过氧化氢酶对肉鸡生产性能、抗氧化能力、盲肠微生物及代谢物的影响

doi:10.16431/j.cnki.1671-7236.2024.03.020

Abstract

Abstract: 【Objective】 The effect of catalase (CAT) on the production performance,antioxidant capacity,cecal microbe and metabolites in broilers was investigated in this study,for providing theoretical foundation in the application of CAT in animal production.【Method】 600 1-day-old Yellow Feathered broiler chickens were selected and randomly divided into 5 groups:Blank control group (CON),antibiotic group (Anti),and low,medium and high-dose CAT groups (CAT1,CAT2 and CAT3),with 6 replicates in each group and 20 chickens in each replicate.Among them,CON group was fed with basic feed,Anti group added 50 mg/kg chloramphenicol to the basic diet,and CAT1,CAT2 and CAT3 groups were supplemented with 100,150 and 200 U/kg CAT in the basic diet,respectively.The feeding trial lasted for 42 d.After the experiment,the production performance was measured,and serum and cecal chyme were collected for measuring antioxidant indicators,microbial communities and metabolite content.【Result】 Compared with CON group,the ADG and ADFI of each dose group of CAT were significantly increased (P＜0.05),reaching the level of the Anti group.However,increasing the CAT dose did not further improve these indicators.Compared with CON group,the serum CAT and superoxide dismutase activities were significantly increased in all dose groups of CAT and Anti group (P<0.05).The levels of malondialdehyde,protein carbonyl and 8-hydroxydeoxyguanosine were significantly reduced (P＜0.05),and the 8-hydroxydeoxyguanosine levels in all dose groups of CAT were consistent with those in Anti group.Cecal microbiota analysis showed that compared with CON group,the number of Proteobacteria,Lactobacillus and Faecalibacterium in each dose group of CAT significantly increased (P<0.05),but the number of Clostridium decreased significantly (P＜0.05).Compared with CON group,the levels of lactic acid,acetic acid and isovaleric acid in the cecal contents of each dose group of CAT were significantly increased (P<0.05),while the content of valeric acid was significantly reduced (P＜0.05);The levels of methylamine,tryptamine,putrescine,cadaverine,and total amine in each dose group of CAT were significantly reduced (P＜0.05);The levels of spermidine were significantly reduced in CAT2 and CAT3 groups (P<0.05),and a comparative analysis of CAT dose effects showed that the levels of putrescine,cadaverine and total amine in CAT2 and CAT3 groups were significantly lower than those in CAT1 group (P＜0.05).【Conclusion】 Adding 150 or 200 U/kg CAT to feed could promote animal growth and health.

Key words: broiler; catalase; production performance; antioxidant capacity; cecal microbe

CLC Number:

S829.1

ZHANG Wenxiang, WANG Jianping. Effect of Catalase on the Production Performance,Antioxidant Capacity, Cecal Microbe and Metabolites in Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1086-1093.

