蒲公英和连翘提取物对隐性乳房炎奶牛生产性能、血清抗氧化及免疫指标的影响

doi:10.16431/j.cnki.1671-7236.2024.04.017

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the effects of dandelion and Forsythia extracts on production performance,serum antioxidant and immune indexes of dairy cows with subclinical mastitis,in order to provide theoretical basis for the development and application of green feed additives based on plant extract.【Method】 18 multiparous Holstein cows with similar body condition,lactation period,milk production,and the mixed milk sample with somatic cell count (SCC) in milk ＞5×10⁵/mL were selected.According to the principle of similar SCC,milk samples were divided into control group (CON group),dandelion group (DAN group) and Forsythia group (FOR group).The cows in CON group were fed a total mixed diet (TMR),the cows in DAN group were fed TMR＋dandelion extract,and the cows in FOR group were fed TMR＋Forsythia extract.None of the cows received any drug treatment within 1 week before the experiment and the experiment lasted for 45 days.Milk samples were collected every 7 days to determine milk composition and SCC.After the experiment,milk samples were collected to determine the activities of bovine myeloperoxidase (MPO),lactate dehydrogenase (LDH) and N-acetyl-β-D-glucosaminase (NAG).Blood samples were collected and serum antioxidant and immune indexs were measured.【Result】 Compared with the CON group,① Dietary supplementation of dandelion and Forsythia extract had no significant effect on milk production and milk components of dairy cows with subclinical mastitis (P＞0.05).② The LDH activity in milk of DAN and FOR groups was significantly decreased (P＜0.05).NAG activity in DAN group was significantly decreased (P＜0.05).③ The catalase (CAT) activity in the serum of dairy cows with subclinical mastitis in DAN group was significantly increased (P＜0.05),and the total antioxidant capacity (T-AOC) in FOR group was significantly increased (P＜0.05).Both herbal extracts could significantly reduce the serum malondialdehyde (MDA) content of dairy cows with subclinical mastitis (P＜0.05).④ The contents of IgA,IgG and IgM in serum of dairy cows with subclinical mastitis in DAN and FOR groups were significantly increased (P＜0.05),and the IL-1β content in serum was significantly reduced (P＜0.05).【Conclusion】 Dietary supplementation of dandelion and Forsythia extracts could regulate the inflammatory response,enhance the immune and antioxidant capacity of dairy cows with subclinical mastitis,and thus achieve the prevention and control effect of subclinical mastitis in dairy cows.

Key words: dairy cows; subclinical mastitis; dandelion; Forsythia; production performance; antioxidant

CLC Number:

S816.7

ZHANG Tenglong, GUO Chenyang, ZHONG Huachen, LIU Jialin, SONG Jie, WANG Lifang. Effects of Dandelion and Forsythia Extracts on Production Performance,Serum Antioxidant and Immune Indexes of Dairy Cows With Subclinical Mastitis[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1490-1499.

