三叶青藤叶粉对崇仁麻鸡生长性能、血清生化指标及空肠形态结构的影响

doi:10.16431/j.cnki.1671-7236.2024.06.022

Abstract

Abstract: 【Objective】 The aim of this study was to evaluate the effects of Tetrastigma hemsleyanum leaves powder (THL) on growth performance,serum antioxidant,immune function and jejunum morphology of Chongren patridge chickens.【Method】 A total of 360 50-day-old Chongren patridge female chickens were randomly divided into 5 groups with 6 replicates per group and 12 hens per replicate.Hens in control group were fed a basal diet,and hens in experimental groups were fed the basal diet supplemented with 0.5%,1%,2% and 4% THL,respectively.The experiment lasted for 56 days.After the completion of the experiment,weight was utilized as the repetition unit,and feed intake was recorded for calculating the growth performance index.Blood and intestinal tissue samples were collected,serum antioxidant and immune indexes were detected,jejunum tissue sections were prepared and jejunum morphology was observed.The villus height and crypt depth of jejunum were measured,and the V/C ratio was calculated.【Result】 Compared with control group,the ratio of feed to gain (F/G) of Chongren patridge chickens supplemented with 1% THL was significantly decreased (P ＜ 0.05),the contents of immunoglobulin A (IgA),IgM,transforming growth factor β (TGF-β) and the activity of glutathione peroxidase (GSH-Px) in serum were significantly increased (P＜0.05).The contents of tumor necrosis factor-α (TNF-α) and malondialdehyde (MDA) were significantly decreased (P＜0.05).The contents of IgA and TGF-β in serum were significantly increased (P＜0.05),and the contents of interleukin-1β (IL-1β) and TNF-α in serum were significantly decreased (P＜0.05) after adding 2% THL.The serum TGF-β content was significantly increased (P＜0.05),and the serum IL-1β content was significantly decreased (P＜0.05) after the supplementation of 4% THL.The villus height and V/C of jejunum were significantly increased after diets supplemented with 0.5% and 1% THL (P＜0.05).【Conclusion】 Dietary supplementation of 0.5% to 4% THL could promote the growth,enhance body immunity and improve intestinal tisste morphology of Chongren patridge chickens to a certain extent.Under the conditions of this experiment, the effect of 1% to 2% THL supplementation was better.

Key words: Tetrastigma hemsleyanum leaves powder; Chongren patridge chickens; growth performance; antioxidant; immune; jejunum morphology

CLC Number:

S831.5

SONG Qiongli, CHEN Jiang, WU Dong, ZOU Zhiheng, GONG Jianping, CHEN Xiaolian, SONG Wenjing, XIONG Pingwen, XU Chuanhui, LI Mengchu, SU Weide, AI Gaoxiang. Effects of Tetrastigma hemsleyanum Leaves Powder on Growth Performance,Serum Biochemical Index and Jejunum Morphology of Chongren Patridge Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2481-2488.

