白血病抑制因子对牛卵母细胞体外发育能力的影响

doi:10.16431/j.cnki.1671-7236.2024.02.003

Abstract

Abstract: 【Objective】 This experiment was to explore the effect of leukemia inhibitory factor (LIF) on the development ability of bovine oocytes and early embryo and the expression of multipotent genes.【Method】 The cumulus-oocyte-complexes (COCs) of bovine were divided into 4 groups.In the control group, no LIF solution was added during in vitro maturation (IVM) and embryo in vitro culture (IVC).In treatment group 1 (T1 group), 25 ng/mL LIF was added to the IVM process and no LIF was added to the IVC process.In treatment group 2 (T2 group), the IVM process did not add LIF, and the IVC process added 25 ng/mL LIF.In treatment group 3 (T3 group), 25 ng/mL LIF was added to the whole process of IVM and IVC.The concentration of intracellular free calcium ([Ca²⁺]i) in oocytes was detected by Fluo-3 fluorescence indicator.Oocytes with the first polar body were selected for parthenogenetic activation, and the cleavage rate and blastocyst rate of each group were calculated.Real-time quantitative PCR was used to detect the relative expression of multipotent genes.【Result】 Compared with the control group, intracellular [Ca²⁺]i concentration of bovine oocytes in T1 group was extremely significantly decreased after 8 h of cultivation (P<0.01).After 24 h of cultivation, the intracellular [Ca²⁺]i concentration of bovine oocytes in T1 group was extremely significantly increased (P<0.01).The cleavage rate and blastocyst rate in T1 and T3 groups were significantly higher than those in control group, and the blastocyst rate in T3 group was significantly higher than that in T1 and T2 groups (P<0.05).The results of Real-time quantitative PCR showed that the expression levels of POU5F1 and NANOG genes in T3 group were significantly higher than those in control group, and the expression level of CDX2 gene was significantly lower than that in control group (P<0.05).【Conclusion】 The addition of LIF during IVM could improve the developmental ability of oocytes, and the addition of LIF during the whole process of IVM＋IVC had a positive effect on early embryonic development and the expression of multipotent genes.

Key words: leukemia inhibitory factor; multipotent gene; calcium ions; embryonic development

CLC Number:

S852.2

MO Xianhong, TAO Qinghua, FANG Yi, LIANG Jing, ZHANG Jiayin, LIANG Xiaohang, XU Zhenjun. Effect of Leukemia Inhibitory Factor on Developmental Capacity of Bovine Oocyte[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 462-469.

