葡萄渣对肉母牛外周血淋巴细胞亚群、淋巴细胞转化率与凋亡率及抗氧化性的影响

doi:10.16431/j.cnki.1671-7236.2024.04.021

Abstract

Abstract: 【Objective】 The aim of this study was to study the effects of grape pomace on lymphocyte subsets,lymphocyte conversion rate,apoptosis rate and antioxidant activity in peripheral blood of cows.【Method】 Twenty healthy postpartum beef cows with similar parity and body weight were randomly divided into 4 groups with 5 cows in each group.The beef cows in control group were fed a basal diet,and 240,300 and 360 g/d grape dregs were added to the basal diet per head in trial groups I,Ⅱ and Ⅲ,respectively.The pre-trial period lasted for 15 days,and the experimental period lasted for 40 days.The tail vein blood was collected and the lymphocyte subsets,the conversion rate and apoptosis rate of lymphocytes and the antioxidant level of peripheral blood were measured.【Result】 ①On the 20th day of the experiment,the proportion of CD4⁺T cells in peripheral blood of beef cows in all three experimental groups were significantly increased compared to the control group (P＜0.05).On the 40th day of the experiment,the proportion of CD4⁺T cells in groups Ⅱ and Ⅲ were significantly increased compared to the control group (P＜0.05),while the proportion of CD8⁺T cells were significantly reduced compared to the control group (P＜0.05).The CD4⁺/CD8⁺ ratio in all three experimental groups were significantly increased compared to the control group (P＜0.05).②On the 20th and 40th day of the experiment,the peripheral blood lymphocyte transformation rates of cows in groups Ⅱ and Ⅲ were significantly higher than those in the control group (P＜0.01).On the 40th day of the experiment,as the amount of grape residue added increased,the apoptosis rate of peripheral blood lymphocytes showed a linear and extremely significant decrease (P＜0.01).③On the 40th day of the experiment,the serum MDA content of three experimental groups of beef cows were significantly reduced compared to the control group (P＜0.05).The serum T-AOC content of beef cows in experimental groups Ⅰ and Ⅲ were significantly higher than that of the control group (P＜0.01).【Conclusion】 Adding different proportions of grape pomace to the diet of beef cows could improve their immune function.Under the experimental conditions,the appropriate level of grape pomace addition was (300-360) g/d.

Key words: beef cow; grape pomaces; lymphocyte; antioxidant activity

CLC Number:

S823.9

WANG Jiandong, DING Hui, LIU Xin, HOU Pengxia, LIANG Xiaojun, GAO Haihui, WANG Ruigang, JIANG Wu. Effects of Grape Pomace on Lymphocyte Subsets,Lymphocyte Transformation Rate,Apoptosis Rate and Antioxidant Activity in Peripheral Blood of Beef Cows[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1530-1536.

