华南虎源产碱普罗威登斯菌的分离鉴定及致病性研究

doi:10.16431/j.cnki.1671-7236.2024.03.044

Abstract

Abstract: 【Objective】 The aim of this experiment was to isolate and identify the pathogenic bacteria causing the acute death of South China tiger,determine its pathogenicity and drug sensitivity,and provide scientific basis for the prevention and control of the disease in the future.【Method】 Morphological and molecular biology were used to determine the types of isolated strains,and the pathogenicity test and drug sensitivity test of the isolated strains were carried out.【Result】 A strain of bacteria was isolated from the intestine of dead South China tiger cubs.The isolated bacteria showed gray-white colonies on the fresh blood agar plate and white colonies on the MacConkey plate.Microscopically,it was Gram-negative bacilli,named FJ/Tiger201802.Combined with the results of 16S rRNA sequencing,it was identified as Providencia alcalifaciens.Phylogenetic analysis showed that FJ/Tiger201802 was closely related to HXM38 (MZ734428.1) in the same evolutionary branch,and the nucleotide similarity of 16S rRNA gene was 99.5%.The mice inoculated with the isolated strain showed symptoms such as listlessness,loose hair,clumping,and secretions in the eyes.The median lethal dose (LD₅₀) of the isolated strain to mice was 1×10^8.2 CFU.The results of pathological anatomy and histopathology showed that the pathogenicity of FJ/Tiger201802 was strong,mainly affecting the liver,lung,intestine and other tissues and organs of mice.The results of drug sensitivity test showed that ampicillin,aztreonam,cefuroxime,cefoperazone,ceftriaxone,cefepime,ceftazidime,cefoxitin,ofloxacin,streptomycin,tobramycin and gentamicin had a strong inhibitory effect on the growth of the isolated strain in vitro,and the isolate were not sensitive to cefazolin and cefalotin.【Conclusion】 In this study,a strain of Providencia alcalifaciens from South China tiger was isolated,which could cause infection in mice.The results provided a reference for the clinical diagnosis and treatment of Providencia alcaligenes.

Key words: South China tiger; Providencia alcalifaciens; isolation and identification; pathogenicity

CLC Number:

S852.61

LI Yuqi, ZHU Zhihao, ZHENG Xin, XU Guizong, CAI Sisi, ZHOU Xianjing, JIN Yuxin, LIU Lu, XU Jingru, CHEN Fangting, XUE Mengru, KANG Yali, XUE Yuxi, CHEN Tengteng, LIN Xipan, LIN Kaixiong, TAN Congling, ZHANG Min, FAN Kewei. Isolation,Identification and Pathogenicity of Providencia alcalifaciens from South China Tiger[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1320-1328.

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Isolation,Identification and Pathogenicity of Providencia alcalifaciens from South China Tiger

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