References

[1] CASTELANI L,ARCARO J R P,BRAGA J E P,et al.Short communication:Activity of nisin,lipid bilayer fragments and cationic nisin-lipid nanoparticles against multidrug-resistant Staphylococcus spp.isolated from bovine mastitis[J].Journal of Dairy Science,2019,102(1):678-683.
[2] BLOWEY R W,BLOWEY R W,EDMONDSON P.Mastitis control in dairy herds[M].2nd ed.Ipswich:Farming Press Books,2010.
[3] SADEK K,SALEH E,AYOUB M.Selective,reliable blood and milk bio-markers for diagnosing clinical and subclinical bovine mastitis[J].Tropical Animal Health and Production,2017,49(2):431-437.
[4] CHEN X,CHEN Y,ZHANG W,et al.Prevalence of subclinical mastitis among dairy cattle and associated risks factors in China during 2012-2021:A systematic review and Meta-analysis[J].Research in Veterinary Science,2022,148:65-73.
[5] WHITE D G,MCDERMOTT P F.Emergence and transfer of antibacterial resistance[J].Journal of Dairy Science,2001,84:E151-E155.
[6] LHERMIE G,GRÖHN Y T,RABOISSON D.Addressing antimicrobial resistance:An overview of priority actions to prevent suboptimal antimicrobial use in food-animal production[J].Frontiers in Microbiology,2016,7:2114.
[7] 甘利平,杨维仁,张崇玉,等.植物提取物的生物学功能及其作用机理[J].动物营养学报,2015,27(9):2667-2675. GAN L P,YANG W R,ZHANG C Y,et al.Biological functions of plant extracts and its mechanisms[J].Chinese Journal of Animal Nutrition,2015,27(9):2667-2675.(in Chinese)
[8] LIU Y,ESPINOSA C D,ABELILLA J J,et al.Non-antibiotic feed additives in diets for pigs:A review[J].Animal Nutrition,2018,4(2):113-125.
[9] OH N S,LEE J Y,JOUNG J Y,et al.Microbiological characterization and functionality of set-type yogurt fermented with potential prebiotic substrates Cudrania tricuspidata and Morus alba L.leaf extracts[J].Journal of Dairy Science,2016,99(8):6014-6025.
[10] FEKRAZAD R,AFZALI M,PASBAN-ALIABADI H,et al.Cytotoxic effect of Thymus caramanicus Jalas on human oral epidermoid carcinoma KB cells[J].Brazilian Dental Journal,2017,28(1):72-77.
[11] KHER M N,SHETH N R,BHATT V D.In vitro antibacterial evaluation of Terminalia chebula as an alternative of antibiotics against bovine subclinical mastitis[J]. Animal Biotechnology,2019,30(2):151-158.
[12] MONTIRONI I D,CARIDDI L N,REINOSO E B.Evaluation of the antimicrobial efficacy of Minthostachys verticillata essential oil and limonene against Streptococcus uberis strains isolated from bovine mastitis[J].Revista Argentina de Microbiología,2016,48(3):210-216.
[13] SAMOLIŃSKA W,GRELA E R,KOWALCZUK-VASILEV E,et al.Evaluation of garlic and dandelion supplementation on the growth performance,carcass traits,and fatty acid composition of growing-finishing pigs[J].Animal Feed Science and Technology,2020,259:114316.
[14] QURESHI S,BANDAY M T,ADIL S,et al.Effect of dandelion leaves and fenugreek seeds with or without enzyme addition on performance and blood biochemistry of broiler chicken,and evaluation of their in vitro antibacterial activity[J].Indian Journal of Animal Sciences,2015,85(11):1248-1254.
[15] WANG L,PIAO X L,KIM S W,et al.Effects of Forsythia suspensa extract on growth performance,nutrient digestibility,and antioxidant activities in broiler chickens under high ambient temperature[J].Poultry Science,2008,87(7):1287-1294.