References

[1] GEARING D P,GOUGH N M,KING J A,et al.Molecular cloning and expression of cDNA encoding a murine myeloid leukaemia inhibitory factor (LIF)[J].EMBO Journal,1987,6(13):3995-4002.
[2] NILSSON E E,KEZELE P,SKINNER M K.Leukemia inhibitory factor (LIF) promotes the primordial to primary follicle transition in rat ovaries[J].Molecular and Cellular Endocrinology,2002,188(1-2):65-73.
[3] FUKUDA M N,SUGIHARA K.Signal transduction in human embryo implantation[J].Cell Cycle,2007,6(10):1153-1156.
[4] DE MATOS D G,MILLER K,SCOTT R,et al.Leukemia inhibitory factor induces cumulus expansion in immature human and mouse oocytes and improves mouse two-cell rate and delivery rates when it is present during mouse in vitro oocyte maturation[J].Fertility and Sterility,2008,90(6):2367-2375.
[5] 陈平.IGF、EGF和LIF调控牦牛IVF和PA胚胎发育及COCs冷冻适应性研究[D].兰州:甘肃农业大学,2017. CHEN P.IGF,EGF and LIF regulate the development of yak embryos produced by IVF and PA and cryo-adaptation of COCs[D].Lanzhou:Gansu Agricultural University,2017.(in Chinese)
[6] MO X H,WU G Q,YUAN D S,et al.Leukemia inhibitory factor enhances bovine oocyte maturation and early embryo development[J].Molecular Reproduction and Development,2014,81(7):608-618.
[7] DANG-NGUYEN T Q,HARAGUCHI S,KIKUCHI K,et al.Leukemia inhibitory factor promotes porcine oocyte maturation and is accompanied by activation of signal transducer and activator of transcription 3[J].Molecular Reproduction and Development,2014,81(3):230-239.
[8] MURATA M,FUKUDA K,ISHIDA H,et al.Leukemia inhibitory factor,a potent cardiac hypertrophic cytokine,enhances L-type Ca²⁺ current and[Ca²⁺]i transient in cardiomyocytes[J].Journal of Molecular and Cellular Cardiology,1999,31(1):237-245.
[9] TAKAHASHI E,FUKUDA K,MIYOSHI S,et al.Leukemia inhibitory factor activates cardiac L-type Ca²⁺ channels via phosphorylation of serine 1829 in the rabbit Cav1.2 subunit[J].Circulation Research,2004,94(9):1242-1248.
[10] 顾全保,徐国江,左嘉客.钙离子在蟾蜍卵母细胞成熟分裂过程中的作用[J].实验生物学报,1988,2:51-60. GU Q B,XU G J,ZUO J K.The effect of calcium on toad oocyte maturation induced by progesterone[J].Journal of Molecular Cell Biology,1988,2:51-60.(in Chinese)
[11] 侯云鹏,李雪.钙离子在哺乳动物卵母细胞发育关键时期的作用[J].中国农业大学学报,2019,24(1):46-51. HOU Y P,LI X.Role of calcium ion in the critical development periods of mammalian oocytes[J].Journal of China Agricultural University,2019,24(1):46-51.(in Chinese)
[12] TAKAHASHI K,YAMANAKA S.A decade of transcription factor-mediated reprogramming to pluripotency[J].Nature Reviews Molecular Cell Biology,2016,17(3):183-193.
[13] ZHU Y,LOHNES D.Regulation of axial elongation by Cdx[J].Developmental Biology,2022,483:118-127.
[14] SAMADIAN A,HESARAKI M,MOLLAMLHAMMADI S,et al.Temporal gene expression and DNA methylation during embryonic stem cell derivation[J].Cell Journal,2018,20(3):361-368.
[15] MENCHERO S,SAINZ D A J,MANZANARES M.Our first choice:Cellular and genetic underpinnings of trophectoderm identity and differentiation in the mammalian embryo[J].Current Topics in Developmental Biology,2018,128:59-80.
[16] ORTEGA M S,KELLEHER A M,O'NEIL E,et al.NANOG is required to form the epiblast and maintain pluripotency in the bovine embryo[J].Molecular Reproduction and Development,2020,87(1):152-160.
[17] MEI X L,ZHAO H H,AI H H,et al.Modaline sulfate promotes Oct4 expression and maintains self-renewal and pluripotency of stem cells through JAK/STAT3 and Wnt signaling pathways[J].Cell and Bioscience,2021,11(1):156-173.
[18] AGHAJANOVA L.Leukemia inhibitory factor and human embryo implantation[J].Annals of the News York Academy of Sciences,2004,1034:176-183.
[19] CADORET V,JARRIER-GAILLARD P,PAPILLIER P,et al.Leukaemia inhibitory factor modulates the differentiation of granulosa cells during sheep in vitro preantral to antral follicle development and improves oocyte meiotic competence[J].Molecular Human Reproduction,2021,27(9):gaab051.