References

[1] 郭盛,段金廒,赵明,等.基于药材生产与深加工过程非药用部位及副产物开发替代抗生素饲用产品的可行性分析与研究实践[J].中草药,2020,51(11):2857-2862. GUO S,DUAN J A,ZHAO M,et al.Feasibility analysis and research practice for development of alternative antibiotic feeding products based on non-medicinal parts and by-products in processing of Chinese medicinal materials[J].Chinese Traditional and Herbal Drugs,2020,51(11):2857-2862.(in Chinese)
[2] ABEDIN A,PEI Z,QINGZHEN Z,et al.Application of traditional Chinese herbal medicine by-products as dietary feed supplements and antibiotic replacements in animal production[J].Current Drug Metabolism,2019,20(1):54-64
[3] 蔡韦炜,陈丹,范世明,等.中药三叶青化学成分及药理作用研究进展[J].天津药学,2014,26(1):38-41. CAI W W,CHEN D,FAN S M,et al.Research review on chemical components and pharmacological effects of Tetrastigmatis hemsleyanum[J].Tianjin Pharmacy,2014,26(1):38-41.(in Chinese)
[4] 廖淑彬,陈丹,余文静,等.三叶青地上部分抗炎提取物效应组分群不同生理状态体内组织分布规律及特性研究[J].中国中药杂志,2021,46(5):1224-1249. LIAO S B,CHEN D,YU W J,et al.Study on patterns and characteristics of in vivo tissue distribution of anti-inflammatory extract of Tetrastigma hemsleyanum aerial part in healthy and inflammatory pathological model rats[J].China Journal of Chinese Materia Medica,2021,46(5):1224-1249.(in Chinese)
[5] 廖淑彬,蔡韦炜,陈丹,等.闽产三叶青地上部分提取物体内抗炎镇痛作用研究[J].中国现代应用药学,2017,34(3):319-324. LIAO S B,CAI W W,CHEN D,et al.Anti-inflammatory and analgesic effects of the extracts of Tetrastigmatis hemsleyanum’s aerial parts from Fujian in vivo[J].Chinese Modern Applied Pharmacy,2017,34(3):319-324.(in Chinese)
[6] 姜文娟,陈文云,徐田放,等.三叶青研究现状及其在畜牧兽医领域的前景展望[J].中兽医学杂志,2018,4:96-98. JIANG W J,CHEN W Y,XU T F,et al.Current research status and prospect of Tetrastigmatis hemsleyanum in animal husbandry and veterinary field[J].Chinese Journal of Traditional Veterinary Science,2018,4:96-98.(in Chinese)
[7] 阮明颖.不同产地三叶青总黄酮及多糖含量的测定[J].当代化工研究,2021,22:19-23. RUAN M Y.Determination of total flavonoids and polysaccharide contents in Tetrastigma hemsleyanum Diels et Gilg from different areas[J].Modern Chemical Research,2021,22:19-23.(in Chinese)
[8] 中华人民共和国农业部.鸡饲养标准:NY/T 33—2004[S].北京:中国农业出版社,2004. MINISTRY OF AGRICULTURE OF THE PRC.Feeding standard of chicken:NY/T 33—2004[S].Beijing:China Agriculture Press,2004.(in Chinese)
[9] 宋琼莉,宋文静,邹志恒,等.复合添加剂对宁都黄鸡生长性能、血清免疫和抗氧化功能以及肠道健康的影响[J].动物营养学报,2022,34(11):7120-7132. SONG Q L,SONG W J,ZOU Z H,et al.Effects of compound additives on growth performance,serum immune and antioxidant function and intestine health of Ningdu Yellow chickens[J].Chinese Journal of Animal Nutrition,2022,34(11):7120-7132.(in Chinese)
[10] JI T,JI W W,WANG J,et al.A comprehensive review on traditional uses,chemical compositions,pharmacology properties and toxicology of Tetrastigma hemsleyanum[J].Journal of Ethnopharmacology,2020,264:113247.
[11] 陈加红.药用植物三叶青藤蔓在肉牛养殖中的应用[J].智慧农业导刊,2022,2(1):21-23. CHEN J H.Application of medicinal plant Trifoliate vine in beef cattle breeding[J].Journal of Smart Agriculture,2022,2(1):21-23.(in Chinese)
[12] 王晶,刘滔,赵敏洁,等.三叶青地上部分对肉鸡肠道菌群、免疫功能和生长性能的影响[J].浙江大学学报(农业与生命科学版),2023,49(6):863-872. WANG J,LIU T,ZHAO M J,et al.Effects of aboveground parts of Tetrastigma hemsleyanum on the intestinal microbiota,immune function and growth performance of broilers[J].Journal of Zhejiang University (Agriculture & Life Sciences),2023,49(6):863-872.(in Chinese)
