochondrial ultrastructure, and the oocytes were divided into GV stage without freezing group (control group), GV stage freezing group, IVM-M Ⅱ freezing group. The normal rate of oocyte morphology, maturation rate, the cleavage rate of partial activation, the normal rate of ultrastructural were counted. The results showed that there was no significant difference in the normal rate of morphology between GV stage freezing group and GV stage without freezing (P>0.05), and the maturation rate and cleavage rate were significantly lower than those in control group (P<0.05), the cleavage rate of IVM-M Ⅱ freezing group was significantly lower than that of control group (P<0.05), and the cell development was blocked after cleavage. The distribution of microfilaments in the cortex of most oocytes in freezing group was significantly reduced, and the number of mitochondria in freezing group was significantly lower than that in control group, so it could be explained that freezing caused damage to the oocyte's ultrastructure, resulting in decreased resuscitation after the maturity rate, affecting the oocyte fertilization and in vitro development, and the GV stage freezing group had less damage to microfilaments and mitochondrial structure than IVM-M Ⅱ freezing group, and the developmental status was better.

Key words: Equus asinus; oocyte; vitrification freezing; microfilaments; mitochondria

CLC Number:

S822.3

PANG Yujuan, DU Xingyu, LI Qiang, ZHANG Zhipeng, ZHANG Yanru, PAN Qingjie, CAO Junwei. Effects of Vitrification Freezing on Oocytes Ultrastructure in Equus asinus[J]. , 2018, 45(1): 147-153.

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Effects of Vitrification Freezing on Oocytes Ultrastructure in Equus asinus

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