[20] TIAN H,QI Q,YAN F F,et al.Enhancing the developmental competence of prepubertal lamb oocytes by supplementing the in vitro maturation medium with sericin and the fibroblast growth factor 2-leukemia inhibitory factor-insulin-like growth factor 1 combination[J].Theriogenology,2021,159:13-19.
[21] ECKERT J,NIECKERT J,NIEMANN H.mRNA expression of leukaemia inhibitory factor (LIF) and its receptor subunits glycoprotein 130 and LIF-receptor-beta in bovine embryos derived in vitro or in vivo[J].Molecular Human Reproduction,1998,4(10):957-965.
[22] LIANG S L,ZHAO Q J,LI X C,et al.Dynamic analysis of Ca²⁺ level during bovine oocytes maturation and early embryonic development[J].Journal of Veterinary Science,2011,12(2):133-142.
[23] RAMOS LEAL G,SANTOS MONTEIRO C A,SOUZA-FABJAN J M G,et al.Role of cAMP modulator supplementations during oocyte in vitro maturation in domestic animals[J].Animal Reproduction Science,2018,199:1-14.
[24] 石培欣.生长因子和C型钠肽对山羊卵母细胞成熟及早期胚胎发育的影响[D].呼和浩特:内蒙古大学,2021. SHI P X.Effects of growth factors and C-type natriuretic peptide on goat oocyte maturation and early embryo development[D].Hohhot:Inner Mongolia University,2021.(in Chinese)
[25] 于婷婷.卵母细胞成熟条件及供体细胞传代次数对猪体细胞核移植影响的研究[D].长春:吉林大学,2018. YU T T.The comparative study of different factors on oocyte maturation and the effect of donor cell passages on porcine SCNT[D].Changchun:Jilin University,2018.(in Chinese)
[26] 牛至寒.猪卵母细胞体外成熟及胚胎培养液的优化[D].杨凌:西北农林科技大学,2022. NIU Z H.In vitro maturation of porcine oocytes and optimization of embryo culture medium[D]. Yangling:Northwest A&F University,2022.(in Chinese)
[27] ESWARI S,SAI KUMAR G,SHARMA G T.Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRbeta) in buffalo preimplantation embryos produced in vitro:Markers of successful embryo implantation[J].Zygote,2013,21(2):203-213.
[28] VERNERI P,VAZQUEZ ECHEGARAY C,OSES C,et al.Dynamical reorganization of the pluripotency transcription factors Oct4 and Sox2 during early differentiation of embryonic stem cells[J].Scientific Reports,2020,10(1):5195-5207.
[29] SIMMET K,ZAKHARTCHENKO V,PHILIPPOU-MASSIER J,et al.OCT4/POU5F1 is required for NANOG expression in bovine blastocysts[J].Proceedings of the National Academy of Sciences of the United States of America,2018,115(11):2770-2775.
[30] SCHIFFMACHER A T,KEEFER C L.CDX2 regulates multiple trophoblast genes in bovine trophectoderm CT-1 cells[J].Molecular Reproduction and Development,2013,80(10):826-839.
[31] CERVERA R P,MARTI-GUTIERREZ N,ESCORIHUELA E,et al.Trichostatin A affects histone acetylation and gene expression in porcine somatic cell nucleus transfer embryos[J].Theriogenology,2009,72(8):1097-1110.
[32] IKEDA S,TATEMIZO A,IWAMOTO D,et al.Enhancement of histone acetylation by trichostatin A during in vitro fertilization of bovine oocytes affects cell number of the inner cell mass of the resulting blastocysts[J].Zygote,2009,17(3):209-215.
[33] WASIELAK M,WI[XCE弯.eps;% 125% 125;S-+0.3mm;X-+2mm;Z-0.5mm;Y-0.8mm,SQ;P]SAK T,BOGACKA I,et al.Maternal effect gene expression in porcine metaphase Ⅱ oocytes and embryos in vitro:Effect of epidermal growth factor,interleukin-1β and leukemia inhibitory factor[J].Zygote,2017,25(2):120-130.
[34] 方园.猪早期胚胎体外培养体系优化及对干细胞建系影响的研究[D].哈尔滨:东北农业大学,2022. FANG Y.Optimization of in vitro culture system of porcine early embryo and its influence on the establishment of embryonic stem cell lines[D].Harbin:Northeast Agricultural University,2022.(in Chinese)
[35] ALBERIO R.Regulation of cell fate decisions in early mammalian embryos[J].Annual Review of Animal Biosciences,2020,8:377-393.

Effect of Leukemia Inhibitory Factor on Developmental Capacity of Bovine Oocyte

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