[13] 蔡韦炜,陈丹,范世明,等.三叶青地上部分化学成分分析[J].福建中医药大学学报,2013,23(5):34-35,42. CAI W W,CHEN D,FAN S M,et al.Component analysis of aerial parts of Tetrastigma hemsleyanum[J].Journal of Fujian University of Chinese Medicine,2013,23(5):34-35,42.(in Chinese)
[14] ZHANG Y W,LUO H L,CHANG Y F,et al.Effects of liquorice extract on the activity and gene expression level of antioxidant enzymes in longissimus dorsi muscle of Tan lamb[J].Small Ruminant Research,2017,154:23-28.
[15] 张进军,张作法,吕国英,等.浙产三叶青地下地上部分抗氧化活性比较[J].浙江农业科学,2021,62(4):709-711. ZHANG J J,ZHANG Z H,LYU G Y,et al.Comparison of antioxidant activity of the underground and the ground parts of Tetrastigma hemsleyanum from Zhejiang[J].Journal of Zhejiang Agricultural Sciences,2021,62(4):709-711.(in Chinese)
[16] 刘伟民,熊科辉,刘洋,等.三叶青叶不同萃取物的抗氧化和抗炎活性评价[J].浙江林业科技,2023,43(5):59-65. LIU W M,XIONG K H,LIU Y,et al.Experiment on antioxidant and anti-inflammatory activity of different extracts from leaves of Tetrastigma hemsleyanum[J].Zhejiang Forestry Science Technology,2023,43(5):59-65.(in Chinese)
[17] FATHI M M,EBEID T A,AL-HOMIDAN I,et al.Influence of probiotic supplementation on immune response in broilers raised under hot climate[J].British Poultry Science,2017,58(5):512-516.
[18] SHENGHE L,ERHUI J,ENMEI Q,et al.Chitooligosaccharide promotes immune organ development in broiler chickens and reduces serum lipid levels[J].Histology and Histopathology,2017,32(9):951-961.
[19] 刘梦杰,周静,李越,等.马齿苋、苦参、伏龙肝复方中草药添加剂对肉鸡生长性能、抗氧化能力、免疫功能和肠道形态及屏障功能的影响[J].动物营养学报,2023,35(6):3644-3658. LIU M J,ZHOU J,LI Y,et al.Effects of Portolaca oleracea L.,Sophorae flavescentis,terra flava usta compound Chinese herbal medicine additive on growth performance,antioxidant ability,immune function,intestinal morphology and barrier function of broilers[J].Chinese Journal of Animal Nutrition,2023,35(6):3644-3658.(in Chinese)
[20] 邢倩,郑溜丰,甘婷,等.三叶青主要成分抗炎作用及其机制[J].食品与机械,2020,36(5):46-53. XING Q,ZHENG L F,GAN T,et al.Anti-inflammatory effect and mechanism of major components in Tetrastigma hemsleyanum Diels et Gilg[J].Food & Machinery,2020,36(5):46-53.(in Chinese)
[21] JI W W,PENG X,LOU T L,et al.Total flavonoids from Tetrastigma hemsleyanum ameliorates inflammatory stress in concanavalin A-induced autoimmune hepatitis mice by regulating Treg/Th17 immune homeostasis[J].Inflammopharmacology,2019,27(6):1297-1307.
[22] 王建烽.可溶性纤维日粮对福建黄兔生产性能、免疫机能以及肠道内环境的影响[D].福州:福建农林大学,2018. WANG J F.Effect of soluble fiber diet on performance,immune function and internal cecum of Fujian rabbit[D].Fuzhou:Fujian Agriculture and Forestry University,2018.(in Chinese)
[23] ZHU X P,SHANG X G,LING Z H,et.al.Effects of zinc glycinate on growth performance,serum biochemical indexes,and intestinal morphology of Yellow feather broilers[J].Biological Trace Element Research,2022,200(9):4089-4097.
[24] AN J S,SHI J J,LIU K B,et al.Effects of solid-state fermented wheat bran on growth performance,immune function,intestinal morpholopy and microflora in lipopolysaccharide-challenged broiler chickens[J].Animals,2022,12(9):1100.
[25] AWAD W A,HESS C,HESS M.Enteric pathogens and their toxin-induced disruption of the intestinal barrier through alteration of tight junctions in chickens[J].Toxins,2017,9(2):60.

Effects of Tetrastigma hemsleyanum Leaves Powder on Growth Performance,Serum Biochemical Index and Jejunum Morphology of Chongren Patridge Chickens

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