References

[1] TORRES J L,VARELA B,GARCIA M T,et al.Valorization of grape (Vitis vinifera) byproducts.Antioxidant and biological properties of polyphenolic fractions differing in procyanidin composition and flavonol content[J].Journal of Agricultural and Food Chemistry,2002,50(26):7548-7555.
[2] 刘芸,仇农学,杨玺玉.葡萄皮渣提取物总酚含量及体外抗氧化活性、抑菌活性[J].食品科学,2011,32(1):5-9. LIU Y,QIU N X,YANG X Y.Assessment of total phenolic content and in vitro antioxidant and antimicrobial activities of ethanol extract from grape residue left after making wine[J].Food Science,2011,32(1):5-9.(in Chinese)
[3] SOCHOROVA L,PRUSOVA B,JURIKOVA T,et al.The study of antioxidant components in grape seeds[J]. Molecules,2020,25(16):3736.
[4] 薛瑞鑫,王丹,李佳橙,等.不同比例葡萄籽饲粮对生长猪生长性能、血清生化指标、抗氧化性能和粪便微生物组成的影响[J].动物营养学报,2023,35(10):6327-6339. XUE R X,WANG D,LI J C,et al.Effects of different proportions of grape seed diets on growth performance,serum biochemical indices,antioxidant performance and fecal microbial composition of growing pigs[J].Chinese Journal of Animal Nutrition,2023,35(10):6327-6339.(in Chinese)
[5] 田跃,董红刚,沈泽锋.葡萄籽原花青素对肉鸡生长性能、免疫功能和经济效益的影响[J].饲料研究,2022,45(12):28-31. TIAN Y,DONG H G,SHENG Z F.Effect of grape seed proanthocyanidins on growth performance,immune function and economic benefit of broilers[J].Feed Research,2022,45(12):28-31.(in Chinese)
[6] ZHANG R Y,LIU J H,JIANG L S,et al.The remodeling effects of high-concentrate diets on microbial composition and function in the hindgut of dairy cows[J]. Frontiers in Nutrition,2021,8:1252.
[7] 杨德莲,童津津,张婕,等.葡萄籽原花青素对奶牛瘤胃体外发酵参数及微生物区系的影响[J].动物营养学报,2018,30(2):717-725. YANG D L,TONG J J,ZHANG J,et al.Effects of grape seed procyanidine on rumen fermentation parameters and microflora of dairy cows in vitro[J].Chinese Journal of Animal Nutrition,2018,30(2):717-725.(in Chinese)
[8] 吴永霞.不同日粮模式对奶山羊和奶牛血中内毒素含量及免疫活化状态的影响[D].重庆:西南大学,2012. WU Y X.Effect of different types of diet on plasma endotoxin and immune activation in dairy goats and dairy cows[D].Chongqing:Southwest University,2012.(in Chinese)
[9] 牟春堂,杨文军,王鹏举,等.葡萄籽原花青素对高精料育肥羔羊免疫功能的影响[J].动物营养学报,2021,33(1):330-340. MOU C T,YANG W J,WANG P J,et al.Effects of grape seed proanthocyanidins on immune function of high-concentrate fattening lambs[J]. Chinese Journal of Animal Nutrition,2021,33(1):330-340.(in Chinese)
[10] 温佳楠,周晓琪,周雨萌,等.肠道微环境与CD4⁺ T细胞及相关疾病的研究[J].现代畜牧兽医,2022,9:78-81. WEN J N,ZHOU X Q,ZHOU Y M,et al.Ntestinal microenvironment and CD4⁺T cell subsets[J].Modern Journal of Animal Husbandry and Veterinary Medicine,2022,9:78-81.(in Chinese)
[11] YANG Z,JIANG C Q,LU J Y,et al.Research progress of proanthocyanidins and anthocyanidins[J].Phytotherapy Research,2023,37(6):2552-2577
[12] 杨红洋,朱晓庆,谷新利.纯化后中药多糖对鸡新城疫疫苗免疫、T淋巴细胞转化率及IgG、IgM的影响[J].石河子大学学报(自然科学版),2017,35(2):152-156. YANG H Y,ZHU X Q,GU X L.Effects of purified Chinese herbal medicinal polysaccharides on Newcastle disease vaccine,T lymphocyte transformation rate and IgG,IgM content in chicken[J].Journal of Shihezi University(Natural Science),2017,35(2):152-156.(in Chinese)
[13] 王德云,胡元亮,孔祥峰,等.中药成分对雏鸡外周血T淋巴细胞转化的影响[J].中国兽医学报,2004,6:578-580. WANG D Y,HU Y L,KONG X F,et al.Effects of Chinese herbal medicinal ingredients on peripheral T lymphocyte transformation in chicken[J].Chinese Journal of Veterinary Science,2004,6:578-580.(in Chinese)
[14] 张海军,徐磊,岳洪源,等.葡多酚对肉鸡生产性能和免疫机能的影响[J].中国畜牧兽医,2012,39(3):99-103. ZHANG H J,XU L,YUE H Y,et al.Effect of grape procyanidins on growth performance and lmmune function in broilers[J].China Animal Husbandry & Veterinary Medicine,2012,39(3):99-103.(in Chinese)
[15] 马忠杰.饲粮葡萄籽比例对多浪羊生长性能、屠宰性能及肉品质的影响[D].阿拉尔:塔里木大学,2022. MA Z J.Effects of dietary grape seed ratio on growth performance,slaughter performance and meat quality of Duolang sheep[D].Alar:Tarim University,2022.(in Chinese)
[16] 刘书东,左文斌,贺艳艳,等.棉酚对多浪羊淋巴细胞凋亡的影响[J].西南大学学报(自然科学版),2011,33(12):7-11. LIU S D,ZUO W B,HE Y Y,et al.Effects of gossypol on apoptosis in Dolang sheep lymphocytes[J].Journal of Southwest University(Natural Science Edition),2011,33(12):7-11.(in Chinese)
[17] 木其尔.沙葱黄酮对肉羊机体抗氧化和免疫功能的影响及其机理研究[D].呼和浩特:内蒙古农业大学,2016. MU Q R.The effects of flavonoids from Allium mongolicun regel on antioxidant capacity,immune function and its mechanism in meat sheep[D].Hohhot:Inner Mongolia Agricultural University,2016.(in Chinese)
[18] SURAI P F.Polyphenol compounds in the chicken/animal diet:From the past to the future[J].Journal of Animal Physiology and Animal Nutrition,2014,98(1):19-31.
[19] 赵昕.葡萄渣替代麦麸对育肥猪免疫性能和肠道健康的影响研究[D].杨凌:西北农林科技大学,2022. ZHAO X.Immune performance and intestinal healthinstead of wheat bran on finishing pigs study on the effect of grape pomace[D].Yangling:Northwest A&F University,2022.(in Chinese)
[20] 杨文军,牟春堂,王鹏举,等.饲粮中添加葡萄籽原花青素对羔羊生长性能、屠宰性能、肉品质及血清抗氧化指标的影响[J].动物营养学报,2020,32(6):2755-2764. YANG W J,MOU C T,WANG P J,et al.Effects of dietary supplementation of grape seed proanthocyanidins on growth performance,slaughter performance,meat quality and serum antioxidant indexes of lambs[J]. Chinese Journal of Animal Nutrition,2020,32(6):2755-2764.(in Chinese)