[16] LU T,PIAO X L,ZHANG Q,et al.Protective effects of Forsythia suspensa extract against oxidative stress induced by diquat in rats[J].Food and Chemical Toxicology,2010,48(2):764-770.
[17] HAN X,PIAO X S,ZHANG H Y,et al.Forsythia suspensa extract has the potential to substitute antibiotic in broiler chicken[J].Asian-Australasian Journal of Animal Sciences,2012,25(4):569-576.
[18] ZHAO P,PIAO X,ZENG Z,et al.Effect of Forsythia suspensa extract and chito-oligosaccharide alone or in combination on performance,intestinal barrier function,antioxidant capacity and immune characteristics of weaned piglets[J].Animal Science Journal,2017,88(6):854-862.
[19] 钟华晨,张三粉,冯小慧,等.植物提取物对奶牛乳房炎致病菌的抑菌效果研究[J].黑龙江畜牧兽医,2020,23:117-121. ZHONG H C,ZHANG S F,FENG X H,et al.Study on the antibacterial effect of plant extracts on the pathogenic bacteria of dairy cow mastitis[J].Heilongjiang Animal Science and Veterinary Medicine,2020,23:117-121.(in Chinese)
[20] 郭晨阳,张腾龙,徐腾腾,等.复合植物提取物对荷斯坦奶牛体外瘤胃发酵的影响[J].动物营养学报,2020,32(8):3698-3707. GUO C Y,ZHANG T L,XU T T,et al.Effects of compound plant extracts on rumen fermentation of Holstein cows in vitro[J].Chinese Journal of Animal Nutrition,2020,32(8):3698-3707.(in Chinese)
[21] OLAGARAY K E,BROUK M J,MAMEDOVA L K,et al.Dietary supplementation of Scutellaria baicalensis extract during early lactation decreases milk somatic cells and increases whole lactation milk yield in dairy cattle[J].PLoS One,2019,14(1):e0210744.
[22] KHURANA R,BRAND T,TAPIO I,et al.Effect of a garlic and citrus extract supplement on performance,rumen fermentation,methane production,and rumen microbiome of dairy cows[J].Journal of Dairy Science,2023,106(7):4608-4621.
[23] JIA P,TU Y,LIU Z H,et al.Diets supplementation with Bacillus subtilis and Macleaya cordata extract improve production performance and the metabolism of energy and nitrogen,while reduce enteric methane emissions in dairy cows[J].Animal Feed Science and Technology,2022,294:115481.
[24] SCHUKKEN Y H,WILSON D J,WELCOME F,et al.Monitoring udder health and milk quality using somatic cell counts[J].Veterinary Research,2003,34(5):579-596.
[25] 郝景锋,李静姬,尹柏双,等.中药复方对奶牛隐性乳房炎血清中LDH、NAG活性的影响[J].黑龙江畜牧兽医,2017,24:134-136. HAO J F,LI J J,YIN B S,et al.Effects of Chinese herbal compound on the activities of LDH and NAG in serum of subclinical mastitis cows[J].Heilongjiang Animal Science and Veterinary Medicine,2017,24:134-136.(in Chinese)
[26] 张永英,钟翠红,王慧真,等.乳房炎患牛血清生化指标的观察[J].黑龙江畜牧兽医,2014,20:53-54. ZHANG Y Y,ZHONG C H,WANG H Z,et al.The effect of mastitis on the serum biochemical indexes in dairy cattle[J].Heilongjiang Animal Science and Veterinary Medicine,2014,20:53-54.(in Chinese)
[27] LEITNER G,CHAFFER M,CARASO Y,et al.Udder infection and milk somatic cell count,NAGase activity and milk composition—Fat,protein and lactose—In Israeli-Assaf and Awassi sheep[J].Small Ruminant Research,2003,2(49):157-164.
[28] 王红连.防治奶牛乳房炎中药方剂的研究[D].太原:山西农业大学,2015. WANG H L.Study of TCM prescriptions on the prophylaxis and therapy of dairy cow mastitis[D].Taiyuan:Shanxi Agricultural University,2015.(in Chinese)