References

[1] 刘琳,胡泽琼,乔嘉宁,等.家禽氧化还原平衡和炎症反应的营养调控研究进展[J].中国畜牧杂志, 2023, 59(1):1-9. LIU L, HU Z Q, QIAO J N, et al.Research progress on nutritional regulation of redox balance and inflammatory response in poultry[J].Chinese Journal of Animal Science, 2023, 59(1):1-9.(in Chinese)
[2] ALKADI H.A review on free radicals and antioxidants[J].Infectious Disorders-Drug Targets, 2020, 20(1):16-26.
[3] 乔凤云,陈欣,余柳青.抗氧化因子与天然抗氧化剂研究综述[J].科技通报, 2006, 22(3):332-336. QIAO F Y, CHEN X, YU L Q.A survery:Research on the antioxidative factors and natural antioxidants[J].Bulletin of Science and Technology, 2006, 22(3):332-336.(in Chinese)
[4] NEHA K, HAIDER M R, PATHAK A, et al.Medicinal prospects of antioxidants:A review[J].European Journal of Medicinal Chemistry, 2019, 178:687-704.
[5] GLORIEUX C, CALDERON P B.Catalase, a remarkable enzyme:Targeting the oldest antioxidant enzyme to find a new cancer treatment approach[J].Biological Chemistry, 2017, 398(10):1095-1108.
[6] SEPASI TEHRANI H, MOOSAVI-MOVAHEDI A A.Catalase and its mysteries[J].Progress in Biophysics and Molecular Biology, 2018, 140:5-12.
[7] 刘欣,刘云,李虎,等.过氧化氢酶对生长前期乌苏里貉生长性能、消化代谢、血清生化和免疫指标的影响[J].饲料工业, 2022, 43(13):53-58. LIU X, LIU Y, LI H, et al.Effects of catalase on growth performance, digestion and metabolism, serum biochemical and immune indices of raccoon dogs in early growth stage[J].Feed Industry, 2022, 43(13):53-58.(in Chinese)
[8] 唐明红.过氧化氢酶对黄羽肉鸡生长性能、肠道形态以及抗氧化能力的影响[J].动物营养学报, 2021, 33(7):4153-4161. TANG M H.Effects of catalase on growth performance, intestinal morphology and antioxidant capacity in Yellow broilers[J].Chinese Journal of Animal Nutrition, 2021, 33(7):4153-4161.(in Chinese)
[9] 陈嘉铭,张志东,战晓燕,等.母猪饲粮中添加过氧化氢酶对母猪及仔猪生长性能和抗氧化能力的影响[J].动物营养学报, 2019, 31(7):3268-3275. CHEN J M, ZHANG Z D, ZHAN X Y, et al.Effects of catalase supplementation in diets of sows on growth performance and antioxidant capacity of sows and piglets[J].Chinese Journal of Animal Nutrition, 2019, 31(7):3268-3275.(in Chinese)
[10] 方锐,左建军,凌宝明,等.日粮中添加过氧化氢酶对断奶仔猪生长性能、肠道形态及抗氧化性能的影响[J].中国饲料, 2017, 1:23-27. FANG R, ZUO J J, LING B M, et al.Effects of dietary addition of catalase on growth performance, intestinal morphology and antioxidant capacity of weaned piglets[J].China Feed, 2017, 1:23-27.(in Chinese)
[11] WANG J, SINGH A K, KONG F, et al.Effect of almond hulls as an alternative ingredient on broiler performance, nutrient digestibility, and cecal microbiota diversity[J].Poultry Science, 2021, 100(3):100853.
[12] LI Y, ZHAO X, JIANG X, et al.Effects of dietary supplementation with exogenous catalase on growth performance, oxidative stress, and hepatic apoptosis in weaned piglets challenged with lipopolysaccharide[J].Journal of Animal Science, 2020, 98(3):skaa067.
[13] 周天天.母猪日粮中添加过氧化氢酶对哺乳仔猪生长性能、抗氧化功能和肠道屏障的影响[D].北京:中国科学院大学, 2022. ZHOU T T.Effects of catalase addition to sow diets on growth performance, antioxidant function and intestinal barrier of nursing piglets[D].Beijing:University of the Chinese Academy of Sciences, 2022.(in Chinese)
[14] 丁嘉怡,吴涛,陈佳琪,等.基于肠道微生物探讨蛋鸡脂肪肝出血综合征的研究进展[J].中国畜牧兽医, 2023, 50(7):2888-2895. DING J Y, WU T, CHEN J Q, et al.Review on research of fatty liver hemorrhagic syndrome based on gut microbiota[J].China Animal Husbandry&Veterinary Medicine, 2023, 50(7):2888-2895.(in Chinese)
[15] 邱菲,韩依辛,魏琦麟,等.培养组学在动物肠道微生物应用的研究进展[J].中国畜牧兽医, 2023, 50(2):598-605. QIU F, HAN Y X, WEI Q L, et al.Research progress on culturomics application in animal gut microbes[J].China Animal Husbandry&Veterinary Medicine, 2023, 50(2):598-605.(in Chinese)
[16] KHAN S, MOORE R J, STANLEY D, et al.The gut microbiota of laying hens and its manipulation with prebiotics and probiotics to enhance gut health and food safety[J].Applied and Environmental Microbiology, 2020, 86(13):e00600-20.
[17] NEVELING D P, DICKS L M T.Probiotics:An antibiotic replacement strategy for healthy broilers and productive rearing[J].Probiotics and Antimicrobial Proteins, 2021, 13(1):1-11.
[18] MARTÍN R, RIOS-COVIAN D, HUILLET E, et al.Faecalibacterium:A bacterial genus with promising human health applications[J].FEMS Microbiology Reviews, 2023, 47(4):fuad039.
[19] 张金武,魏紫,冀禹彤,等.A型产气荚膜梭菌诱导黄羽肉鸡肠道氧化应激和炎性损伤研究[J].中国畜牧兽医, 2023, 50(5):2069-2081. ZHANG J W, WEI Z, JI Y T, et al.Study on oxidative stress and inflammatory injury in intestinal tract of Yellow-feathered chickens induced by Clostridium perfringens type A[J].China Animal Husbandry&Veterinary Medicine, 2023, 50(5):2069-2081.(in Chinese)
[20] WANG W, ZHU J, CAO Q, et al.Dietary catalase supplementation alleviates deoxynivalenol-induced oxidative stress and gut microbiota dysbiosis in broiler chickens[J].Toxins(Basel), 2022, 14(12):830.
[21] ZHOU J, LI M, CHEN Q, et al.Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery[J].Nature Communications, 2022, 13(1):3432.
[22] 杨瑰桃,马继登,李学伟,等.短链脂肪酸调控骨骼肌生理功能研究进展[J].中国畜牧兽医, 2023, 50(6):2286-2295. YANG G T, MA J D, LI X W, et al.Research advance on regulation of skeletal muscle physiology function by short chain fatty acid[J].China Animal Husbandry&Veterinary Medicine, 2023, 50(6):2286-2295.(in Chinese)
[23] ALI Q, MA S, LA S, et al.Microbial short-chain fatty acids:A bridge between dietary fibers and poultry gut health-A review[J].Animal Bioscience, 2022, 35(10):1461-1478.
[24] DALILE B, VAN OUDENHOVE L, VERVLIET B, et al.The role of short-chain fatty acids in microbiota-gut-brain communication[J].Nature Reviews Gastroenterology and Hepatology, 2019, 16(8):461-478.
[25] WÓJCIK W,ȽUKASIEWICZ M, PUPPEL K.Biogenic amines:Formation, action and toxicity-A review[J].Journal of the Science of Food and Agriculture, 2021, 101(7):2634-2640.

Effect of Catalase on the Production Performance,Antioxidant Capacity, Cecal Microbe and Metabolites in Broilers

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