Effects of Grape Pomace on Lymphocyte Subsets,Lymphocyte Transformation Rate,Apoptosis Rate and Antioxidant Activity in Peripheral Blood of Beef Cows

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.
[2]	WANG Xuefang, GUI Rong, CHEN Gongyi, WANG Wei, ZHANG Hongying, WANG Xuebing, LI Xiao. Clinical Study of Lianhuachaiqin Soluble Powder on Chicken Syndrome of Wind-heat Invading Lung [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3610-3621.
[3]	LI Shinian, LIU Wanning, CHEN Yaping, WANG Jintao. Design of Multi Epitope Vaccine Against Swine Acute Diarrhea Syndrome Coronavirus S, M and E Proteins Based on Immunoinformatics Methods [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1057-1066.
[4]	WANG Xiangyan, XIN Guoqin, XU Haiyan, GU Wei, LAN Jianghua, HAO Muqiang, FENG Gang. Screening of a Strain of Pediococcus pentosus with High Antioxidant Activity [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 179-187.
[5]	XU Baichang, FANG Ziyi, MENG Jinyan, CUI Qinghan, LI Hongman, SI Hongbin. Optimization of Extraction Process and Antioxidant Activity of Polyphenols from Leaves of Broussonetia papyrifera [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1046-1053.
[6]	YAN Yanhua, CHEN Tong, CHEN Zewen, XUE Yang, DU Fangfang, ZHANG Hongying, TONG Chao, WANG Xuebing. Study on the Preventive and Therapeutic Effect of Compound Banqing Soluble Powder on Chicken Infectious Bronchitis [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3379-3388.
[7]	LIU Sheng, ZHANG Jianan, JIA Ning, FANG Mei, DENG Kang, HUANG Weikuan. Analysis of Active Components of Total Flavonoids from the Seeds of Ammopiptanthus mongolicus and Its Effects on Immune Function in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2726-2736.
[8]	XIONG Yunxia, WANG Li, WEN Xiaolu, HU Youjun, YI Hongbo, QIU Yueqin, YANG Xuefen, WU Qiwen, JIANG Zongyong. Effects of adding Ampelopsis grossedentata Extract in Diets on Growth Performance, Blood Biochemical Indexes and Antioxidant Activity in Pig [J]. , 2019, 46(5): 1330-1339.
[9]	CHEN Chuanwei, ZHOU Luli, WANG Dingfa, ZHOU Hanlin. Study on Antioxidant and Anti-inflammatory Effects of Extracts from Piper sarmentosum Roxb. in vitro [J]. , 2019, 46(3): 677-683.
[10]	LIU Meifen, LI Qinnan, MA Zhenyuan, WU Zhiming, BAN Fuguo, YAN Ruoqian, WANG Huajun. Immunopotentiation Effect of Recombinant Chicken Interleukin-6/2 Fusion Protein on Newcastle Disease Virus (LaSota) Live Vaccine [J]. , 2019, 46(2): 590-599.
[11]	ZHANG Yingnan, XU Jing, LUAN Weimin, YANG Shubao. The Localization Changes of CD4⁺ and CD8⁺ T Lymphocytes in Chicken Lung at Different Ages [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3052-3057.
[12]	LI Jianmei, LIU Mei, SHEN Xinyue, DAI Yabin, YU Yan. Analysis of Pathogenicity Difference Between Tibetan Chickens and Recessive White Chickens Following Eimeria tenella Infection [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3058-3064.
[13]	YAO Lili, BAI Lin, TAN Ying, SUN Jiaqi, SHI Dayou, GUO Shining, LIU Cui. Study on Antagonistic Effects of Echinacea purpurea Polysaccharide and Sulfated Echinacea purpurea Polysaccharide on Immunosuppression of Cyclophosphamide in Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(10): 3084-3094.
[14]	CHEN Gang, ZHOU Zhaohai, LIANG Haozhao, YANG Hong, LU Yukui, DENG Hua. Effects of Yupingfeng Polysaccharides on Peyer's Patches Morphology and T Lymphocyte Subsets in Mice [J]. , 2019, 46(1): 313-319.
[15]	MA Zhao, SHANG Yunxia, CHEN Jie, LIU Xiaoting, LIANG Xiaorui, ZHU Xiaoqing, GU Xinli. Effects of Compound Chinese Herbal Medicinal Polysaccharides on Immune Signal Molecules Expression in Different MHC B-LβⅡ Genotypes Chickens Lymphocyte [J]. , 2018, 45(8): 2312-2319.