[29] 杨德英.奶牛乳房炎对乳酶活性、急性期蛋白含量的影响及其作为隐性乳房炎诊断指标的研究[D].成都:四川农业大学,2009. YANG D Y.Effects of bovine mastitis on activities of milk enzymes,contents of acute phase proteins and as potential indicator of cows’ subclinical mastitis[D].Chengdu:Sichuan Agricultural University,2009.(in Chinese)
[30] DEPREESTER E,MEYER E,DEMEYERE K,et al.Flow cytometric assessment of myeloperoxidase in bovine blood neutrophils and monocytes[J].Journal of Dairy Science,2017,100(9):7638-7647.
[31] 郭昌明,张乃生,周昌芳,等.髓过氧化物酶检测奶牛隐性乳房炎研究进展[J].动物医学进展,2006,6:54-57. GUO C M,ZHANG N S,ZHOU C F,et al.Development of the myeloperoxidase detecting bovine latent mastitis[J].Progress in Veterinary Medicine,2006,6:54-57.(in Chinese)
[32] 李光辉,任永红.氧化应激对奶牛健康的危害及其防治措施的研究进展[J].畜牧兽医杂志,2021,40(1):35-37. LI G H,REN Y H.Research progress of oxidative stress on dairy cow health and its prevention and treatment measures[J].Journal of Animal Science and Veterinary Medicine,2021,40(1):35-37.(in Chinese)
[33] 聂宇泽,吴汉东,张玉科.黄芪、蒲公英和金银花复方中草药对奶牛产奶性能及血清生化指标的影响[J].饲料研究,2023,9:10-14. NIE Y Z,WU H D,ZHANG Y K.Effect of Astragalus,dandelion and honeysuckle compound Chinese herbal medicine on milk production performance and serum biochemical indices of dairy cows[J].Feed Research,2023,9:10-14.(in Chinese)
[34] 孙雅望.脂多糖引起奶牛乳腺和机体氧化应激损伤及蒲公英提取物对其阻断效果的研究[D].重庆:西南大学,2021. SUN Y W.A study on the mammary and systematic oxidative stress induced by lipopolysaccharide in bovine and the blocking effect of dandelion extract[D].Chongqing:Southwest University,2021.(in Chinese)
[35] LONG S F,HE T F,WU D,et al.Forsythia suspensa extract enhances performance via the improvement of nutrient digestibility,antioxidant status,anti-inflammatory function,and gut morphology in broilers[J].Poultry Science,2020,99(9):4217-4226.
[36] 高雅,孙满吉,孙东峰,等.蒲公英对放牧肉牛血清代谢产物及增重的影响[J].中国饲料,2013,16:20-22. GAO Y,SUN M J,SUN D F,et al.Effect of dandelion on serum metabolites and weight gain of grazing beef cattle[J].China Feed,2013,16:20-22.(in Chinese)
[37] LONG S F,WU D,HE T F,et al.Dietary supplementation with Forsythia suspensa extract during late gestation improves reproductive performance,colostrum composition,antioxidant status,immunoglobulin,and inflammatory cytokines in sows and newborn piglets[J].Animal Feed Science and Technology,2021,271:114700.
[38] LEAL A E B P,DE OLIVEIRA A P,SANTOS R F D,et al.Determination of phenolic compounds,in vitro antioxidant activity and characterization of secondary metabolites in different parts of Passiflora cincinnata by HPLC-DAD-MS/MS analysis[J].Natural Product Research,2020,34(7):995-1001.
[39] 侯昆,童津津,楚康康,等.竹叶黄酮与青蒿提取物对患隐性乳房炎奶牛产奶性能、乳中体细胞数及血清免疫和抗氧化相关指标的影响[J].动物营养学报,2019,31(9):4286-4295. HOU K,TONG J J,CHU K K,et al.Effects of bamboo leaf flavonoids and Artemisia annua extract on milk performance,milk somatic cell count and serum immune and antioxidant related indexes of dairy cows with subclinical mastitis[J].Chinese Journal of Animal Nutrition,2019,31(9):4286-4295.(in Chinese)
[40] 占今舜,苏效双,刘明美,等.日粮中添加苜蓿黄酮对奶牛血液生化指标、抗氧化性能和免疫的影响[J].中国农业大学学报,2017,22(5):66-74. ZHAN J S,SU X S,LIU M M,et al.Effects of diet added alfalfa flavonoids on blood biochemical indexes,oxidation resistance and immunity of dairy cow[J].Journal of China Agricultural University,2017,22(5):66-74.(in Chinese)

