玉屏风多糖对小鼠派氏结形态结构及其T细胞亚群的影响

doi:10.16431/j.cnki.1671-7236.2019.01.037

Abstract

Abstract:

The effects of Yupingfeng polysaccharides (YPF-P) on the morphology and T cell subsets of Peyer's patches (PPs) were studied to explore its mechanism of immune response to mice intestinal mucosa.Ninety-six SPF mice were randomly divided into 6 groups:Blank control group(0.2 mL saline),YPF-P positive control group (200 mg/kg YPF-P),immunosuppressive group (80 mg/kg cyclophosphamide) and 100 (low),200 (medium) and 400 mg/kg (high) YPF-P groups,feeding for one week.Small intestine PPs were sampled,section and HE stained.The changes of PPs morphological structure were analyzed by image analysis technology.Flow cytometry was used to study the changes in subsets of T lymphocytes isolated from murine PPs.The results showed that YPF-P promoted the growth and development of small intestine PPs in mice.Cyclophosphamide could significantly reduce the area of PPs,the cross-section area of the small intestine and their ratio(P < 0.01).YPF-P could alleviate the damage in the small intestine caused by cyclophosphamide to some extent,increase CD3⁺,CD4⁺ T lymphocytes and CD4⁺/CD8⁺ in PPs lymphocytes (P < 0.05;P < 0.01).The results showed that YPF-P could enhance the intestinal mucosal immune function of cyclophosphamide-induced immunosuppressive mice,and promote the proliferation of related T lymphocytes in PPs,which could enhance the intestinal mucosal function of mice.

Key words: Yupingfeng polysaccharides (YPF-P); mice; Peyer's patches(PPs); intestinal mucosal immune; cyclophosphamide; lymphocyte

CLC Number:

S852.4

CHEN Gang, ZHOU Zhaohai, LIANG Haozhao, YANG Hong, LU Yukui, DENG Hua. Effects of Yupingfeng Polysaccharides on Peyer's Patches Morphology and T Lymphocyte Subsets in Mice[J]. , 2019, 46(1): 313-319.

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Effects of Yupingfeng Polysaccharides on Peyer's Patches Morphology and T Lymphocyte Subsets in Mice

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