梅花鹿早期妊娠不同时期血液转录组分析

doi:10.16431/j.cnki.1671-7236.2024.09.018

Abstract

Abstract: 【Objective】 The objective of this study was to analyze the blood transcriptome data at different stages of early pregnancy in sika deer and identify key genes involved in early pregnancy. 【Method】 Blood samples from female sika deer were collected at 0, 7, 15, and 20 days after artificial insemination.Transcriptome sequencing was performed using the Illumina HiSeq^TM3000C sequencing system to select differentially expressed genes.Principal component analysis and inter-group expression correlation analysis were conducted on blood samples at different time points to identify key stages of early pregnancy in sika deer.DESeq was used to screen differentially expressed genes(DEGs) at each time period, followed by GO, KEGG, and protein-protein interaction network analysis. 【Result】 The transcriptome sequencing results revealed a transcriptional peak in sika deer blood on day 15 of pregnancy.Comparing the groups D7 vs D0, D15 vs D7, and D20 vs D15, 181, 996, and 265 DEGs were identified, respectively.Among them, a total of 7 key genes, including ERAS, MET and KDR, were screened in comparison group D7 vs D0.Comprehensive analysis of GO and KEGG enrichment results indicated that these genes were mainly enriched in biological processes such as cellular response to stimuli and signal transduction, as well as pathways including the PI3K-Akt signaling pathway, and MAPK signaling pathway.A total of 10 key genes, including HRAS, HIF1A, MAPK1, and JAK1, were screened in comparison group D15 vs D7.The genes were mainly enriched in immune system processes, immune response, chemokine signaling pathway, and C-type lectin receptor signaling pathway.A total of 4 key genes, including MAPK1 and MMP2, were identified in comparison group D20 vs D15.The genes were mainly enriched in the estrogen signaling pathway and relaxin signaling pathway. 【Conclusion】 The dynamic expression of blood transcriptome at key time points during early pregnancy in Sika deer was analyzed, and key genes such as KDR, HRAS, MAPK1, and JAK1 were screened, providing reference for the exploration of diagnostic markers for early pregnancy in sika deer.

Key words: sika deer; transcriptome; pregnancy markers; embryonic development; pregnancy recognition

CLC Number:

S825

SUN Huimin, FAN Bingfeng, ZHANG Xulin, SHAO Jing, LIU Lixiang, XU Baozeng. Transcriptomic Analysis of Blood in Early Pregnancy at Different Time Points in Sika Deer[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(9): 3892-3908.

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Transcriptomic Analysis of Blood in Early Pregnancy at Different Time Points in Sika Deer

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