Effects of Dandelion and Forsythia Extracts on Production Performance,Serum Antioxidant and Immune Indexes of Dairy Cows With Subclinical Mastitis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MA Xiaoxue, LONG Rui, NIU Yujie, GUO Hongyong, LUO Ruiqing, WU Yanyan, ZHANG Wenju. Effect of Adding Isoacids in Perinatal Diet on Rumen Fermentation Parameters, Rumen Microorganisms and Production Performance of Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1428-1437.
[2]	LUO Chunhai, ZHENG Chengyuan, ZHANG Menglong, YAO Weijia, LIU Jiajin, LIU Bingqi, WANG Wei, FU Shixin. Regulation of Vascular Endothelial Growth Factor A Expression by miRNA-185 in Dairy Cows with Retained Fetal Membranes [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 916-925.
[3]	LI Chunxin, QIAO Hongxing, WANG Huiwei, FAN Mingxia, WANG Jiwen. Effects of Compound Fermentation of Traditional Chinese Medicine and Cyperus esculentus on Growth Performance,Serum Antioxidant and Immune Indexes in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 990-998.
[4]	CHENG Wenhao, XU Yu, SONG Kelin, SHI Haixia, YAN Zunqiang, WANG Pengfei, GUN Shuangbao. Effects of Whole Plant Silage Maize Meal on Growth Performance,Serum Biochemical Indicators,Antioxidant Capacity and Immunological Indicators of Fattening Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1021-1030.
[5]	DENG Tanjie, HOU Guanyu, SHI Liguang, FANG Yi, WU Xiaomei, XUN Wenjuan. Effects of Cardamomine on Organ Coefficients,Serum Biochemical Indexes, Antioxidant Capacity and Inflammatory Factors of Chronically Heat-stressed Danzhou Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1050-1059.
[6]	WU Qingyao, YIN Yunju, WANG Min, PAN Junyi, LI Fengna, ZHAO Shengguo, CHEN Guoshun, GUO Qiuping. Effects of Chlorogenic Acid and Leucine on the Carcass Traits,Meat Quality and Serum Biochemical Indexes of Fattening Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1060-1068.
[7]	ZHOU Yingqing, LIU Yuxuan, YE Zhiwang, PEI Xinru, DU Xin, GAO Yang. Development of Squalene and Its Application in Animal Production [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1077-1085.
[8]	ZHANG Wenxiang, WANG Jianping. Effect of Catalase on the Production Performance,Antioxidant Capacity, Cecal Microbe and Metabolites in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1086-1093.
[9]	CHAN Yanzi, WANG Xinyue, LI Sihan, WANG Junkai, WANG Yuyan, HU Tiesheng, MO Guifen, LIANG Dehong, SHI Dayou. Effects of Compound Traditional Chinese Medicine on Production Performance and Reproductive Function of Forced Molting Laying Hens [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1151-1159.
[10]	LIU Yingkun, GUO Wei, FU Zengyu, SUN Meng, LI Haojia, ZHOU Haizhu. Effects of Ferulic Acid on Growth Performance and Intestinal Anti-oxidant Indicators of Jilin White Geese Under Lipopolysaccharide Stress [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 521-529.
[11]	HUANG Jing, ZHAO Na, GUO Wanzheng, JIN Feng, CHEN Fang, ZHU Wei, FAN Qiwen, DU Encun, TAO Wenjing, HUANG Shaowen, WEI Jintao. Effects of Feed Mulberry on Production Performance,Egg Quality and Intestinal Tissue Morphology of Laying Hens [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 540-548.
[12]	WANG Hechuan, LI Tianfeng, ZHANG Han, SONG Yulun, FENG Heze, WEI Yao, CHENG Ye, LI Yongxin, DONG Yingying, YIN Guoan, GUO Qing, CUI Weiguo, LI Jingchun. Effect of Feeding Zinc Lysine on Plasma Biochemical Indices and Sperm Quality of Hu Sheep Rams in Autumn in Northeastern China [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 558-567.
[13]	SUN Pengfei, WANG Xiaohan, HU Yun, WU Huiguang, ZHAO Jingwen, LUO Xugang. Research Advances on the Effects of Heat Stress on Production Performance, Serum Biochemistry and Gut Microbes in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 581-590.
[14]	ZHANG Jiaqi, YANG Kaiqi, ZHANG Jingyi, WANG Xiaoxu, WANG Ye, WANG Rui, YU Hanlin, CHEN Yanqing, BAO Jun, ZHANG Runxiang. Effects of Keel Bone Fracture on Fear Behavior, Production Performance and Egg Quality of Laying Hens in Later Laying Period [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 86-95.
[15]	NAN Bingyu, LI Haodong, ZANG Changjiang, LI Xiaobin, LI Fengming, LIU Tuo, LI Yunmeng, QIU Yaqi, YANG Kailun, WANG Jiaqi. Effects of Niacinamide on Production Performance and Nutrient Apparent Digestibility of Lactating Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 96-105.