豆蔻明对慢性热应激儋州鸡脏器系数、血清生化指标、抗氧化能力及炎性因子的影响

doi:10.16431/j.cnki.1671-7236.2024.03.016

Abstract

Abstract: 【Objective】 The study aimed to explore the effects of cardamomamine (CDN) on organ coefficients,serum biochemical indexes,antioxidant capacity and inflammatory factors in chronic heat-stressed Danzhou chickens.【Method】 200 1-day-old Danzhou hens were selected for the experiment and randomly divided into 5 groups:Control group (CON),heat stress group (HS),low-dose (L-CDN),medium-dose (M-CDN),and high-dose cardamom group (H-CDN).Each group had 4 replicates,with 10 chickens per replicate.The hens in CON and HS groups were fed a basic diet,while in L-CDN,M-CDN,and H-CDN groups were added 50,100,and 200 mg/kg cardamom to the basic diet,respectively.The experiment lasted for 42 days,with a pre-trial period of 21 days and a formal trial period of 21 days.During the formal trial period,the HS,L-CDN,M-CDN,and H-CDN groups were kept at a temperature of (36±2) ℃ from 09:00 to 16:00 each day.The rest of the time,the temperature was maintained at (25±2) ℃,while the CON group was kept at a temperature of (25±2) ℃ all day.At the end of 42 days,two Danzhou chickens with similar weights were selected from each replicate for slaughter.Serum was collected to detect biochemical indicators,antioxidant indicators,and serum inflammatory factors.The liver,spleen,thymus,and bursa of Fabricius were collected to calculate organ coefficients.【Result】 ①The levels of alanine aminotransferase (ALT) in the serum of Danzhou chickens in M-CDN and H-CDN groups were significantly lower than those in HS group (P＜0.05), while the levels of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total cholesterol (TC), and triglycerides (TG) in the serum of chickens in L-CDN, M-CDN, and H-CDN groups were significantly lower than those in HS group (P＜0.05). Except for the significantly lower TG content in H-CDN group compared to CON group (P＜0.05), there was no significant difference between H-CDN group and CON group (P＞0.05). ② Compared with HS group,the Fabricius index of Danzhou chickens in M-CDN and H-CDN groups were significant increased (P＜0.05), while the spleen index in H-CDN group was significant increased (P＜0.05). The liver index of all three experimental groups were decreased significantly (P＜0.05), and there was no significant difference between them and CON group (P＞0.05). Among them, the Fabricius index of M-CDN and H-CDN groups was significantly higher than that of L-CDN group (P＜0.05), and the spleen index of H-CDN group was significantly higher than that of L-CDN and H-CDN groups (P＜0.05). ③ Compared with HS group, the serum malondialdehyde (MDA) content of Danzhou chickens in the three experimental groups was significantly reduced (P＜0.05). The serum superoxide dismutase (SOD) activity of Danzhou chickens in M-CDN group and the serum SOD, catalase (CAT), and glutathione peroxidase (GSH-Px) activities of Danzhou chickens in H-CDN group were significantly increased (P＜0.05), and there was no significant difference compared with CON group (P＞0.05). The total antioxidant capacity (T-AOC) of the serum of Danzhou chickens in three experimental groups was significantly increased (P＜0.05), but still significantly lower than that of CON group (P＜0.05). The SOD activity in M-CDN and H-CDN groups was significantly higher than that in L-CDN group (P＜0.05), while the CAT and GSH-Px activities in H-CDN group were significantly higher than those in L-CDN and M-CDN groups (P＜0.05). ④ Compared with HS group, the serum interleukin-1β(IL-1β) content of Danzhou chickens in all three experimental groups were significantly reduced (P＜0.05). The serum IL-6 and tumor necrosis factor-α(TNF-α) contents of Danzhou chickens in M-CDN and H-CDN groups were significantly reduced, while the IL-10 levels were significantly increased (P＜0.05), and there was no significant difference compared to CON group (P＞0.05). There was no significant difference between the three experimental groups (P＞0.05).【Conclusion】 Cardamom added to feed could regulate some serum biochemical indexes of Danzhou chickens under heat stress,promote the development of immune organs to enhance immune ability,alleviate heat stress-induced liver injury,improve antioxidant ability,and alleviate heat stress-induced immune stress and inflammation.The appropriate supplement level of cardamom for chronic heat stress in Danzhou chickens was 200 mg/kg.

Key words: cardamomine; Danzhou chicken; chronic heat stress; organ coefficients; serum biochemical indexes; antioxidant capacity

CLC Number:

S831.5

DENG Tanjie, HOU Guanyu, SHI Liguang, FANG Yi, WU Xiaomei, XUN Wenjuan. Effects of Cardamomine on Organ Coefficients,Serum Biochemical Indexes, Antioxidant Capacity and Inflammatory Factors of Chronically Heat-stressed Danzhou Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1050-1059.

References

[1] ROSTAGNO MARCOS H.Effects of heat stress on the gut health of poultry[J].Journal of Animal Science, 2020, 98(4):skaa090.
[2] ROMANOVSKY A A.Thermoregulation:Some concepts have changed.Functional architecture of the thermoregulatory system[J].American Journal of Physiology.Regulatory, Integrative and Comparative Physiology, 2007, 292(1):R37-R46.
[3] 邓谭杰,荀文娟,侯冠彧,等.热应激对鸡肠黏膜屏障的影响及其营养调控研究进展[J].中国家禽, 2023, 45(5):92-99. DENG T J, XUN W J, HOU G Y, et al.Research progress on the effect of heat stress on intestinal mucosal barrier and nutrition manipulation in chickens[J].China Poultry, 2023, 45(5):92-99.(in Chinese)
[4] HATZIIEREMIA S, GRAY A I, FERRO V A, et al.The effects of cardamonin on lipopolysaccharide-induced inflammatory protein production and MAP kinase and NF-κB signalling pathways in monocytes/macrophages[J].British Journal of Pharmacology, 2006, 149(2):188-198.
[5] REN G, SUN A, DENG C, et al.The anti-inflammatory effect and potential mechanism of cardamonin in DSS-induced colitis[J].American Journal of Physiology.Gastrointestinal and Liver Physiology, 2015, 309(7):G517-G527.
[6] EWA P, BO AZG ENA K, WOJCIECH Z, et al.Effect of extruded amaranth grains on performance, egg traits, fatty acids composition, and selected blood characteristics of laying hens[J].Livestock Science, 2013, 155(2-3):308-315.
[7] 连锐锐,李桂明,孙传熙,等.HSP在热应激条件下调控鸡心肌细胞损伤的功能分析[J].动物医学进展, 2021, 42(2):33-40. LIAN R R, LI G M, SUN C X, et al.Functional analysis of HSP regulating chicken myocardial cell damage under heat stress[J]. Advances in Animal Medicine, 2021, 42(2):33-40.(in Chinese)
[8] 刘峰成,孟田田,郅可欣,等.白藜芦醇对热应激诱导肉鸡脾脏淋巴细胞增殖及凋亡的作用研究[J].动物营养学报, 2023, 35(7):4604-4616. LIU F C, MENG T T, ZHAN K X, et al.Effects of resveratrol on proliferation and apoptosis of spleen lymphocytes in broilers under heat stress[J].Journal of Animal Nutrition, 2023, 35(7):4604-4616.(in Chinese)
[9] XIN F, NONG K Y, QIN X Y, et al.Effect of purple sweet potato-derived anthocyanins on heat stress response in Wenchang chickens and preliminary mechanism study[J]. Poultry Science, 2023, 102(9):102861.
[10] HIRATA H, TAKAZUMI K, SEGAWA S, et al.Xanthohumol, a prenylated chalcone from Humulus lupulus L., inhibits cholesteryl ester transfer protein[J].Food Chemistry, 2012, 134(3):1432-1437.
[11] LU Z, HE X F, MA B, et al.Increased fat synthesis and limited apolipoprotein B cause lipid accumulation in the liver of broiler chickens exposed to chronic heat stress[J].Poultry Science, 2019, 98(9):3695-3704.
[12] GUO Y, BALASUBRAMANIAN B, ZHAO Z, et al.Heat stress alters serum lipid metabolism of Chinese indigenous broiler chickens--A lipidomics study[J].Environmental Science and Pollution Research, 2021, 28:10707-10717.
[13] WANG Y, YI L, ZHAO M L, et al.Effects of zinc-methionine on growth performance, intestinal flora and immune function in pigeon squabs[J].British Poultry Science, 2014, 55(3):403-408.
[14] 习羽,方璧君,徐晶云,等.维生素A和维生素K3对产蛋后期蛋鸡生产性能、蛋品质、肝脏指数和脂质代谢的影响[J].动物营养学报, 2021, 33(7):3863-3872. XI Y, FANG B J, XU J Y, et al. Effects of vitamin a and vitamin k;on performance, egg quality, liver index and lipid metabolism of laying hens during later laving period[J].Chinese Journal of Animal Nutrition, 2021, 33(7):3863-3872.(in Chinese)
[15] LIN H, EDDY D, JOHAN B.Acute heat stress induces oxidative stress in broiler chickens[J].Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology, 2006, 144(1):11-17.
[16] CHAUHAN S S, RASHAMOL V P, BAGATH M, et al.Impacts of heat stress on immune responses and oxidative stress in farm animals and nutritional strategies for amelioration[J].International Journal of Biometeorology, 2021, 65:1231-1244.
[17] 雷昕.EGCG对热应激肉鸡肠道结构与抗氧化性能的影响[D].荆州:长江大学, 2019. LEI X.Effects of EGCG on intestinal structure and antioxidant properties of heat-stressed broilers[D].Jingzhou:Yangtze University, 2019.(in Chinese)
[18] 邓菊婴,胡睿智,肖定福,等.白藜芦醇缓解家禽热应激作用机制与应用研究进展[J].动物营养学报, 2022, 34(5):2773-2780. DENG J Y, HU R Z, XIAO D F, et al. Research progress on mechanism and application of resveratrol in alleviating heat stress in poultry[J].Chinese Journal of Animal Nutrition, 2022, 34(5):2773-2780.(in Chinese)
[19] LI Y Y, HUANG S S, LEE M M, et al.Anti-inflammatory activities of cardamonin from Alpinia katsumadai through heme oxygenase-1 induction and inhibition of NF-κB and MAPK signaling pathway in the carrageenan-induced paw edema[J].International Immunopharmacology, 2015, 25(2):332-339.
[20] 姬孟瑶,杨文,邓谭杰,等.豆蔻明对免疫应激仔猪脏器指数、血清生化指标、炎性因子及抗氧化能力的影响[J].饲料研究, 2023, 46(6):15-19. JI M Y, YANG W, DENG T J, et al.Effect of cardamonin on organ index, serum biochemical indexes, inflammatory factor and serum antioxidant capacity in immune stressed piglets[J].Feed Research, 2023, 46(6):15-19.(in Chinese)
[21] WANG J H, XUE X H, LIU Q, et al.Effects of duration of thermal stress on growth performance, serum oxidative stress indices, the expression and localization of ABCG2 and mitochondria ROS production of skeletal muscle, small intestine and immune organs in broilers[J].Journal of Thermal Biology, 2019, 85:102420.
[22] SHERWOOD E R, TOLIVER-KINSKY T.Mechanisms of the inflammatory response[J].Best Practice&Research Clinical Anaesthesiology, 2004, 18(3):385-405.
[23] SCHMITT M, FLORIAN R G.The inflammatory pathogenesis of colorectal cancer[J].Nature Reviews Immunology, 2021, 21(10):653-667.
[24] MALEKI S J, CRESPO J F, CABANILLAS B.Anti-inflammatory effects of flavonoids[J].Food Chemistry, 2019, 299:125124.
[25] NAWAZ J, RASUL A, SHAH M, et al.Cardamonin:A new player to fight cancer via multiple cancer signaling pathways[J].Life Sciences, 2020, 250:117591.
[26] JAMES S, APARNA J S, BABU A, et al.Cardamonin attenuates experimental colitis and associated colorectal cancer[J].Biomolecules, 2021, 11(5):661.
[27] HOU S, YUAN Q, YU N, et al.Cardamonin attenuates chronic inflammation and tumorigenesis in colon[J].Cell Cycle, 2019, 18(23):3275-3287.
[28] WANG Z, XU G, GAO Y, et al.Cardamonin from a medicinal herb protects against LPS-induced septic shock by suppressing NLRP3 inflammasome[J].Acta Pharmaceutica Sinica B, 2019, 9(4):734-744.

Effects of Cardamomine on Organ Coefficients,Serum Biochemical Indexes, Antioxidant Capacity and Inflammatory Factors of Chronically Heat-stressed Danzhou Chickens

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